Practical method for superresolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification

Expansion microscopy was applied to the observation of primary cilia and centrioles. To compensate for the decrease in fluorescence signal per unit volume inherent in physical expansion, a fluorescence signal amplification tool, called the amplibody, was developed. The method enables the practical observation of cilia and centrioles with high brightness and resolution.

A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

A DNA-based molecular machine triggered only by a specific target neuropeptide has been developed and integrated with fluorescence signal amplification system. It enabled rapid and sensitive detection of the target neuropeptide in biological matrices.

A signal amplifying fluorescent nanoprobe and lateral flow assay for ultrasensitive detection of cardiac biomarker troponin I

Novel functionalized nanoprobes based on a biotin–streptavidin system led to fluorescence signal amplification and the improvement of cTnI detection sensitivity.