Transiently responsive protein–polymer conjugates via a ‘grafting-from’ RAFT approach for intracellular co-delivery of proteins and immune-modulators

2015 ◽  
Vol 51 (73) ◽  
pp. 13972-13975 ◽  
Author(s):  
N. Vanparijs ◽  
R. De Coen ◽  
D. Laplace ◽  
B. Louage ◽  
S. Maji ◽  
...  
Keyword(s):  
Raft Polymerization ◽  
Immune Modulators ◽  
Polymer Conjugates ◽  
Protein Polymer ◽  
Grafting From ◽  
Response To Temperature

‘Grafting-from’ RAFT polymerization is used to synthesize protein–polymer conjugates that change from the soluble to the aggregated state in response to temperature, but become fully soluble by acid triggered hydrolysis.

Visible light induced fast synthesis of protein–polymer conjugates: controllable polymerization and protein activity

2014 ◽  
Vol 50 (49) ◽  
pp. 6506-6508 ◽  
Author(s):  
Xin Li ◽  
Lei Wang ◽  
Gaojian Chen ◽  
David M. Haddleton ◽  
Hong Chen
Keyword(s):  
Visible Light ◽  
Ambient Temperature ◽  
Raft Polymerization ◽  
Protein Activity ◽  
Polymer Conjugates ◽  
Protein Polymer ◽  
Novel Method ◽  
Fast Synthesis

Visible light induced fast and controllable RAFT polymerization from protein as a novel method for preparing protein–polymer conjugates at ambient temperature.

On the immunological properties of penicillins

1971 ◽  
Vol 179 (1057) ◽  
pp. 411-432 ◽  
Keyword(s):  
Proteolytic Enzyme ◽  
Continuous Process ◽  
Polymeric Materials ◽  
Small Peptides ◽  
Physiological Conditions ◽  
Polymer Conjugates ◽  
Protein Polymer ◽  
Penicillanic Acid ◽  
Insoluble Form ◽  
Polyvalent Antigens

Penicilloylated proteins which may be found as impurities in 6-amino-penicillanic acid can be exhaustively digested by pronase to yield amino acids and small peptides. This degradation converts the potent polyvalent antigens into a mixture of mostly monovalent haptens which are much less immunogenic and less capable of eliciting immune reactions in sensitized animals. In order to avoid the contamination of 6-aminopenicillanic acid with a proteolytic enzyme, pronase was converted into a water insoluble form by coupling it with bromoacetyl cellulose. This insoluble derivative of pronase retains its activity and broad specificity. It can be readily removed from the medium upon completion of the impurity degradation, to be used repeatedly in a continuous process. The immunological manifestations associated with penicillins are not completely abolished by removal or degradation of protein impurities. Another important cause for these manifestations appears to be polymeric materials which are formed in penicillins. Such polymeric materials were isolated from ampicillin and shown to be capable of binding spontaneously to proteins (e.g. bovine serum albumin). The protein-polymer conjugates, which are formed under physiological conditions (pH 7.4, 37 °C), were found to be immunogenic and to provoke the formation of polymer-specific antibodies.

scholarly journals Effect of Polymer Hydrophilicity and Molar Mass on the Properties of the Protein in Protein–Polymer Conjugates: The Case of PPEylated Myoglobin

2021 ◽  
Author(s):  
Chiara Pelosi ◽  
Celia Duce ◽  
Frederik R. Wurm ◽  
Maria R. Tinè
Keyword(s):  
Molar Mass ◽  
Polymer Conjugates ◽  
Protein Polymer

scholarly journals Crosslinking of Self-Assembled Protein–Polymer Conjugates with Divanillin

2020 ◽  
Author(s):  
Zihao Li ◽  
Yanyan Jiang ◽  
Kilian Wüst ◽  
Manuela Callari ◽  
Martina H. Stenzel
Keyword(s):  
Polymer Conjugates ◽  
Protein Polymer ◽  
Self Assembled

Aqueous Protein–Polymer Bioconjugation via Photoinduced RAFT Polymerization Using High Loading Heterogeneous Catalyst

2021 ◽  
Author(s):  
Ya Huang ◽  
Xue Li ◽  
Yu Chi Zhang ◽  
Zhiwei Shi ◽  
Lun Zeng ◽  
...  
Keyword(s):  
Heterogeneous Catalyst ◽  
Raft Polymerization ◽  
High Loading ◽  
Protein Polymer

PET-RAFT polymerization catalyzed by cadmium selenide quantum dots (QDs): Grafting-from QDs photocatalysts to make polymer nanocomposites

2020 ◽  
Vol 11 (5) ◽  
pp. 1018-1024 ◽  
Author(s):  
Yifan Zhu ◽  
Eilaf Egap
Keyword(s):  
Quantum Dots ◽  
Cadmium Selenide ◽  
Polymer Nanocomposites ◽  
Chain Transfer ◽  
Raft Polymerization ◽  
Cdse Qds ◽  
Reversible Addition ◽  
Cadmium Selenide Quantum Dots ◽  
Grafting From ◽  
Chain Transfer Polymerization

We report herein the first example of light-controlled radical reversible addition–fragmentation chain transfer polymerization facilitated by cadmium selenide quantum dots and the grafting-from CdSe QDs to create polymer-QDs nanocomposites.

Precision Conjugation: An Emerging Tool for Generating Protein‐Polymer Conjugates

2020 ◽  
Author(s):  
Xinyu Liu ◽  
Weiping Gao
Keyword(s):  
Polymer Conjugates ◽  
Protein Polymer

scholarly journals Synthetic approach to homodimeric protein–polymer conjugates

2009 ◽  
pp. 2148 ◽  
Author(s):  
Lei Tao ◽  
Catherine S. Kaddis ◽  
Rachel R. Ogorzalek Loo ◽  
Gregory N. Grover ◽  
Joseph A. Loo ◽  
...  
Keyword(s):  
Synthetic Approach ◽  
Polymer Conjugates ◽  
Protein Polymer
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