Investigating plant cell wall components that affect biomass recalcitrance in poplar and switchgrass

2013 ◽  
Vol 6 (3) ◽  
pp. 898 ◽  
Author(s):  
Jaclyn D. DeMartini ◽  
Sivakumar Pattathil ◽  
Jeffrey S. Miller ◽  
Hongjia Li ◽  
Michael G. Hahn ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Biomass Recalcitrance ◽  
Cell Wall Components ◽  
Wall Components

scholarly journals Regulation of acetylation of plant cell wall components is complex and responds to external stimuli

2019 ◽  
Vol 15 (1) ◽  
pp. 1687185 ◽  
Author(s):  
S. A. Sinclair ◽  
S. Gille ◽  
M. Pauly ◽  
U. Krämer
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Components ◽  
External Stimuli ◽  
Wall Components

Binding selectivity of dietary polyphenols to different plant cell wall components: Quantification and mechanism

2017 ◽  
Vol 233 ◽  
pp. 216-227 ◽  
Author(s):  
Anh Dao T. Phan ◽  
Bernadine M. Flanagan ◽  
Bruce R. D'Arcy ◽  
Michael J. Gidley
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Binding Selectivity ◽  
Cell Wall Components ◽  
Dietary Polyphenols ◽  
Wall Components

Acquired Resistance in Hypersensitive Tobacco against Tobacco Mosaic Virus, Induced by Plant Cell Wall Components

1985 ◽  
Vol 113 (2) ◽  
pp. 165-170 ◽  
Author(s):  
P. W. Modderman ◽  
C. P. Schot ◽  
F. M. Klis ◽  
D. H. Wieringa-Brants
Keyword(s):  
Cell Wall ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Acquired Resistance ◽  
Cell Wall Components ◽  
Wall Components

Self-assembly Mechanisms in Plant Cell Wall Components

2015 ◽  
Vol 3 (1) ◽  
pp. 56-72 ◽  
Author(s):  
Yogesh K. Murugesan ◽  
Damiano Pasini ◽  
Alejandro D. Rey
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Cell Wall Components ◽  
Wall Components

scholarly journals Activating Intrinsic Carbohydrate-Active Enzymes of the Smut Fungus Ustilago maydis for the Degradation of Plant Cell Wall Components

2016 ◽  
Vol 82 (17) ◽  
pp. 5174-5185 ◽  
Author(s):  
Elena Geiser ◽  
Michèle Reindl ◽  
Lars M. Blank ◽  
Michael Feldbrügge ◽  
Nick Wierckx ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Hydrolytic Enzymes ◽  
Value Added ◽  
Ustilago Maydis ◽  
Smut Fungus ◽  
Content Type ◽  
Cell Wall Components ◽  
Wall Components

ABSTRACTThe microbial conversion of plant biomass to valuable products in a consolidated bioprocess could greatly increase the ecologic and economic impact of a biorefinery. Current strategies for hydrolyzing plant material mostly rely on the external application of carbohydrate-active enzymes (CAZymes). Alternatively, production organisms can be engineered to secrete CAZymes to reduce the reliance on externally added enzymes. Plant-pathogenic fungi have a vast repertoire of hydrolytic enzymes to sustain their lifestyle, but expression of the corresponding genes is usually highly regulated and restricted to the pathogenic phase. Here, we present a new strategy in using the biotrophic smut fungusUstilago maydisfor the degradation of plant cell wall components by activating its intrinsic enzyme potential during axenic growth. This fungal model organism is fully equipped with hydrolytic enzymes, and moreover, it naturally produces value-added substances, such as organic acids and biosurfactants. To achieve the deregulated expression of hydrolytic enzymes during the industrially relevant yeast-like growth in axenic culture, the native promoters of the respective genes were replaced by constitutively active synthetic promoters. This led to an enhanced conversion of xylan, cellobiose, and carboxymethyl cellulose to fermentable sugars. Moreover, a combination of strains with activated endoglucanase and β-glucanase increased the release of glucose from carboxymethyl cellulose and regenerated amorphous cellulose, suggesting that mixed cultivations could be a means for degrading more complex substrates in the future. In summary, this proof of principle demonstrates the potential applicability of activating the expression of native CAZymes from phytopathogens in a biocatalytic process.IMPORTANCEThis study describes basic experiments that aim at the degradation of plant cell wall components by the smut fungusUstilago maydis. As a plant pathogen, this fungus contains a set of lignocellulose-degrading enzymes that may be suited for biomass degradation. However, its hydrolytic enzymes are specifically expressed only during plant infection. Here, we provide the proof of principle that these intrinsic enzymes can be synthetically activated during the industrially relevant yeast-like growth. The fungus is known to naturally synthesize valuable compounds, such as itaconate or glycolipids. Therefore, it could be suited for use in a consolidated bioprocess in which more complex and natural substrates are simultaneously converted to fermentable sugars and to value-added compounds in the future.

