scholarly journals Reliable microspotting methodology for peptide-nucleic acid layers with high hybridization efficiency on gold SPR imaging chips

2015 ◽  
Vol 7 (15) ◽  
pp. 6077-6082 ◽  
Author(s):  
L. Simon ◽  
G. Lautner ◽  
R. E. Gyurcsányi
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gold Surfaces ◽  
Complementary Dna ◽  
Spr Imaging ◽  
Hybridization Efficiency ◽  
Dna Strand

Microspotting of HS-PNA strands prehybridized with a complementary DNA strand onto gold surfaces results in PNA layers with excellent hybridization efficiency.

Synthesis of Stable Peptide Nucleic Acid-Modified Gold Nanoparticles and their Assembly onto Gold Surfaces

2013 ◽  
Vol 52 (15) ◽  
pp. 4217-4220 ◽  
Author(s):  
Philipp Anstaett ◽  
Yuanhui Zheng ◽  
Thibaut Thai ◽  
Alison M. Funston ◽  
Udo Bach ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gold Surfaces

PNA–sugar conjugates as tools for the spatial screening of carbohydrate–lectin interactions

2011 ◽  
Vol 84 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Christian Scheibe ◽  
Oliver Seitz
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Peptide Nucleic Acid ◽  
Modular Approach ◽  
Complementary Dna ◽  
Double Helices ◽  
Biological Recognition ◽  
Dna Strands ◽  
Carbohydrate Ligands ◽  
Spatial Presentation

Multivalent carbohydrate–lectin interactions are essential for a multitude of biological recognition events. Much effort has been spent in the synthesis of potent multivalent scaffolds in order to mimic or inhibit biological carbohydrate–protein interactions. However, the defined spatial presentation of carbohydrates remained a challenging task. Peptide nucleic acid (PNA)- and DNA-based double helices are useful scaffolds that enable the controlled display of carbohydrate ligands in a modular approach. The hybridization of PNA-sugar conjugates with complementary DNA strands provides multivalent complexes with defined spatial presentation of carbohydrates, which facilitates the spatial screening of carbohydrate–lectin interactions with Ångström-scale precision.

Quenching of fluorescently labeled pyrrolidinyl peptide nucleic acid by oligodeoxyguanosine and its application in DNA sensing

2020 ◽  
Vol 18 (30) ◽  
pp. 5951-5962
Author(s):  
Chayan Charoenpakdee ◽  
Tirayut Vilaivan
Keyword(s):  
Nucleic Acid ◽  
Electrostatic Interaction ◽  
Peptide Nucleic Acid ◽  
Dna Sensing ◽  
Complementary Dna

Oligodeoxyguanosine effectively quenches the fluorescence of PNA probes via electrostatic interaction, and the signal is restored by the addition of complementary DNA targets.

Pyrene-labeled pyrrolidinyl peptide nucleic acid as a hybridization-responsive DNA probe: comparison between internal and terminal labeling

RSC Advances ◽  
2014 ◽  
Vol 4 (17) ◽  
pp. 8817-8827 ◽  
Author(s):  
Chalothorn Boonlua ◽  
Boonsong Ditmangklo ◽  
Nisanath Reenabthue ◽  
Chaturong Suparpprom ◽  
Nattawee Poomsuk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Dna Probe ◽  
Complementary Dna

Internally pyrene-labeled pyrrolidinyl PNA yields much larger fluorescence increase than terminally labeled PNA upon hybridization with complementary DNA.

scholarly journals Furan-PNA: a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA

2016 ◽  
Vol 52 (42) ◽  
pp. 6930-6933 ◽  
Author(s):  
A. Manicardi ◽  
E. Gyssels ◽  
R. Corradini ◽  
A. Madder
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Double Stranded Dna ◽  
Dna Strand ◽  
Interstrand Crosslinking

Furan-modified peptide nucleic acid (PNA) probes are able to crosslink to DNA strand after hybridization with complementary ssDNA or after stand displacement in dsDNA.

Synthesis of Stable Peptide Nucleic Acid-Modified Gold Nanoparticles and their Assembly onto Gold Surfaces

2013 ◽  
Vol 125 (15) ◽  
pp. 4311-4314 ◽  
Author(s):  
Philipp Anstaett ◽  
Yuanhui Zheng ◽  
Thibaut Thai ◽  
Alison M. Funston ◽  
Udo Bach ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gold Surfaces

scholarly journals Metal Ion Chelates as Surrogates of Nucleobases for the Recognition of Nucleic Acid Sequences: ThePd2+Complex of 2,6-Bis(3,5-dimethylpyrazol-1-yl)purine Riboside

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Sharmin Taherpour ◽  
Tuomas Lönnberg
Keyword(s):  
Nucleic Acid ◽  
Metal Ion ◽  
Ternary Complex ◽  
Spectroscopic Studies ◽  
Complementary Dna ◽  
Micromolar Concentration ◽  
Oligonucleotide Hybridization ◽  
Dna Strand ◽  
Cd Studies ◽  
Uv Melting

A 2,6-bis(3,5-dimethylpyrazol-1-yl)purine ribonucleoside has been prepared and incorporated as a conventionally protected phosphoramidite into a 9-mer 2′-O-methyl oligoribonucleotide. According to 1H NMR spectroscopic studies, this nucleoside forms with Pd2+and uridine a ternary complex that is stable at a micromolar concentration range. CD spectroscopic studies on oligonucleotide hybridization, in turn, suggest that the Pd2+chelate of this artificial nucleoside, when incorporated in a 2′-O-methyl-RNA oligomer, is able to recognize thymine within an otherwise complementary DNA strand. The duplex containing thymidine opposite to the artificial nucleoside turned out to be somewhat more resistant to heating than its counterpart containing 2′-deoxycytidine in place of thymidine, but only in the presence of Pd2+. According to UV-melting measurements, replacement of 2′-O-methyladenosine with the artificial nucleoside markedly enhances hybridization with a DNA target, irrespective of the identity of the opposite base and the presence of Pd2+. With the thymidine containing DNA target, theTmvalue is 2–4°C higher than with targets containing any other nucleoside opposite to the artificial nucleoside, but the dependence on Pd2+is much less clear than in the case of the CD studies.

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