scholarly journals Raman micro spectroscopy for in vitro drug screening: subcellular localisation and interactions of doxorubicin

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 4212-4223 ◽  
Author(s):  
Z. Farhane ◽  
F. Bonnier ◽  
A. Casey ◽  
H. J. Byrne
Keyword(s):  
Molecular Level ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Subcellular Localisation ◽  
Chemotherapeutic Drug ◽  
Lung Cancer Cell ◽  
In Vitro Drug Screening ◽  
Subcellular Resolution ◽  
Localization And Tracking

Raman spectroscopy is used for the localization and tracking of chemotherapeutic drug, doxorubicin, in the intracellular environment of lung cancer cell line. Results show the potential of the technique to monitor the mechanisms of action and response on a molecular level, with subcellular resolution.

Synthesis and in vitro Evaluation of Dihydrobenzimidazole Thiopyranooxazinone Derivatives as a Potent Biological Agents

2020 ◽  
Vol 5 (2) ◽  
pp. 164-170
Author(s):  
Deepak P. Kardile ◽  
Mrunal K. Shirsat
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
Cancer Cell Line ◽  
Docking Studies ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Anticancer Activities ◽  
In Silico Molecular Docking

In the present study, dihydrobenzimidazole thiopyranooxazinone derivatives were efficiently synthesized, which were further characterized and authenticated by means of TLC and different spectral analysis such as IR and 1H NMR. The synthesized compounds DPK2d2 to DPK2d8 were screened for their in vitro antimicrobial, antitubercular and anticancer activities. The results showed that the titled compounds DPK3d1, DPK3d2 and DPK3d4 exhibited potent antimicrobial activity, shows a broadspectrum activity against Bacillus subtilis, Escherichia coli (antibacterial) and Aspergillus niger (antifungal) as compared to ciprofloxacin and fluconazole, respectively. Compounds DPK3d1, DPK3d3 and DPK3d5 exhibited potent antitubercular activities against Mycobacterium tuberculosis as compared to pyrazinamide, ciprofloxacin and streptomycin. Compounds DPK3d3, DPK3d4 and DPK3d5 showed highly potent cytotoxic activity against human lung cancer cell line (A549) as compared to adriamycin. In silico molecular docking studies shown that all the ligands highest binding affinity range -6.7 to -8.7 for selected 1CB4 PDB of superoxide dismutase, which recognized that ligands having antioxidant activity.

scholarly journals RETRACTED ARTICLE: Synthesis, characterization and in vitro studies of doxorubicin-loaded magnetic nanoparticles grafted to smart copolymers on A549 lung cancer cell line

2012 ◽  
Vol 10 (1) ◽  
Author(s):  
Abolfazl Akbarzadeh ◽  
Mohammad Samiei ◽  
Sang Woo Joo ◽  
Maryam Anzaby ◽  
Younes Hanifehpour ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Magnetic Nanoparticles ◽  
Cancer Cell Line ◽  
Covalent Bonding ◽  
Lung Cancer Cell Line ◽  
Magnetic Nanocomposites ◽  
Lung Cancer Cell ◽  
A549 Lung Cancer Cell ◽  
A549 Lung

Abstract Background The aim of present study was to develop the novel methods for chemical and physical modification of superparamagnetic iron oxide nanoparticles (SPIONs) with polymers via covalent bonding entrapment. These modified SPIONs were used for encapsulation of anticancer drug doxorubicin. Method At first approach silane–grafted magnetic nanoparticles was prepared and used as a template for polymerization of the N-isopropylacrylamide (NIPAAm) and methacrylic acid (MAA) via radical polymerization. This temperature/pH-sensitive copolymer was used for preparation of DOX–loaded magnetic nanocomposites. At second approach Vinyltriethoxysilane-grafted magnetic nanoparticles were used as a template to polymerize PNIPAAm-MAA in 1, 4 dioxan and methylene-bis-acrylamide (BIS) was used as a cross-linking agent. Chemical composition and magnetic properties of Dox–loaded magnetic hydrogel nanocomposites were analyzed by FT-IR, XRD, and VSM. Results The results demonstrate the feasibility of drug encapsulation of the magnetic nanoparticles with NIPAAm–MAA copolymer via covalent bonding. The key factors for the successful prepardtion of magnetic nanocomposites were the structure of copolymer (linear or cross-linked), concentration of copolymer and concentration of drug. The influence of pH and temperature on the release profile of doxorubicin was examined. The in vitro cytotoxicity test (MTT assay) of both magnetic DOx–loaded nanoparticles was examined. The in vitro tests showed that these systems are no toxicity and are biocompatible. Conclusion IC50 of DOx–loaded Fe3O4 nanoparticles on A549 lung cancer cell line showed that systems could be useful in treatment of lung cancer.

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