scholarly journals Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

2015 ◽  
Vol 51 (23) ◽  
pp. 4869-4871 ◽  
Author(s):  
Amnon Bar-Shir ◽  
Yajie Liang ◽  
Kannie W. Y. Chan ◽  
Assaf A. Gilad ◽  
Jeff W. M. Bulte
Keyword(s):  
Fluorescent Proteins ◽  
Chemical Exchange ◽  
Reporter Genes ◽  
Chemical Exchange Saturation Transfer ◽  
Green Fluorescent Proteins ◽  
Saturation Transfer ◽  
Wild Type ◽  
Mri Contrast ◽  
Cest Mri ◽  
Green Fluorescent

Superpositively charged GFP mutants demonstrate dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts.

scholarly journals CEST MRI provides amide/amine surrogate biomarkers for treatment-naïve glioma sub-typing

2022 ◽  
Author(s):  
Laura Mancini ◽  
Stefano Casagranda ◽  
Guillaume Gautier ◽  
Philippe Peter ◽  
Bruno Lopez ◽  
...  
Keyword(s):  
Chemical Exchange ◽  
Magnetization Transfer ◽  
Chemical Exchange Saturation Transfer ◽  
Magnetization Transfer Ratio ◽  
Saturation Transfer ◽  
Wild Type ◽  
Clinical Value ◽  
Cest Mri ◽  
Treatment Naïve ◽  
Glioma Classification

Abstract Purpose Accurate glioma classification affects patient management and is challenging on non- or low-enhancing gliomas. This study investigated the clinical value of different chemical exchange saturation transfer (CEST) metrics for glioma classification and assessed the diagnostic effect of the presence of abundant fluid in glioma subpopulations. Methods Forty-five treatment-naïve glioma patients with known isocitrate dehydrogenase (IDH) mutation and 1p/19q codeletion status received CEST MRI (B1rms = 2μT, Tsat = 3.5 s) at 3 T. Magnetization transfer ratio asymmetry and CEST metrics (amides: offset range 3–4 ppm, amines: 1.5–2.5 ppm, amide/amine ratio) were calculated with two models: ‘asymmetry-based’ (AB) and ‘fluid-suppressed’ (FS). The presence of T2/FLAIR mismatch was noted. Results IDH-wild type had higher amide/amine ratio than IDH-mutant_1p/19qcodel (p < 0.022). Amide/amine ratio and amine levels differentiated IDH-wild type from IDH-mutant (p < 0.0045) and from IDH-mutant_1p/19qret (p < 0.021). IDH-mutant_1p/19qret had higher amides and amines than IDH-mutant_1p/19qcodel (p < 0.035). IDH-mutant_1p/19qret with AB/FS mismatch had higher amines than IDH-mutant_1p/19qret without AB/FS mismatch ( < 0.016). In IDH-mutant_1p/19qret, the presence of AB/FS mismatch was closely related to the presence of T2/FLAIR mismatch (p = 0.014). Conclusions CEST-derived biomarkers for amides, amines, and their ratio can help with histomolecular staging in gliomas without intense contrast enhancement. T2/FLAIR mismatch is reflected in the presence of AB/FS CEST mismatch. The AB/FS CEST mismatch identifies glioma subgroups that may have prognostic and clinical relevance.

scholarly journals A generalized ratiometric chemical exchange saturation transfer (CEST) MRI approach for mapping renal pH using iopamidol

2017 ◽  
Vol 79 (3) ◽  
pp. 1553-1558 ◽  
Author(s):  
Yin Wu ◽  
Iris Y. Zhou ◽  
Takahiro Igarashi ◽  
Dario L. Longo ◽  
Silvio Aime ◽  
...  
Keyword(s):  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Cest Mri

Application of chemical exchange saturation transfer (CEST) MRI in acute stroke to visualize tissue acidosis and infarction risk

2013 ◽  
Vol 333 ◽  
pp. e205
Author(s):  
J. Blicher ◽  
A. Tietze ◽  
I.K. Mikkelsen ◽  
L. Østergaard ◽  
M. Strother ◽  
...  
Keyword(s):  
Acute Stroke ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Cest Mri ◽  
Tissue Acidosis

Tetrakis-(N-methyl-4-pyridinium)-porphyrin as a diamagnetic chemical exchange saturation transfer (diaCEST) MRI contrast agent

2021 ◽  
Author(s):  
Subhayan Chakraborty ◽  
Mainak Das ◽  
A. Srinivasan ◽  
Arindam Ghosh
Keyword(s):  
Contrast Agent ◽  
Chemical Exchange ◽  
Mri Contrast Agent ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Mri Contrast ◽  
Wide Range ◽  
Good Contrast

Easily synthesizable tetrakis-(N-methyl-4-pyridinium)-porphyrin as a diaCEST agent that shows nearly pH independent good contrast in a wide range of pH.

Lanthanide-Loaded Erythrocytes As Highly Sensitive Chemical Exchange Saturation Transfer MRI Contrast Agents

2013 ◽  
Vol 136 (2) ◽  
pp. 638-641 ◽  
Author(s):  
Giuseppe Ferrauto ◽  
Daniela Delli Castelli ◽  
Enza Di Gregorio ◽  
Sander Langereis ◽  
Dirk Burdinski ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Chemical Exchange ◽  
Mri Contrast Agents ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Mri Contrast ◽  
Highly Sensitive

Chemical exchange saturation transfer MRI contrast in the human brain at 9.4 T

NeuroImage ◽  
2018 ◽  
Vol 179 ◽  
pp. 144-155 ◽  
Author(s):  
Moritz Zaiss ◽  
Mark Schuppert ◽  
Anagha Deshmane ◽  
Kai Herz ◽  
Philipp Ehses ◽  
...  
Keyword(s):  
Human Brain ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Mri Contrast

scholarly journals Voxel-wise Optimization of Pseudo Voigt Profile (VOPVP) for Z-spectra fitting in chemical exchange saturation transfer (CEST) MRI

2019 ◽  
Vol 9 (10) ◽  
pp. 1714-1730 ◽  
Author(s):  
Lihong Zhang ◽  
Yingcheng Zhao ◽  
Yanrong Chen ◽  
Chongxue Bie ◽  
Yuhua Liang ◽  
...  
Keyword(s):  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Voigt Profile ◽  
Cest Mri ◽  
Fitting In ◽  
Spectra Fitting
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