Sensitivity of vaginal and mammary bacterial microflora of sows from patients with postpartum dysgalactic syndrome to antibacterial drugs

Purpose: Investigation of the sensitivity of conditionally pathogenic and pathogenic causative agents of postpartum disgalacting syndrome isolated from the pathological discharge of the vagina and the secretion of the mammary glands of patients with sorts to the most common antibacterial drugs.Materials and methods. The fence of the biological material was carried out within 2-3 days after supporting in sows of different ages and parity with the clinical manifestation of postpartum disgalacting syndrome from June to August 2021. In sows, the purse was taken in a mosper, milk, discharge from the vagina for the purpose of bacteriological research.Samples of vaginal wasches were sent in a special transportation environment of Ames. Samples of colostrum and milk (3-5 ml) were gained in sterile test tubes in compliance with the rules of antiseptics (the mammary glands were laid with warm water and 70% ethyl alcohol were treated). For the allocation and study of pure cultures of microorganisms from the above biomaterials produced crops on various nutrient media.The material brought to the laboratory was studied as follows: from the transport medium was carried out primary sowing on triptica-soybean agar, tryptichase-soybean broth, triptichase-soy agar with the addition of 5% of the defibrous blood of the ram; Samples were incubated in aerobic conditions at 37; The growth was taken into account after 24 hours. Then, pure cultures were isolated for the study of the cultural and morphological properties of the microorganisms obtained.The primary identification of the strains of microorganisms was carried out using the Microflex® LRF Bruker Maldi Biotyper system. The accuracy of the results obtained was confirmed by classical microbiological methods based on morphological, cultural and biochemical signs of microorganisms.The resistance of the isolated and identified pure crops to antibiotics was determined by diffusion in agar. In the study, the "advanced set of disks to estimate the antibiotic sensitivity of enterobacteria was used.Results. Inflammatory processes in the reproductive path of sows with postpartum diskalactic syndrome are caused by predominant gram-negative microorganisms, in lactic glands - associations of gram-positive and gram-negative microflora. The main conditionally pathogenic and pathogenic pathogens of endometritis and / or mastitis associated with postpartum diskalactic syndrome are the microorganisms of Escherichia, Enterobacter, Klebsiella, Actinobacillus, Rothia, Weisella, Pseudomonas, Proteus, Enterococcus, Streptococcus and Staphylococcus. It has been established that the overwhelming majority of microorganisms are sensitive to cephalosporine antibiotics: cefepim, zefisim, cefotaxim, ceftazidim, ceftriaxone, ceftinibuthen, cefuroxime.Conclusion. With respect to a large number of the most frequently used antibacterial drugs on this pig-breeding enterprise, high resistance is observed in microorganisms. Treatment of postpartum diskalactic syndrome sowers using antibacterial drugs is recommended to be carried out taking into account sensitivity to them allocated conditional and pathogenic microorganisms.

1692 No Longer Unknown: A Systematic Review & Meta-Analysis of The Effectiveness of Trans-Oral Surgical Techniques in Identifying Head and Neck Primary Cancer in Carcinoma Unknown Primary

Aim The use of transoral robotic surgery (TORS), transoral laser microsurgery (TLM) and more recently reported transoral endoscopic electrocautery (TOEC) in the identification of the primary cancer in CUP patients has gained popularity. This systematic review aims to assess the effectiveness of TORS, TLM and TOEC. Method A systematic review and meta-analysis using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting methodology was carried out to assess the effectiveness of the three trans-oral surgical techniques. EMBASE, MEDLINE and CINAHL databases were searched from inception to September 2020. Primary outcome measure was detection rates of primary cancer of the different techniques. Secondary outcome measures were complications and length of hospital stay. Results 289 studies were identified of which 30 met the inclusion criteria (28 case series and 2 case reports). The overall combined primary identification rate was 72.3% (567 /777 patients). The primary identification rates were 49.7% and 34.2% in lingual (n = 273) and palatine tonsillectomy (n = 118) respectively. The primary cancer identification rates by surgical techniques are: TORS was 60% (CI 0.49, 0.70), TLM was 80% (CI 0.58, 1.01), TOEC was 41% (CI 0.05, 0.76). The commonest complication was haemorrhage (5.3%). Conclusions This is the largest systematic review in the subject and incorporates the more recently published surgical technique of TOEC. Lingual tonsillectomy is an effective procedure in CUP work up. Further larger, multi-centre, prospective studies of PET CT negative CUP patients is needed to draw conclusive results

