Selective enrichment of proteins for MALDI-TOF MS analysis based on molecular imprinting

Lichee-like core–shell structured magnetic lutetium phosphate (Fe3O4@LuPO4) affinity microspheres were synthesized, characterized and successfully applied to enrich phosphopeptides.

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Magnetic metal–organic frameworks for selective enrichment and exclusion of proteins for MALDI-TOF MS analysis

The Analyst ◽

10.1039/c6an01335j ◽

2016 ◽

Vol 141 (15) ◽

pp. 4568-4572 ◽

Cited By ~ 12

Author(s):

Wei Wan ◽

Qionglin Liang ◽

Xiaoqiong Zhang ◽

Min Yan ◽

Mingyu Ding

Keyword(s):

Metal Organic Frameworks ◽

Maldi Tof Ms ◽

Selective Enrichment ◽

Maldi Tof ◽

Ms Analysis ◽

Magnetic Metal ◽

Metal Organic ◽

Tof Ms

We firstly report magnetic metal–organic frameworks for selective enrichment and exclusion of proteins for MALDI-TOF MS analysis.

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GC-MS and MALDI-TOF MS Profi ling of Sucrose Esters from Nicotiana tabacum and N. rustica

Zeitschrift für Naturforschung C ◽

10.1515/znc-2013-5-607 ◽

2013 ◽

Vol 68 (5-6) ◽

pp. 210-222

Author(s):

Łukasz P. Haliński ◽

Piotr Stepnowski

Keyword(s):

Sucrose Ester ◽

Sucrose Esters ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flame Ionization ◽

Flight Mass Spectrometry ◽

Ms Analysis ◽

Acyl Chains ◽

First Time ◽

Tof Ms

Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDITOF MS) has been applied for the first time to the analysis of the sucrose esters from the surface of Nicotiana L. leaves. The profiles obtained for the model plant N. tabacum were similar to those from the gas chromatography-flame ionization detector (GC-FID) analysis. The most reproducible results were obtained using a dihydroxybenzoic acid (DHB) matrix. The main advantage of this method is that crude plant extracts can be analysed without sample clean-up. GC-MS analysis of Aztec tobacco (N. rustica) extracts revealed the presence of three types of sucrose esters. All identified compounds had three C4 − C8 acyl chains substituting the glucose moiety, while the fructose part of the molecule was substituted with 0, 1, or 2 acetyl groups. MALDI-TOF MS analysis of the sucrose ester fraction revealed the presence of compounds not eluting from a GC column. Combining the data from both GC-MS and MALDI-TOF MS experiments, we obtained a full sucrose ester profile, which is based on the molecular weight of the compounds and on the number of acyl chains in the molecule.

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Highly efficient and selective enrichment of phosphopeptides using porous anodic alumina membrane for MALDI-TOF MS analysis

Journal of the American Society for Mass Spectrometry ◽

10.1016/j.jasms.2007.04.014 ◽

2007 ◽

Vol 18 (8) ◽

pp. 1387-1395 ◽

Cited By ~ 29

Author(s):

Yuebo Wang ◽

Wei Chen ◽

Jianshuang Wu ◽

Yinlong Guo ◽

Xinghua Xia

Keyword(s):

Anodic Alumina ◽

Porous Anodic Alumina ◽

Anodic Alumina Membrane ◽

Alumina Membrane ◽

Maldi Tof Ms ◽

Selective Enrichment ◽

Highly Efficient ◽

Maldi Tof ◽

Ms Analysis ◽

Tof Ms

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Selective separation and identification of metabolite groups of Polygonum cuspidatum extract in rat plasma using dispersion solid-phase extraction by magnetic molecularly imprinted polymers coupled with LC/Q-TOF-MS

RSC Advances ◽

10.1039/c5ra26695e ◽

2016 ◽

Vol 6 (15) ◽

pp. 12193-12204 ◽

Cited By ~ 16

Author(s):

Zaiyue Yang ◽

Qizhi Cai ◽

Ning Chen ◽

Xuemin Zhou ◽

Junli Hong

Keyword(s):

Molecularly Imprinted Polymers ◽

Solid Phase ◽

Rat Plasma ◽

Phase Extraction ◽

Polygonum Cuspidatum ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Magnetic Molecularly Imprinted Polymers ◽

Selective Enrichment ◽

Tof Ms

In this work, magnetic molecularly imprinted polymers (MMIPs) were successfully prepared for specific recognition and selective enrichment of metabolite groups of Polygonum cuspidatum extract in rat plasma.

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