Chitosan-based biopolysaccharide proton conductors for synaptic transistors on paper substrates

2014 ◽  
Vol 2 (31) ◽  
pp. 6249-6255 ◽  
Author(s):  
Guodong Wu ◽  
Jin Zhang ◽  
Xiang Wan ◽  
Yi Yang ◽  
Shuanghe Jiang
Keyword(s):  
Signal Processing ◽  
Proton Conductors ◽  
Excitatory Postsynaptic Current ◽  
Paired Pulse ◽  
Paired Pulse Facilitation ◽  
Synaptic Functions ◽  
Dynamic Filtering ◽  
Paper Substrates

The chitosan-based paper synaptic transistors were successfully used as artificial synapses for emulating biological synaptic functions, including excitatory postsynaptic current, paired-pulse facilitation, dynamic filtering and spatiotemporally correlated signal processing.

Paired-pulse facilitation in the dentate gyrus: a patch-clamp study in rat hippocampus in vitro

1994 ◽  
Vol 72 (1) ◽  
pp. 326-336 ◽  
Author(s):  
M. Andreasen ◽  
J. J. Hablitz
Keyword(s):  
Patch Clamp ◽  
Dentate Gyrus ◽  
Time Constant ◽  
Rise Time ◽  
Time Course ◽  
Nmda Antagonist ◽  
Paired Pulse ◽  
Stimulation Intensity ◽  
Paired Pulse Facilitation ◽  
Epsc Amplitude

1. Whole-cell patch-clamp recordings were used to study paired-pulse facilitation (PPF) of the lateral perforant path input to the dentate gyrus in thin hippocampal slices. 2. Orthodromic stimulation of the lateral perforant pathway evoked a excitatory postsynaptic current (EPSC) with a latency of 3.3 +/- 0.1 ms (mean +/- SE) that fluctuated in amplitude. The EPSC had a rise time (10-90%) of 2.79 +/- 0.06 ms (n = 35) and decayed with a single exponential time course with a time-constant of 9.14 +/- 0.24 ms (n = 35). No correlation was found between the amplitude of the EPSC and the rise time or decay time-constant. The non-N-methyl-D-aspartate (NMDA) antagonist 6-cyano-7-nitroquinoxaline-2,3-dione completely blocked the EPSC whereas the NMDA antagonist D-aminophosphonovaleric acid (APV) had modest effects. 3. When a test (T-)EPSC was preceded at an interval of 100 ms by a conditioning (C-)EPSC, a significant increase in the amplitude of the T-EPSC was seen in 38 out of 44 trials analyzed from a total of 27 granule cells. The average amount of PPF was 35.7 +/- 2.1%. There was no apparent correlation between the amount of PPF and the stimulation intensity or mean amplitude of the C-EPSC. The time course of the facilitated T-EPSC was not significantly different from that of the C-EPSC. 4. No correlation was found between the amplitude of the C-EPSC and that of the T-EPSC. Estimates of quantal content (mcv) were determined by calculating the ratio of the squared averaged EPSC amplitude (from 48 responses) to the variance of these responses (M2/sigma 2) whereas quantal amplitudes (qcv) were estimated by calculating the ratio of the response variance to average EPSC amplitude (sigma 2/M). PPF was found to be associated with an average increase in mcv of 64.8 +/- 7.2% (n = 38) whereas qcv was decreased by 12.1 +/- 3.8%. 5. The time course of PPF was studied by varying the interval between the C- and T-pulse from 10 to 400 ms while keeping the stimulation intensity constant. Maximal facilitation of the T-EPSC was obtained with interpulse intervals < or = 25 ms where the average facilitation amounted to approximately 70% (n = 6). The decline of facilitation was nearly exponential and was no longer evident with intervals > 350 ms.(ABSTRACT TRUNCATED AT 400 WORDS

scholarly journals Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies

Science ◽  
2019 ◽  
Vol 364 (6440) ◽  
pp. 593-597 ◽  
Author(s):  
Caleb J. Bashor ◽  
Nikit Patel ◽  
Sandeep Choubey ◽  
Ali Beyzavi ◽  
Jané Kondev ◽  
...  
Keyword(s):  
Signal Processing ◽  
Self Assembly ◽  
Design Principle ◽  
Complex Signal ◽  
Synthetic Gene Circuits ◽  
Cellular Decision Making ◽  
Eukaryotic Genes ◽  
Transcription Factor Complexes ◽  
Dynamic Filtering ◽  
Cooperative Assembly

Eukaryotic genes are regulated by multivalent transcription factor complexes. Through cooperative self-assembly, these complexes perform nonlinear regulatory operations involved in cellular decision-making and signal processing. In this study, we apply this design principle to synthetic networks, testing whether engineered cooperative assemblies can program nonlinear gene circuit behavior in yeast. Using a model-guided approach, we show that specifying the strength and number of assembly subunits enables predictive tuning between linear and nonlinear regulatory responses for single- and multi-input circuits. We demonstrate that assemblies can be adjusted to control circuit dynamics. We harness this capability to engineer circuits that perform dynamic filtering, enabling frequency-dependent decoding in cell populations. Programmable cooperative assembly provides a versatile way to tune the nonlinearity of network connections, markedly expanding the engineerable behaviors available to synthetic circuits.

scholarly journals Visualization of Oxytocin Release that Mediates Paired Pulse Facilitation in Hypothalamic Pathways to Brainstem Autonomic Neurons

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112138 ◽  
Author(s):  
Ramón A. Piñol ◽  
Heather Jameson ◽  
Anastas Popratiloff ◽  
Norman H. Lee ◽  
David Mendelowitz
Keyword(s):  
Paired Pulse ◽  
Paired Pulse Facilitation ◽  
Autonomic Neurons ◽  
Oxytocin Release

scholarly journals A fresh look at paired-pulse facilitation

2001 ◽  
Vol 2 (5) ◽  
pp. 307-307 ◽  
Author(s):  
Juan Carlos López
Keyword(s):  
Paired Pulse ◽  
Paired Pulse Facilitation
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