Molecular Exploring of Plasmid-mediated Ampc beta Lactamase Gene in Clinical Isolates of Proteus mirabilis

Between September to December 2020, thirteen isolates of Proteus mirabilis were recovered among one hundred fifty; MacConkey agar was utilized to purify Gram-negative bacteria isolated from infections of the urinary tract. The primary identification of Proteus mirabilis isolates was relied on “colonial morphology, microscopic examination, and biochemical “tests; however, the confirmation of identification of antimicrobial susceptibility of isolates was conducted utilizing an automated VITEK-2 compact system. The result showed that Proteus mirabilis isolates were highly resistant to most antibiotics, making them multi-drug resistant (MDR). Phenotype methods were used to detect AmpC beta-lactamase. Initial and confirmatory methods showed that eight isolates were AmpC producers. Polymerase Chain Reaction (PCR) was employed to detect the blaampC gene.

Management of The Dead and Missing People in the Easter Bombings in Colombo, Sri Lanka

Identification of the victims is considered as one of the most important initial steps in the management of a mass disaster. Comparison of ante-mortem and post-mortem fingerprints (ridgeology), dental data and DNA profiles have been recognized as primary identification methods for Disaster Victim Identification (DVI). However, facial recognition and personal belongings are the widely used tools of identification in large disasters. A series of bombings hit Sri Lanka on the morning of 21 st of April 2019. In the city of Colombo around 131 people died. Most of the identifications were achieved through visual recognition, with a minor percentage by odontology, genetics and fingerprints. The procedure adopted in the response to the disaster is described in this paper highlighting the importance of advanced preparedness, inter-institutional cooperation, the empathetic approach in caring for the grieving families and the procedure to adopt in visual recognition in DVI.

First Report of Multi-drug Resistant Staphylococcus haemolyticus in Nosocomial Infections in North Western Saudi Arabia

We report in this study for the first time the prevalence of multiple resistant Staphylococcus haemolyticus in clinical settings in Saudi Arabia. A total of 1060 clinical specimens of hospitalized patients were screened for the presence of S. haemolyticus in the period between September and December 2020. Primary identification of the isolates was carried out by colonial characteristics on mannitol salt agar and clumping factor test, confirmation of presumptive isolates and antimicrobial susceptibility testing was performed by Vitek® 2, while PCR was employed to detect mecA and vanA genes. A total of 20 S. haemolyticus isolates were recovered from 20 samples (blood cultures, urine, nasal swab, wound swab, groin swab, and axilla swab), 90% (P <0.001, x2) of the isolates were multiple resistant to three antimicrobial agents and more. Resistance to oxacillin was exhibited in 95% of the isolates, while none of the isolates were resistant to vancomycin and linezolid, yet resistance to rifampicin was observed in 30 % of the isolates. The findings of this study highlights the emerging trends of Staphylococcus haemolyticus as potential drug resistant pathogen in hospital settings in Saudi Arabia, which requires in depth investigation on molecular understanding on antimicrobial resistance and virulence traits of the strains.

The use of the forest management database in the process of primary selection of stands within forest genetic resources

The forest management database is used for the primary selection of the candidate stands for inclusion in forest genetic resources (FGR). The management and primary processing of the forest management database was performed with the support of standard functions of commonly used software (Microsoft Excel). In 2020, the primary identification area of FGR was 75.7 thousand ha of land covered with forests in the central area of the Republic of Moldova. As a result of the data processing, 12.7 thousand ha, or 16.8% of the study area, of candidate stands were selected for inclusion in the FGR.

