scholarly journals Effect of pyrolysis on the removal of antibiotic resistance genes and class I integrons from municipal wastewater biosolids

2018 ◽  
Vol 4 (11) ◽  
pp. 1807-1818 ◽  
Author(s):  
Lee K. Kimbell ◽  
Anthony D. Kappell ◽  
Patrick J. McNamara
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Class I ◽  
Substantial Portion ◽  
Class I Integrons

Biosolids carry a substantial portion of antibiotic resistance genes (ARGs) leaving wastewater treatment plants. Pyrolysis substantially reduces ARGs in biosolids.

scholarly journals Occurrence of Fluoroquinolones and Sulfonamides Resistance Genes in Wastewater and Sludge at Different Stages of Wastewater Treatment: A Preliminary Case Study

2020 ◽  
Vol 10 (17) ◽  
pp. 5816
Author(s):  
Damian Rolbiecki ◽  
Monika Harnisz ◽  
Ewa Korzeniewska ◽  
Łukasz Jałowiecki ◽  
Grażyna Płaza
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Sewage Sludge ◽  
Resistance Genes ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Treated Wastewater ◽  
Standard Polymerase Chain Reaction ◽  
Wastewater Samples

This study identified differences in the prevalence of antibiotic resistance genes (ARGs) between wastewater treatment plants (WWTPs) processing different proportions of hospital and municipal wastewater as well as various types of industrial wastewater. The influence of treated effluents discharged from WWTPs on the receiving water bodies (rivers) was examined. Genomic DNA was isolated from environmental samples (river water, wastewater and sewage sludge). The presence of genes encoding resistance to sulfonamides (sul1, sul2) and fluoroquinolones (qepA, aac(6′)-Ib-cr) was determined by standard polymerase chain reaction (PCR). The effect of the sampling season (summer – June, fall – November) was analyzed. Treated wastewater and sewage sludge were significant reservoirs of antibiotic resistance and contained all of the examined ARGs. All wastewater samples contained sul1 and aac(6′)-lb-cr genes, while the qepA and sul2 genes occurred less frequently. These observations suggest that the prevalence of ARGs is determined by the type of processed wastewater. The Warmia and Mazury WWTP was characterized by higher levels of the sul2 gene, which could be attributed to the fact that this WWTP processes agricultural sewage containing animal waste. However, hospital wastewater appears to be the main source of the sul1 gene. The results of this study indicate that WWTPs are significant sources of ARGs, contributing to the spread of antibiotic resistance in rivers receiving processed wastewater.

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