A portable chromium ion detection system based on a smartphone readout device

2016 ◽  
Vol 8 (38) ◽  
pp. 6877-6882 ◽  
Author(s):  
Shiting Yu ◽  
Wei Xiao ◽  
Qiangqiang Fu ◽  
Ze Wu ◽  
Cuize Yao ◽  
...  

The portable detection system based on a smartphone readout device is a potential tool for environmental monitoring.

2020 ◽  
Vol 43 (6) ◽  
pp. 481-487
Author(s):  
Hui-Gyeong Kim ◽  
◽  
Yong-Ho Hong ◽  
Young-Seok Jung ◽  
Jae-Hyun Kim ◽  
...  

Author(s):  
Bo YANG ◽  
zhengwang shi ◽  
Yuan Ma ◽  
Lijuan Wang ◽  
Liyan Cao ◽  
...  

African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study, a LAMP assay coupled with the CRISPR Cas12a system was established in one tube for the detection of the ASFV p72 gene. The single-strand DNA-fluorophore-quencher (ssDNA-FQ) reporters and CRISPR-derived RNA (crRNAs) were screened and selected for the CRISPR detection system. In combination with LAMP amplification assay, the detection limit for the LAMP-CRISPR assay can reach 7 copies/μl of p72 gene per reaction. Furthermore, this method displays no cross-reactivity with other porcine DNA or RNA viruses. The performance of the LAMP-CRISPR assay was compared with real-time qPCR tests for clinical samples, a good consistency between the LAMP-CRISPR assay and real-time qPCR was observed. In the current study, a LAMP coupled with the CRISPR detection method was developed. The method shed a light on the convenient, portable, low cost, highly sensitive and specific detection of ASFV, demonstrating a great application potential for monitoring on-site ASFV in the field.


2015 ◽  
Author(s):  
Li Zhu ◽  
Yinchao Zhang ◽  
Siying Chen ◽  
He Chen ◽  
Pan Guo ◽  
...  

2008 ◽  
Vol 129 (2) ◽  
pp. 734-740 ◽  
Author(s):  
Inhee Choi ◽  
Sung Koo Kang ◽  
Jeongjin Lee ◽  
Younghun Kim ◽  
Jongheop Yi

2012 ◽  
Vol 45 (6) ◽  
pp. 464-469 ◽  
Author(s):  
Nan Li ◽  
Hui Xu ◽  
Qingjiang Li ◽  
Yinan Wang ◽  
Jinling Xing ◽  
...  

2013 ◽  
Vol 309 ◽  
pp. 44-49 ◽  
Author(s):  
Ramesh C. Sharma ◽  
Deepak Kumar ◽  
Neha Bhardwaj ◽  
Saurabh Gupta ◽  
Hukum Chandra ◽  
...  

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