scholarly journals Effect of lignin linkages with other plant cell wall components on in vitro and in vivo neutral detergent fiber digestibility and rate of digestion of grass forages

2017 ◽  
Vol 100 (10) ◽  
pp. 8119-8131 ◽  
Author(s):  
E. Raffrenato ◽  
R. Fievisohn ◽  
K.W. Cotanch ◽  
R.J. Grant ◽  
L.E. Chase ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Neutral Detergent Fiber ◽  
Cell Wall Components ◽  
Fiber Digestibility ◽  
Wall Components

Metabolic profile of caecal micro-organisms from rats fed indigestible plant cell-wall components

1983 ◽  
Vol 21 (1) ◽  
pp. 25-29 ◽  
Author(s):  
I.R. Rowland ◽  
A. Wise ◽  
A.K. Mallett
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Metabolic Profile ◽  
Plant Cell Wall ◽  
Cell Wall Components ◽  
Micro Organisms ◽  
Wall Components

scholarly journals Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

2013 ◽  
Vol 80 (2) ◽  
pp. 574-585 ◽  
Author(s):  
Cherie J. Ziemer
Keyword(s):  
Cell Wall ◽  
Continuous Culture ◽  
Plant Cell ◽  
Intestinal Microbiota ◽  
Plant Cell Wall ◽  
Bacterial Isolates ◽  
Content Type ◽  
Cell Wall Components ◽  
Cultured Bacteria ◽  
Wall Components

ABSTRACTOne of the functions of the mammalian large intestinal microbiota is the fermentation of plant cell wall components. In ruminant animals, the majority of their nutrients are obtained via pregastric fermentation; however, up to 20% can be recovered from microbial fermentation in the large intestine. Eight-week continuous culture enrichments of cattle feces with cellulose and xylan-pectin were used to isolate bacteria from this community. A total of 459 bacterial isolates were classified phylogenetically using 16S rRNA gene sequencing. Six phyla were represented:Firmicutes(51.9%),Bacteroidetes(30.9%),Proteobacteria(11.1%),Actinobacteria(3.5%),Synergistetes(1.5%), andFusobacteria(1.1%). The majority of bacterial isolates had <98.5% identity to cultured bacteria with sequences in the Ribosomal Database Project and thus represent new species and/or genera. Within theFirmicutesisolates, most were classified in the familiesLachnospiraceae,Ruminococcaceae,Erysipelotrichaceae, andClostridiaceaeI. The majority of theBacteroideteswere most closely related toBacteroides thetaiotaomicron,B. ovatus, andB. xylanisolvensand members of thePorphyromonadaceaefamily. Many of theFirmicutesandBacteroidetesisolates were related to species demonstrated to possess enzymes which ferment plant cell wall components; the others were hypothesized to cross-feed these bacteria. The microbial communities that arose in these enrichment cultures had broad bacterial diversity. With over 98% of the isolates not represented as previously cultured, there are new opportunities to study the genomic and metabolic capacities of these members of the complex intestinal microbiota.

scholarly journals Uncovering the Cultivable Microbial Diversity of Costa Rican Beetles and Its Ability to Break Down Plant Cell Wall Components

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113303 ◽  
Author(s):  
Gabriel Vargas-Asensio ◽  
Adrian Pinto-Tomas ◽  
Beatriz Rivera ◽  
Myriam Hernandez ◽  
Carlos Hernandez ◽  
...  
Keyword(s):  
Cell Wall ◽  
Microbial Diversity ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Costa Rican ◽  
Cell Wall Components ◽  
Wall Components
Sign in / Sign up

Export Citation Format

Share Document