Anomali gigi sebagai sarana identifikasi forensik

Objectives: The identification process is very important to determine the identity of the corpse. Identification through teeth is a primary identification method that is often used because teeth have a high degree of individuality. Identification through teeth can be analyzed through dental anomalies. Dental anomalies can assist in the identification process because of their uniqueness, stability, and high incidence in different populations. This paper aims to provide information on the importance of dental anomalies as a relevant and scientific means for identification purposes. Review: Dental anomalies can be defined as morphological abnormalities of the teeth that occur at the stage of growth and development of teeth. Dental anomalies are also inherited disorders that can occur as part of a syndrome. The wide variety of dental abnormalities in the form of size, number, morphology, structure or position of teeth in the oral cavity that can be possessed by each individual can be a characteristic of that individual. Conclusion: The use of dental anomalies in identification has been scientifically recognized with proper diagnosis and writing of dental anomalies in the medical record can be used for identification purposes in the forensic field.

Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva

A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary identification of isolates was carried out by amplification and MvaI restriction fragment length polymorphism (RFLP) of the internal transcribed spacers regions of ribosomal DNA (ITS-rDNA). The identifications, especially in the cases of isolates with unknown RFLP patterns, were confirmed by sequencing of the ITS-rDNA region. As a result, 256 isolates were recovered. The isolation rate was higher in soils with pH range 7.1–8.0, collected from animal habitats (n = 78; 34%) and parks and gardens (n = 75; 32%), geographically from Mazandaran Province (n = 115; 49.5%) and seasonally in the spring (n = 129; 50.4%), all of which were statistically significant (p < 0.05). The dermatophytes comprising five species of the two genera, viz., Nannizzia fulva (n = 214), N. gypsea (n = 34), Arthroderma quadrifidum (n = 5), A. gertleri (n = 2) and A. tuberculatum (n = 1), were isolated. The geophilic dermatophytes occurred in various soils from different parts of Iran; however, surprisingly, N. fulva emerged as the dominant species, outnumbering the common geophilic species of N. gypsea. For the definitive identification of soil inhabitant dermatophytes, DNA-based identification is strongly recommended.

Between City, King, and Empire: Will the Real “Lady of Byblos” Please Stand Up?

Who was the goddess known anciently as the “Lady of Byblos”? Typically, scholars have tried to answer this question by identifying the goddess’s “true” proper name. By contrast, this article emphasizes the goddess’s primary identification by the city of Byblos as a social-political community in order to analyze the Lady of Byblos’s role in shaping Late Bronze Age Byblos’s political landscape, which included imperial, royal, and collective modes of governance. The goddess’s place in Byblos’s political-religious economy thus serves as a fruitful case study for better conceptualizing through the lens of religion the complex range of potential interactions in the ancient world between centralizing and decentralizing political forces as parts of a single social-political system. In this way, the Lady of Byblos may “stand up” not only as an integral member of Byblos’s social order and religious life, but also as an example of the fundamental role that deities played in shaping ancient political realities.

Phenotypic Characterization, Genetic Analysis and Antibiotic Sensitivity of Aeromonas Hydrophila Isolates Causing Abdominal Dropsy in Labeo Rohita Collected From Punjab, Pakistan

Motile Aeromonas Septicaemia (MAS) is a common fresh water fish disease and major threat to the aquaculture in Pakistan. The present study was carried out on suspected cases of Rohu (Labeo rohita) to characterize and genetically analyze local strains of Aeromonas (A.) hydrophila, a key pathogen responsible for the said disease in farmed fish of Pakistan. A total of ninety suspected samples were collected from fish farms in various districts of Punjab from June 2018 to April 2019. The samples were processed and A. hydrophila strains were isolated. The primary identification of sixty seven isolates was verified by colony morphology, microscopy and phenotypic characterization with ten biochemical reactions. The A. hydrophila strains of test samples were characterized by polymerase chain reaction (PCR) using 16S rRNA at desired size of 356 bp. The PCR amplified product was subjected to DNA sequencing and phylogenetic analysis showed homology with related strains of Aeromonas spp. By antibiotic sensitivity test, the isolates were checked for nine antibiotics, in which pathogen was sensitive to four and resistant to five drugs. Results of molecular characterization confirmed strains as A. hydrophila which are useful to take preventive measures against the said disease.