Kinetic characterization of the retinoic X receptor binding to specific and unspecific DNA oligoduplexes with a quartz crystal microbalance

The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3434-3440
Author(s):  
Rogério M. M. Rodrigues ◽  
Jorge de-Carvalho ◽  
Guilherme N. M. Ferreira
Keyword(s):  
Quartz Crystal Microbalance ◽  
Receptor Binding ◽  
Quartz Crystal ◽  
Kinetic Characterization ◽  
Kinetics Of

A frequency at the susceptance minimum (fBmin) is applied to assess the kinetics of RXRα to specific and non-specific oligoduplexes.

Kinetic characterization of small DNA-binding molecules interacting with a DNA strand on a quartz crystal microbalance

2016 ◽  
Vol 492 ◽  
pp. 34-42 ◽  
Author(s):  
Hiroyuki Furusawa ◽  
Hajime Nakayama ◽  
Mariko Funasaki ◽  
Yoshio Okahata
Keyword(s):  
Dna Binding ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Kinetic Characterization ◽  
Dna Strand

Kinetics of Water Sorption in NafionThin Films − Quartz Crystal Microbalance Study

2001 ◽  
Vol 105 (33) ◽  
pp. 7979-7983 ◽  
Author(s):  
Petr Krtil ◽  
Antonín Trojánek ◽  
Zdeněk Samec
Keyword(s):  
Quartz Crystal Microbalance ◽  
Water Sorption ◽  
Quartz Crystal ◽  
Kinetics Of

Quartz crystal microbalance characterization of electrochemical doping of polyaniline films

1993 ◽  
Vol 61 (3) ◽  
pp. 291-296 ◽  
Author(s):  
R.M. Torresi ◽  
S.I.Cordoba de Torresi ◽  
C. Gabrielli ◽  
M. Keddam ◽  
H. Takenouti
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Electrochemical Doping ◽  
Polyaniline Films

Characterization of Jumping-Droplet Condensation on Nanostructured Surfaces With Quartz Crystal Microbalance

2017 ◽  
Author(s):  
Junwei Su ◽  
Hamed Esmaeilzadeh ◽  
Chefu Su ◽  
Majid Charmchi ◽  
Marina Ruths ◽  
...  
Keyword(s):  
Frequency Shift ◽  
Quartz Crystal Microbalance ◽  
Microscopic Observation ◽  
Quartz Crystal ◽  
Structured Surfaces ◽  
Nanostructured Surfaces ◽  
Acoustic Technique ◽  
Flow Condensation ◽  
Droplet Condensation

The spontaneously jumping motion of condensed droplets by coalescence on superhydrophobic surfaces has been an active area of research due to its great potential for enhancing the condensation efficiency. Despite a considerable amount of microscopic observations, the interfacial wetting characterization during jumping-droplet condensation is still notably lacking. This work focuses on applying a novel acoustic sensor - quartz crystal microbalance (QCM), to characterize the interfacial wetting on nanostructured surfaces during jumping-droplet condensation. Copper oxide nanostructures were generated on the surface of QCM with a chemical etching method. Based on the geometry of the nanostructures, we modified a theoretical model to reveal the relationship between the frequency shift of the QCM and the wetting states of the surfaces. It was found that the QCM is extremely sensitive to the penetrated liquid in the structured surfaces. Then, the QCM with nanostructured surface was tested on a customed flow condensation setup. The dynamic interfacial wetting characteristics were quantified by the normalized frequency shift of the QCM. Combined with microscopic observation of the corresponding drop motion, we demonstrated that partial wetting (PW) droplets with an about 25% penetrated area underwent spontaneously jumping by coalescence. However, the PW droplets no longer jumped when the penetrated area exceeds 50% due to the stronger adhesion between liquid and the surface. It shows that the characterization of the penetrated liquid in micro/nanostructures, which is very challenging for microscopic observation, can be easily carried out by this acoustic technique.

A Quartz Crystal Microbalance Characterization of Metal‐Oil Interfaces and Interactions with Wax Molecules

2008 ◽  
Vol 29 (5) ◽  
pp. 775-782 ◽  
Author(s):  
Kristofer Paso ◽  
Thomas Kompalla ◽  
Narve Aske ◽  
Johan Sjöblom
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal

SURFACE PLASMON RESONANCE SPECTROSCOPY AND QUARTZ CRYSTAL MICROBALANCE STUDY OF PROTEIN-DNA INTERACTIONS IN HORMONE RECEPTOR BIOLOGY

COSMOS ◽  
2009 ◽  
Vol 05 (01) ◽  
pp. 79-95
Author(s):  
XIAODI SU
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Quartz Crystal Microbalance ◽  
Conformational Changes ◽  
Plasmon Resonance ◽  
Quartz Crystal ◽  
Resonance Spectroscopy ◽  
Dna Interactions ◽  
Protein Dna Interactions

Surface plasmon resonance (SPR) spectroscopy and quartz crystal microbalance (QCM) are surface sensitive analytical techniques capable of real-time monitoring of biomolecular interactions. In this article we review our past work on the use of these two techniques for studying protein–DNA interactions, exemplified with estrogen receptors (ER) and their response elements (ERE). Various assay schemes have been developed for a comprehensive characterization of ER–ERE interactions in terms of sequence specificity, binding affinity, stoichiometry, ligand effects on binding dynamics and conformational changes in the proteins and DNA. These are all important characteristics underlining the mechanism of ER-mediated gene transcription. With these studies we have made the following demonstrations to describe the advantages of these two techniques, namely (i) SPR technique is superior and more versatile than conventional (electrophoretic mobility shift assay) EMSA for studying protein-DNA interactions; (ii) QCM is an alternative tool for studying conformational changes in protein–DNA complexes and (iii) combinational SPR and QCM analysis provides additional characterization of biomolecular films, e.g. film thickness, water content, and conformation rigidity etc.

scholarly journals Frequency-shift vs phase-shift characterization of in-liquid quartz crystal microbalance applications

2011 ◽  
Vol 82 (6) ◽  
pp. 064702 ◽  
Author(s):  
Y. J. Montagut ◽  
J. V. García ◽  
Y. Jiménez ◽  
C. March ◽  
A. Montoya ◽  
...  
Keyword(s):  
Phase Shift ◽  
Frequency Shift ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal
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