Quantification of lactoferrin in breast milk by ultra-high performance liquid chromatography-tandem mass spectrometry with isotopic dilution

RSC Advances ◽  
2016 ◽  
Vol 6 (15) ◽  
pp. 12280-12285 ◽  
Author(s):  
Xing Ke ◽  
Qi Chen ◽  
Xiaodong Pan ◽  
Jingshun Zhang ◽  
Weimin Mo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Breast Milk ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Isotopic Dilution ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

We developed a LC-MS/MS method for quantification of human lactoferrin in breast milk based on tryptic peptides and a synthetic isotopic peptide standard.

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