A systematic quality control method of Huangqi decoction: simultaneous determination of eleven flavonoids and seven triterpenoid saponins by UHPLC-MS

2014 ◽  
Vol 6 (13) ◽  
pp. 4593-4601 ◽  
Author(s):  
Hui-Long Luo ◽  
Jie Zhong ◽  
Fu-Yuan Ye ◽  
Qian Wang ◽  
Yue-Ming Ma ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Control Method ◽  
Bioactive Components ◽  
Triterpenoid Saponins ◽  
Quality Control Method

A novel UHPLC-MS method was developed for the quantitative analysis of 18 major bioactive components from Huangqi decoction (HQD).

New Determination Method of Two Bioactive Saponins in Lysimachia capillipes Hemsl. by Quantitative Analysis of Multi-Components by Single-Marker

2021 ◽  
Author(s):  
Xiaoyong Zhang ◽  
Xuezhao Chen ◽  
Juan Jin ◽  
Minghua Gong ◽  
Qiang He ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
High Performance ◽  
Reference Standards ◽  
Bioactive Components ◽  
Evaporative Light Scattering Detector ◽  
Evaporative Light Scattering ◽  
External Standard ◽  
Single Marker

Abstract Capilliposide B (CPS-B) and Capilliposide C (CPS-C), as the key components in Lysimachia capillipes Hemsl., increasingly aroused the interest and research concern of many researchers due to the good bioactivities. Nowadays, the reference standards of CPS-B and CPS-C yield were very limited. Due to the deficit of reference standards, the determination could be difficult to carry out, and the quality control and evaluation would be restrained afterwards. To solve this urgent problem, a quantitative analysis of multi-components by single-marker (QAMS) method was proposed and established based on high-performance liquid-chromatography tandem evaporative light-scattering detector. In this QAMS method, the content of the two bioactive components could be calculated by buddlejasaponin IV, which is applied as an external standard and readily obtained. And the methodological experiments were evaluated and indicated accuracy, stability and feasibility of this QAMS method. Therefore, in this study, this built method would properly meet the requirement of determination of CPS-B, CPS-C and quality control of the L. capillipes Hemsl. plant.

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

scholarly journals Quality-control method for the determination of biological activity of engineered calcineurin subunit B

2016 ◽  
Vol 59 (6) ◽  
pp. 584-588
Author(s):  
Xinchang Shi ◽  
Huan Yang ◽  
Li Xu ◽  
Xiang Li ◽  
Zongwen Huang ◽  
...  
Keyword(s):  
Quality Control ◽  
Biological Activity ◽  
Control Method ◽  
Quality Control Method ◽  
Subunit B

Simultaneous Determination of Five Irdoid Glycosides and Three Flavonoid Glycosides in Hedyotis Diffusa Wild by UPLC-UV with Ultrasound-Assisted Extraction

2019 ◽  
Vol 15 (7) ◽  
pp. 808-818
Author(s):  
Xuliang Huang ◽  
Youjia Wu ◽  
Xiaoying Zhang ◽  
Bing Chen ◽  
Hongbin Luo ◽  
...  
Keyword(s):  
Quality Control ◽  
Herbal Medicine ◽  
Simultaneous Determination ◽  
Quantitative Methods ◽  
Control Method ◽  
Chinese Herbs ◽  
Quality Analysis ◽  
Flavonoid Glycosides ◽  
Hedyotis Diffusa

Background: Hedyotis diffusa is one of the most extensively used Chinese herbs for the treatment of varieties of cancers in the folk of China. The ingredients of the preparation are still not very clear and the quality control method of the herb medicine requires further improvement. Introduction: In this paper, the quantitative methods for the quality control of H. diffusa were originally developed by using UPLC-UV. Methods: Using the five irdoid glycosides and three flavonoid glycosides previously obtained from H. diffusa as index components, referring to 10-Acetyl Scandoside Methyl Ester (ASME), Scandoside (SD), Deacetyl Aspemloside (DA), Asperulosidic Acid (AA), Asperuloside (AD), Qurecetin-3-OSophoroside (QS), Quercetin-3-O-[2-(6-O-E-Sinapoyl-β-D-Glucopyranosyl]-β-D-Glucopyranosyl (QESGG), and Quercetin-3-O-[2-O-(6-O-E-Feruloyl)-β-D-Glucopyranosyl]-β-Glucopyanoside (QEFGG), the extraction conditions, including solvents, durations, and cycles were optimized firstly. Subsequently, a new UPLC-UV method was originally established to simultaneously determine the eight ingredients in the Chinese herbal medicine. Results: The validated method exhibited good linearity (R2 > 0.999), repeatability (RSD < 3.01%), intra- and inter-day precision (RSD <6.19%) and recoveries (96.31% to 104.22%) for the simultaneous determination of the eight ingredients studied and had been used for the quality analysis of 6 batches of H. diffusa sameples. Conclusion: The established UPLC-UV method could serve as a rapid and effective tool for quality evaluation of the herbal medicine, H. diffusa.

scholarly journals An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Jin Li ◽  
Qiu-Hong Zhang ◽  
Jun He ◽  
Er-wei Liu ◽  
Xiu-mei Gao ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Control Method ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Bioactive Constituents ◽  
Linear Gradient ◽  
Radix Angelicae Pubescentis ◽  
Aqueous Ammonium Acetate

An improved LC-MS/MS method was developed for simultaneous determination of eleven bioactive constituents of Radix Angelicae Pubescentis and its related preparations. It was the first report on the quantification of bioactive constituents in different preparations of Radix Angelicae Pubescentis by LC-MS/MS analytical method. These samples were separated with an Agilent Zorbax Extend reversed-phase C18 column (1.8 μm, 4.6 × 100 mm) by linear gradient elution using aqueous ammonium acetate and acetonitrile as mobile phase. The flow rate was 0.3 mL min−1. The eleven bioactive constituents showed good regression(R>0.990)within test ranges and the recoveries were in the range of 87.1–110%. The limit of detections and quantifications for most of the major constituents were less than 0.5 and 1.0 ng mL−1, respectively. All results indicated that the developed method could be readily utilized as a suitable quality control method for Radix Angelicae Pubescentis and related preparations.

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