Evaluating a novel dimensional reduction approach for mechanical fractionation of cells using a tandem flexible micro spring array (tFMSA)

Lab on a Chip ◽  
2017 ◽  
Vol 17 (4) ◽  
pp. 691-701 ◽  
Author(s):  
Yin-Ting Yeh ◽  
Ramdane A. Harouaka ◽  
Si-Yang Zheng
Keyword(s):  
Physical Properties ◽  
Dimensional Reduction ◽  
Rational Design ◽  
Experimental Models ◽  
Cell Fractionation ◽  
Reduction Approach

We present a novel methodology to establish experimental models for the rational design of cell fractionation based on physical properties of cells.

scholarly journals Experimental and theoretical explorations of nanocarriers’ multistep delivery performance for rational design and anticancer prediction

2021 ◽  
Vol 7 (6) ◽  
pp. eaba2458
Author(s):  
Weier Bao ◽  
Falin Tian ◽  
Chengliang Lyu ◽  
Bin Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Physical Properties ◽  
Rational Design ◽  
Theoretical Models ◽  
Cell Uptake ◽  
Anticancer Efficacy ◽  
Delivery Performance ◽  
Simulation Based ◽  
Multistep Process

The poor understanding of the complex multistep process taken by nanocarriers during the delivery process limits the delivery efficiencies and further hinders the translation of these systems into medicine. Here, we describe a series of six self-assembled nanocarrier types with systematically altered physical properties including size, shape, and rigidity, as well as both in vitro and in vivo analyses of their performance in blood circulation, tumor penetration, cancer cell uptake, and anticancer efficacy. We also developed both data and simulation-based models for understanding the influence of physical properties, both individually and considered together, on each delivery step and overall delivery process. Thus, beyond finding that nanocarriers that are simultaneously endowed with tubular shape, short length, and low rigidity outperformed the other types, we now have a suit of theoretical models that can predict how nanocarrier properties will individually and collectively perform in the multistep delivery of anticancer therapies.

scholarly journals GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITRO

1974 ◽  
Vol 140 (6) ◽  
pp. 1511-1521 ◽  
Author(s):  
H. Robson MacDonald ◽  
Jean-Charles Cerottini ◽  
K. Theodor Brunner
Keyword(s):  
T Lymphocytes ◽  
Physical Properties ◽  
Cell Lineage ◽  
Kinetic Studies ◽  
Large Cell ◽  
Velocity Sedimentation ◽  
Cell Fractionation ◽  
Anamnestic Response ◽  
Cell Fractions

Separation of cells by velocity sedimentation at unit gravity was utilized to investigate the physical properties of cytotoxic thymus-derived lymphocytes (CTL) generated in long-term mixed leukocyte cultures (MLC). In kinetic studies, CTL were found almost exclusively in the large cell fractions at the peak of the response on day 4, whereas the majority of CTL in day 14 MLC had the sedimentation properties of small lymphocytes. Reculture until day 14 of cells fractionated on the basis of size on day 4 indicated that the small CTL were derived exclusively from cells which had been large on day 4. Re-exposure of day 14 MLC cells to the original stimulating alloantigens resulted in significant cell proliferation and rapid regeneration of CTL activity. Cell fractionation experiments demonstrated that the cells in the day 14 MLC population which responded to the secondary allogeneic stimulus were small T lymphocytes, and that these cells rapidly developed into large, highly cytotoxic CTL following stimulation. Moreover, by restimulating on day 14 fractions which were selected on the basis of size on day 4, it was found that the responding small lymphocytes were themselves the progeny of cells which were large at the peak of the response. Since CTL and CTL progenitors showed concomitant changes in physical properties with time, the possibility exists that they belong to the same cell lineage, and hence that CTL can differentiate into cells which are no longer cytotoxic, but capable of mounting an anamnestic response.

Additional Article Notification: Rational design and applications of conducting polymer hydrogels as electrochemical biosensors

2015 ◽  
Vol 3 (25) ◽  
pp. 5111-5121 ◽  
Author(s):  
Lanlan Li ◽  
Ye Shi ◽  
Lijia Pan ◽  
Yi Shi ◽  
Guihua Yu
Keyword(s):  
Physical Properties ◽  
Water Content ◽  
Conducting Polymer ◽  
Rational Design ◽  
High Water ◽  
High Water Content ◽  
Electrochemical Biosensors ◽  
Polymer Hydrogels ◽  
Extracellular Environment

Conducting polymer hydrogels (CPHs) are conducting polymer-based materials that contain high water content and have physical properties, resembling the extracellular environment.

A dimensional reduction approach to modulate the core ruminal microbiome associated with methane emissions via selective breeding

2021 ◽  
Author(s):  
Alejandro Saborío-Montero ◽  
Adrían López-García ◽  
Mónica Gutiérrez-Rivas ◽  
Raquel Atxaerandio ◽  
Idoia Goiri ◽  
...  
Keyword(s):  
Dimensional Reduction ◽  
Selective Breeding ◽  
Methane Emissions ◽  
The Core ◽  
Reduction Approach
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