Heterologous Production of Desferrioxamines with a Fusion Biosynthetic Gene Cluster

2013 ◽  
Vol 77 (12) ◽  
pp. 2467-2472 ◽  
Author(s):  
Masaki J. FUJITA ◽  
Ryuichi SAKAI
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

scholarly journals Heterologous Biosynthesis and Genomics-Driven Derivatization of Fungal Bioactive Sesterterpenoid Variecolin

2021 ◽  
Author(s):  
Dexiu Yan ◽  
Jemma Arakelyan ◽  
Teng Wan ◽  
Tsz Ki Chan ◽  
Dohyun Ahn ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Anticancer Activity ◽  
Gene Cluster ◽  
Aspergillus Oryzae ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Aspergillus Aculeatus ◽  
Heterologous Biosynthesis

The biosynthetic gene cluster of fungal bioactive sesterterpenoids, variecolin (1) and variecolactone (2), was identified in Aspergillus aculeatus ATCC 16872. Heterologous production of 1 and 2 was achieved in Aspergillus oryzae by expressing the sesterterpene synthase VrcA and the cytochrome P450 VrcB. Intriguingly, the replacement of VrcB with homologous P450s from other fungal terpenoid pathways yielded three new variecolin analogues, one of which exhibited potent anticancer activity comparable to that of 1.

Heterologous production of bisucaberin using a biosynthetic gene cluster cloned from a deep sea metagenome

2012 ◽  
Vol 8 (2) ◽  
pp. 482-485 ◽  
Author(s):  
Masaki J. Fujita ◽  
Nobutada Kimura ◽  
Hisayoshi Yokose ◽  
Masami Otsuka
Keyword(s):  
Gene Cluster ◽  
Deep Sea ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

scholarly journals Heterologous Production of Fosfomycin and Identification of the Minimal Biosynthetic Gene Cluster

2006 ◽  
Vol 13 (11) ◽  
pp. 1171-1182 ◽  
Author(s):  
Ryan D. Woodyer ◽  
Zengyi Shao ◽  
Paul M. Thomas ◽  
Neil L. Kelleher ◽  
Joshua A.V. Blodgett ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

Heterologous Production of Glidobactins/Luminmycins inEscherichia coliNissle Containing the Glidobactin Biosynthetic Gene Cluster fromBurkholderiaDSM7029

ChemBioChem ◽  
2014 ◽  
Vol 15 (15) ◽  
pp. 2221-2224 ◽  
Author(s):  
Xiaoying Bian ◽  
Fan Huang ◽  
Hailong Wang ◽  
Thorsten Klefisch ◽  
Rolf Müller ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

Discovery and Heterologous Production of Sarubicins and Quinazolinone C-Glycosides with Protecting Activity for Cardiomyocytes

2021 ◽  
Author(s):  
Mingming Yu ◽  
Jianying Luo ◽  
Dan Luo ◽  
Qiang He ◽  
Yijun Yan ◽  
...  
Keyword(s):  
Natural Products ◽  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Pharmaceutical Agents

Glycosylated natural products and their derivatives are important pharmaceutical agents. Genome mining of C-glycosylated metabolites biosynthetic gene cluster (BGC) from a soil-derived Streptomyces led to the isolation of sarubicins A...

Heterologous production of new protease inhibitory peptide marinostatin E

2021 ◽  
Vol 85 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Kohta Unno ◽  
Hiroyuki Nakagawa ◽  
Shinya Kodani
Keyword(s):  
Gene Cluster ◽  
Marine Bacterium ◽  
Expression Vector ◽  
Biosynthetic Gene Cluster ◽  
Tandem Mass ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Inhibitory Peptide ◽  
Chemical Treatments ◽  
Inhibitory Activities

Abstract Bicyclic peptides, marinostatins, are protease inhibitors derived from the marine bacterium Algicola sagamiensis. The biosynthetic gene cluster of marinostatin was previously identified, although no heterologous production was reported. In this report, the biosynthetic gene cluster of marinostatin (mstA and mstB) was cloned into the expression vector pET-41a(+). As a result of the coexpression experiment, a new analogous peptide named marinostatin E was successfully produced using Escherichia coli BL21(DE3). The structure of marinostatin E was determined by a combination of chemical treatments and tandem mass spectrometry experiments. Marinostatin E exhibited inhibitory activities against chymotrypsin and subtilisin with an IC50 of 4.0 and 39.6 μm, respectively.

scholarly journals Biosynthesis and Heterologous Expression of Cacaoidin, the First Member of the Lanthidin Family of RiPPs

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 403
Author(s):  
Fernando Román-Hurtado ◽  
Marina Sánchez-Hidalgo ◽  
Jesús Martín ◽  
Francisco Javier Ortiz-López ◽  
Olga Genilloud
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Class V ◽  
First Report ◽  
Genome Databases ◽  
Complete Identification ◽  
Bioinformatic Tool

Cacaoidin is produced by the strain Streptomyces cacaoi CA-170360 and represents the first member of the new lanthidin (class V lanthipeptides) RiPP family. In this work, we describe the complete identification, cloning and heterologous expression of the cacaoidin biosynthetic gene cluster, which shows unique RiPP genes whose functions were not predicted by any bioinformatic tool. We also describe that the cacaoidin pathway is restricted to strains of the subspecies Streptomyces cacaoi subsp. cacaoi found in public genome databases, where we have also identified the presence of other putative class V lanthipeptide pathways. This is the first report on the heterologous production of a class V lanthipeptide.

Anacyclamide D8P, a prenylated cyanobactin from a Sphaerospermopsis sp. cyanobacterium

2017 ◽  
Author(s):  
Joana Martins ◽  
Niina Leikoski ◽  
Matti Wahlsten ◽  
Joana Azevedo ◽  
Jorge Antunes ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Cyclic Peptides ◽  
Biosynthetic Gene Cluster ◽  
The Novel ◽  
Tyrosine Residue ◽  
Biosynthetic Gene ◽  
Post Translational Modification ◽  
Biological Assays ◽  
Mass Spectrometric Data ◽  
Spectrometric Data

Cyanobactins are a family of linear and cyclic peptides produced through the post-translational modification of short precursor peptides. Anacyclamides are macrocyclic cyanobactins with a highly diverse sequence that are common in the genus <i>Anabaena</i>. A mass spectrometry-based screening of potential cyanobactin producers led to the discovery of a new prenylated member of this family of compounds, anacyclamide D8P (<b>1</b>), from <i>Sphaerospermopsis</i> sp. LEGE 00249. The anacyclamide biosynthetic gene cluster (<i>acy</i>) encoding the novel macrocyclic prenylated cyanobactin, was sequenced. Heterologous expression of the acy gene cluster in <i>Escherichia</i> <i>coli</i> established the connection between genomic and mass spectrometric data. Unambiguous establishment of the type and site of prenylation required the full structural elucidation of <b>1</b> using Nuclear Magnetic Resonance (NMR), which demonstrated that a forward prenylation occurred on the tyrosine residue. Compound <b>1</b> was tested in pharmacologically or ecologically relevant biological assays and revealed moderate antimicrobial activity towards the fouling bacterium <i>Halomonas aquamarina</i> CECT 5000.<br>

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