A green and sustainable approach to utilize bio-ionic liquids for the selective precipitation of high purity agarose from an agarophyte extract

2015 ◽  
Vol 17 (5) ◽  
pp. 2867-2873 ◽  
Author(s):  
Mukesh Sharma ◽  
Jai Prakash Chaudhary ◽  
Dibyendu Mondal ◽  
Ramavatar Meena ◽  
Kamalesh Prasad

Preferential precipitation of agarose was observed upon addition of choline based bio-ionic liquids in the Gracilaria dura seaweed extract. Choline laurate was found to be the most effective for the work.

2014 ◽  
Vol 53 (17) ◽  
pp. 6871-6880 ◽  
Author(s):  
Da-Niu Cai ◽  
Kuan Huang ◽  
Yong-Le Chen ◽  
Xing-Bang Hu ◽  
You-Ting Wu

Holzforschung ◽  
2011 ◽  
Vol 65 (4) ◽  
Author(s):  
Michael Schrems ◽  
Agnieszka Brandt ◽  
Tom Welton ◽  
Falk Liebner ◽  
Thomas Rosenau ◽  
...  

Abstract The present study provides insight into the dissolution behavior of renewable materials in ionic liquids. Beech, spruce and rye straw were dissolved in 1-ethyl-3-methylimidazolium acetate as the ionic liquid of choice, which is currently one of the most frequently used cation-anion combinations among ionic liquids for biomaterial processing. The dissolution was followed by selective precipitation of cellulose, hemicelluloses, and lignin. The obtained lignin was analyzed with Curie-point pyrolysis gas chromatography/mass spectrometry (Cu-Py-GC/MS) and the separated cellulose/hemicellulose fractions with gel permeation chromatography (GPC). Time dependence of the dissolution process was studied on rye straw, eucalyptus kraft pulp and beech sulfite pulp. The results show a changing dissolution profile over time, which is due to progressing degradation of the cellulose in the ionic liquid.


2016 ◽  
Vol 678 ◽  
pp. 403-409 ◽  
Author(s):  
S.S. Kalyan Kamal ◽  
P.K. Sahoo ◽  
J. Vimala ◽  
B. Shanker ◽  
P. Ghosal ◽  
...  

Author(s):  
Dionis Minev ◽  
Richard Guerra ◽  
Jocelyn Y Kishi ◽  
Cory Smith ◽  
Elisha Krieg ◽  
...  

Abstract There is increasing demand for single-stranded DNA (ssDNA) of lengths >200 nucleotides (nt) in synthetic biology, biological imaging and bionanotechnology. Existing methods to produce high-purity long ssDNA face limitations in scalability, complexity of protocol steps and/or yield. We present a rapid, high-yielding and user-friendly method for in vitro production of high-purity ssDNA with lengths up to at least seven kilobases. Polymerase chain reaction (PCR) with a forward primer bearing a methanol-responsive polymer generates a tagged amplicon that enables selective precipitation of the modified strand under denaturing conditions. We demonstrate that ssDNA is recoverable in ∼40–50 min (time after PCR) with >70% yield with respect to the input PCR amplicon, or up to 70 pmol per 100 μl PCR reaction. We demonstrate that the recovered ssDNA can be used for CRISPR/Cas9 homology directed repair in human cells, DNA-origami folding and fluorescent in-situ hybridization.


2019 ◽  
Vol 42 (9) ◽  
pp. 1757-1767 ◽  
Author(s):  
Xiaoyong Zhao ◽  
Ge Ma ◽  
Datong Wu ◽  
Pengfei Cai ◽  
Yuanjiang Pan

2011 ◽  
Vol 50 (9) ◽  
pp. 5175-5190 ◽  
Author(s):  
Luz Maria Chávez-Islas ◽  
Ruben Vásquez-Medrano ◽  
Antonio Flores-Tlacuahuac

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