Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification

We report sensitive and direct detection of specific bacterial dsDNA sequences using (i) the zinc finger protein labelled with alkaline phosphatase and (ii) electrochemical measurement at the ITO electrode.

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Enhanced Cleavage of Double-Stranded DNA by Artificial Zinc-Finger Nuclease Sandwiched between Two Zinc-Finger Proteins

Biochemistry ◽

10.1021/bi801800k ◽

2008 ◽

Vol 47 (47) ◽

pp. 12257-12259 ◽

Cited By ~ 15

Author(s):

Yusuke Mineta ◽

Tomoyuki Okamoto ◽

Kosuke Takenaka ◽

Norio Doi ◽

Yasuhiro Aoyama ◽

...

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins ◽

Zinc Finger Nuclease ◽

Double Stranded Dna

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Faculty Opinions recommendation of Enhanced cleavage of double-stranded DNA by artificial zinc-finger nuclease sandwiched between two zinc-finger proteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1138885.595978 ◽

2008 ◽

Author(s):

Amy Barrios ◽

Divya Krishnamurthy

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins ◽

Zinc Finger Nuclease ◽

Double Stranded Dna

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Application of engineered zinc finger proteins immobilized on paramagnetic beads for multiplexed detection of pathogenic DNA

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.2106.06057 ◽

2021 ◽

Author(s):

Jiyoung Shim ◽

Langley Williams ◽

Dohyun Kim ◽

Kisung Ko ◽

Moon-Soo Kim

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins ◽

Multiplexed Detection ◽

Paramagnetic Beads

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Faculty Opinions recommendation of Expanding the DNA-recognition repertoire for zinc finger proteins beyond 20 amino acids.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1013240.189751 ◽

2003 ◽

Author(s):

Jennifer Cha

Keyword(s):

Amino Acids ◽

Zinc Finger ◽

Dna Recognition ◽

Zinc Finger Proteins

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Faculty Opinions recommendation of Reduction in DNA-binding affinity of Cys2His2 zinc finger proteins by linker phosphorylation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1019055.215578 ◽

2004 ◽

Author(s):

Philip Dawson

Keyword(s):

Dna Binding ◽

Binding Affinity ◽

Zinc Finger ◽

Zinc Finger Proteins ◽

Dna Binding Affinity

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Faculty Opinions recommendation of A family of zinc-finger proteins is required for chromosome-specific pairing and synapsis during meiosis in C. elegans.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1053788.506625 ◽

2006 ◽

Author(s):

Robert K Herman

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins ◽

C Elegans ◽

Specific Pairing

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An Elastic-Network-Based Local Molecular Field Analysis of Zinc Finger Proteins

The Journal of Physical Chemistry B ◽

10.1021/jp200244r ◽

2011 ◽

Vol 115 (22) ◽

pp. 7374-7382 ◽

Cited By ~ 6

Author(s):

Purushottam D. Dixit ◽

D. Asthagiri

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins ◽

Field Analysis ◽

Molecular Field ◽

Elastic Network ◽

Molecular Field Analysis

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Engineering Zinc Finger Proteins using Accessory Binding Modules

Biophysical Journal ◽

10.1016/j.bpj.2011.11.433 ◽

2012 ◽

Vol 102 (3) ◽

pp. 74a

Author(s):

Kathryn Trenshaw ◽

Younghoon Kim ◽

Nathan Yee ◽

Peiyi Wang ◽

Charles Schroeder

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins

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Identification of non-Ser/Thr-Pro consensus motifs for Cdk1 and their roles in mitotic regulation of C2H2 zinc finger proteins and Ect2

Scientific Reports ◽

10.1038/srep07929 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 32

Author(s):

Kazuhiro Suzuki ◽

Kosuke Sako ◽

Kazuhiro Akiyama ◽

Michitaka Isoda ◽

Chiharu Senoo ◽

...

Keyword(s):

Zinc Finger ◽

Zinc Finger Proteins ◽

C2h2 Zinc Finger ◽

Consensus Motifs ◽

Mitotic Regulation

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