Precise micromolar-level glucose determination using a glucose test strip for quick and approximate millimolar-level estimation

2014 ◽  
Vol 6 (23) ◽  
pp. 9509-9513 ◽  
Author(s):  
Ryoichi DOI
Keyword(s):  
Reaction Time ◽  
Test Strip ◽  
Test Strips ◽  
Glucose Determination ◽  
Glucose Test

Various test strips for the quick and rough estimation of analytes are commonly and reasonably available. When the reaction time of 30 seconds indicated in the manufacturer’s instructions was extended to three hours, the sensitivity increased 56-fold.

scholarly journals Analytical Characterization of Electrochemical Biosensor Test Strips for Measurement of Glucose in Low-Volume Interstitial Fluid Samples

1999 ◽  
Vol 45 (9) ◽  
pp. 1665-1673 ◽  
Author(s):  
Michael E Collison ◽  
Philip J Stout ◽  
Tatyana S Glushko ◽  
Kristen N Pokela ◽  
Debra J Mullins-Hirte ◽  
...  
Keyword(s):  
Interstitial Fluid ◽  
Glucose Monitoring ◽  
Test Strip ◽  
Sample Volume ◽  
Diabetic Subject ◽  
Glucose Measurement ◽  
Γ Irradiation ◽  
Glucose Response ◽  
Test Strips ◽  
Glucose Test

Abstract Background: Minimally invasive interstitial fluid (ISF) sampling and glucose measurement technologies were integrated into a hand-held device for diabetic glucose monitoring investigations. Methods: Conventional electrochemical test strip technology (Bayer Glucometer Elite®) was adapted to measure glucose in small (0.5–2.0 μL) samples of ISF. Test strip glucose measurements were performed on a commercial potentiostat and were compared to various reference glucose methodologies (YSI 2300 analyzer, microhexokinase procedure, Bayer Glucometer Elite). Characterizations of the integrated ISF sampling-glucose test strip design included accuracy and precision in various sample media (saline, ISF surrogates, diabetic ISF samples), sample volume dependence, test strip sterilization studies (electron beam, γ irradiation), and diabetic ISF sampling and glucose measurements. Results: Glucose measurements were free from significant media effects. Sample volume variations (0.6–3.2 μL) revealed only modest dependence of glucose measurement bias on sample volume (−1.5% per microliter). Sterilization treatments had only a minor impact on glucose response and test strip aging and no significant impact on interferent responses of the glucose test strips. Diabetic subject testing under minimum fasting conditions of at least 2 h with integrated ISF sampling and glucose measurement gave low ISF glucose measurement imprecision (CV, 4%) and mean glucose results that were indistinguishable from reference (microhexokinase) ISF glucose measurements and from capillary blood glucose measurements (Glucometer Elite). Conclusions: Conventional single-use, electrochemical glucose test strip and ISF collection technologies can be readily integrated to provide real-time ISF sampling and glucose measurements for diabetic monitoring applications.

Effects of Humidity on Foil and Vial Packaging to Preserve Glucose and Lactate Test Strips for Disaster Readiness

2014 ◽  
Vol 8 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Anh-Thu Truong ◽  
Richard F. Louie ◽  
John H. Vy ◽  
Corbin M. Curtis ◽  
William J. Ferguson ◽  
...  
Keyword(s):  
Disaster Response ◽  
Point Of Care ◽  
Test Strip ◽  
Rank Test ◽  
Test Strips ◽  
Signed Rank ◽  
Test Concentration ◽  
Signed Rank Test ◽  
Glucose Test ◽  
Experimental Trials

AbstractObjectiveEfficient emergency and disaster response is challenged by environmental conditions exceeding test reagent storage and operating specifications. We assessed the effectiveness of vial and foil packaging in preserving point-of-care (POC) glucose and lactate test strip performance in humid conditions.MethodsGlucose and lactate test strips in both packaging were exposed to mean relative humidity of 97.0 ± 1.1% in an environmental chamber for up to 168 hours. At defined time points, stressed strips were removed and tested in pairs with unstressed strips using whole blood samples spiked to glucose concentrations of 60, 100, and 250 mg/dL (n = 20 paired measurements per level). A Wilcoxon signed rank test was used to compare stressed and unstressed test strip measurements.ResultsStressed glucose and lactate test strip measurements differed significantly from unstressed strips, and were inconsistent between experimental trials. Median glucose paired difference was as high as 12.5 mg/dL at the high glucose test concentration. Median lactate bias was −0.2 mmol/L. Stressed strips from vial (3) and foil (7) packaging failed to produce results.ConclusionsBoth packaging designs appeared to protect glucose and lactate test strips for at least 1 week of high humidity stress. Documented strip failures revealed the need for improved manufacturing process. (Disaster Med Public Health Preparedness. 2014;0:1–7)

