Au nanocluster-embedded chitosan nanocapsules as labels for the ultrasensitive fluorescence immunoassay of Escherichia coli O157:H7

The Analyst ◽  
2018 ◽  
Vol 143 (17) ◽  
pp. 4067-4073 ◽  
Author(s):  
Chang Cheng ◽  
Lu Yang ◽  
Miao Zhong ◽  
Wenfang Deng ◽  
Yueming Tan ◽  
...  

Ultrasensitive fluorescence immunoassay of Escherichia coli O157:H7 is described using Au nanocluster-embedded chitosan nanocapsules as labels.

2006 ◽  
Vol 148 (6) ◽  
pp. 289-295 ◽  
Author(s):  
C. Zweifel ◽  
M. Kaufmann ◽  
J. Blanco ◽  
R. Stephan

2020 ◽  
Vol 23 (3) ◽  
pp. 310-318
Author(s):  
K. Koev ◽  
T. Stoyanchev ◽  
G. Zhelev ◽  
P. Marutsov ◽  
K. Gospodinova ◽  
...  

The purpose of this study was to detect the presence of shiga-toxin producing Escherichia coli (STEC) in faeces of healthy dairy cattle and to determine the sensitivity of isolates to several anti­microbial drugs. A total of 1,104 anal swab samples originating from 28 cattle farms were examined. After the primary identification, 30 strains were found to belong to serogroup О157. By means of conventional multiplex PCR, isolates were screened for presence of resistance genes stx1, stx2 and eaeА. Twenty-nine strains possesses amplicons with a size corresponding to genes stx2 and eaeA, one had amplicons also for the stx1 gene and one lacked amplicons of all three genes. Twenty-eight strains demonstrated amplicons equivalent to gene H7. The results from phenotype analysis of resistance showed preserved sensitivity to ceftriaxone, ceftazidime, cefotaxime, cephalothin, streptomycin, gentamicin, tetracycline, enrofloxacin and combinations sulfamethoxazole/trimethoprim and amoxicillin/clavulanic acid. Sensitivity to ampicillin was relatively preserved, although at a lower extent.


2017 ◽  
Vol 24 (2) ◽  
pp. 181-187 ◽  
Author(s):  
Yinliang Ma ◽  
Guohui Bai ◽  
Yaqi Cui ◽  
Jing Zhao ◽  
Zenglin Yuan ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document