Effects of Dynamic Temperature and Humidity Stresses on Point-of-Care Glucose Testing for Disaster Care

2012 ◽  
Vol 6 (3) ◽  
pp. 232-240 ◽  
Author(s):  
Richard F. Louie ◽  
William J. Ferguson ◽  
Stephanie L. Sumner ◽  
Jimmy N. Yu ◽  
Corbin M. Curtis ◽  
...  
Keyword(s):  
Hurricane Katrina ◽  
Point Of Care ◽  
Glucose Dehydrogenase ◽  
Test Strips ◽  
Dynamic Temperature ◽  
Glucose Testing ◽  
Temperature And Humidity ◽  
Level 3 ◽  
Glucose Test ◽  
Level 1

ABSTRACTObjective: To characterize the performance of glucose meter test strips using simulated dynamic temperature and humidity disaster conditions.Methods: Glucose oxidase- and glucose dehydrogenase-based test strips were dynamically stressed for up to 680 hours using an environmental chamber to simulate conditions during Hurricane Katrina. Paired measurements vs control were obtained using 3 aqueous reagent levels for GMS1 and 2 for GMS2.Results: Stress affected the performance of GMS1 at level 1 (P <. 01); and GMS2 at both levels (P <. 001), lowering GMS1 results but elevating GMS2 results. Glucose median-paired differences were elevated at both levels on GMS2 after 72 hours. Median-paired differences (stress minus control) were as much as −10 mg/dL (range, −65 to 33) at level 3 with GMS1, with errors as large as 21.9%. Glucose median-paired differences were as high as 5 mg/dL (range, −1 to 10) for level 1 on GMS2, with absolute errors up to 24.4%.Conclusions: The duration of dynamic stress affected the performance of both GMS1 and GMS2 glucose test strips. Therefore, proper monitoring, handling, and storage of point-of-care (POC) reagents are needed to ensure their integrity and quality of actionable results, thereby minimizing treatment errors in emergency and disaster settings.(Disaster Med Public Health Preparedness. 2012;6:232–240)

A cucurbit[6]uril-based supramolecular assembly test strip for immediate detection of nitrofuran antibiotics in water

CrystEngComm ◽  
2020 ◽  
Vol 22 (44) ◽  
pp. 7660-7667
Author(s):  
Lulu Shi ◽  
Mei Liu ◽  
Cao Fang ◽  
Xiaofei Zhu ◽  
Hui Li
Keyword(s):  
Supramolecular Assembly ◽  
Test Strip ◽  
Test Strips ◽  
Naked Eye ◽  
Nitrofuran Antibiotics

A CB[6]-based supramolecular assembly has been designed and prepared into test strips, which can detect nitrofuran antibiotics by the naked eye.

scholarly journals The Influence of Blood Glucose Meter Resistance Variation on the Performance of a Biosensor with a Gold-Coated Circuit Board

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Kun-Yong Kim ◽  
Ho Chang ◽  
Win-Der Lee ◽  
Yi-Fan Cai ◽  
You-Jia Chen
Keyword(s):  
Blood Glucose ◽  
Glucose Oxidase ◽  
Economies Of Scale ◽  
Electrochemical Reaction ◽  
Test Strip ◽  
Circuit Board ◽  
Potential Range ◽  
Test Strips ◽  
Glucose Meter ◽  
Blood Glucose Meter

In this study, a novel gold-coated test strip for blood glucose measurement has been designed. Such gold-coated test strip is feasible for mass production to achieve economies of scale. Cyclic voltammetry was applied to test strips to undergo electrochemical reaction under a potential range of ±0.4 V. Glucose oxidase (GOD) was added into K3[Fe(CN)6]. When glucose oxidase undergoes electrochemical reaction, the medium, K3[Fe(CN)6], will act as an electron acceptor, causing the electrodes on the test strip to generate a pair of clear anodic and reductive peaks. The maximum of the anodic and reductive peaks can be used as reference to adjust the resistance of the blood glucose meter. The experimental results show that by adjusting the resistance of the blood glucose meter, the accuracy of blood glucose meter reading can be tuned and blood glucose reading can be stabilized. Therefore, when the resistance of the blood glucose meter is at 2.4 KΩ, the standard deviation (STD) and coefficient of variation (CV) of the test strip are lower than those of the test strips measured at resistances of 2.2 KΩ and 2.6 KΩ. It has been proved in this study that adjusting the resistance of the blood glucose meter can optimize the chemical reaction on gold-coated test strips as well as its reading. This method can also be applied to tune the accuracy of readings for test strips coated with other materials.

Cheating With Glucose Test Strips

Diabetes Care ◽  
1990 ◽  
Vol 13 (10) ◽  
pp. 1092-1093
Author(s):  
D. L. Horwitz
Keyword(s):  
Test Strips ◽  
Glucose Test ◽  
Glucose Test Strips
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