Shotgun metagenomic analysis reveals the prevalence of antibiotic resistance genes and mobile genetic elements in full scale hospital wastewater treatment plants

2021 ◽  
Vol 296 ◽  
pp. 113270
Author(s):  
Ranjith Kumar Manoharan ◽  
Sathiyaraj Srinivasan ◽  
Gnanendra Shanmugam ◽  
Young-Ho Ahn
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Mobile Genetic Elements ◽  
Full Scale ◽  
Metagenomic Analysis ◽  
Hospital Wastewater ◽  
Genetic Elements

scholarly journals Antibiotic Resistance Genes Dynamics at the Different Stages of the Biological Process in a Full-Scale Wastewater Treatment Plant

Proceedings ◽  
2018 ◽  
Vol 2 (11) ◽  
pp. 650 ◽  
Author(s):  
Ioanna Zerva ◽  
Ioanna Alexandropoulou ◽  
Maria Panopoulou ◽  
Paraschos Melidis ◽  
Spyridon Ntougias
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plant ◽  
Biological Process ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Antibiotic Resistance Genes ◽  
Additional Treatment ◽  
Full Scale

Wastewater treatment plants (WWTPs) highly contribute to the transmission of antibiotic resistance genes (ARGs) in the environment. In this work, the diversity of ermF, ermB, sul1 and int1-enconding genes was examined in the influent, the mixed liquor and the effluent of a full-scale WWTP. Based on the clones analyzed, similar genotypes were recorded at all process stages. However, distinct genotypes of int1 were responsible for the expression of sul1 and ermF genes in Gammaproteobacteria and Bacteroidetes, respectively. Due to the detection of similar ARGs profiles throughout the biological process, it is concluded that additional treatment is needed for their retention.

scholarly journals Metagenomic Analysis Reveals the Fate of Antibiotic Resistance Genes in a Full-Scale Wastewater Treatment Plant in Egypt

2021 ◽  
Vol 13 (20) ◽  
pp. 11131
Author(s):  
Osama S. Ali ◽  
Walaa G. Hozayen ◽  
Abdulwahab S. Almutairi ◽  
Sherif A. Edris ◽  
Aala A. Abulfaraj ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Community Composition ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Full Scale ◽  
Metagenomic Analysis ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Treated Effluent

Wastewater treatment plants (WWTPs) are recognized as hotspots for the dissemination of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARBs) in the environment. Our study utilized a high-throughput sequencing-based metagenomic analysis approach to compare the ARG abundance profiles of the raw sewage, treated effluent and activated sludge samples from a full-scale WWTP in Egypt. In addition, the difference in microbial community composition due to the treatment process was assessed. As a result, 578 ARG subtypes (resistance genes) belonging to 18 ARG types (antibiotic resistance classes) were identified. ARGs encoding for resistance against multidrug, aminoglycoside, bacitracin, beta-lactam, sulfonamide, and tetracycline antibiotics were the most abundant types. The total removal efficiency percentage of ARGs in the WWTP was found to be 98% however, the ARG persistence results indicated that around 68% of the ARGs in the influent could be found in the treated effluent. This finding suggests that the treated wastewater poses a potential risk for the ARG dissemination in bacterial communities of the receiving water bodies via horizontal gene transfer (HGT). The community composition at phylum level showed that Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were the most abundant phyla in all datasets. Although the relative abundance of several pathogenic bacteria in the influent declined to less than 1% in the effluent, the taxonomic assignments at species level for the effluent and sludge metagenomes demonstrated that clinically important pathogens such as Escherichia coli, Klebsiella pneumonia, and Aeromonas caviae were present. Overall, the results of this study would hopefully enhance our knowledge about the abundance profiles of ARGs and their fate in different wastewater treatment compartments that have never been examined before.

Removal of bacterial cells, antibiotic resistance genes and integrase genes by on-site hospital wastewater treatment plants: surveillance of treated hospital effluent quality

2017 ◽  
Vol 3 (2) ◽  
pp. 293-303 ◽  
Author(s):  
Kenda Timraz ◽  
Yanghui Xiong ◽  
Hamed Al Qarni ◽  
Pei-Ying Hong
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Bacterial Cells ◽  
Hospital Wastewater ◽  
Effluent Quality ◽  
Microbial Contaminants

This study aims to evaluate the removal efficiency of microbial contaminants by wastewater treatment plants (WWTPs) operated on-site of two hospitals.

scholarly journals Swine liquid manure: a hotspot of mobile genetic elements and antibiotic resistance genes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fengxia Yang ◽  
Bingjun Han ◽  
Yanru Gu ◽  
Keqiang Zhang
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Horizontal Transfer ◽  
Antibiotic Resistance Genes ◽  
Mobile Genetic Elements ◽  
Full Scale ◽  
Liquid Manure ◽  
Piggery Wastewater ◽  
Genetic Elements ◽  
Swine Farms

Abstract The overuse or abuse of antibiotics as veterinary medicine and growth promoters accelerates antibiotic resistance, creating a serious threat to public health in the world. Swine liquid manure as an important reservoir of antibiotic resistance genes (ARGs) has received much attention, but little information is known regarding the occurrence, persistence and fate of ARGs-associated mobile genetic elements (MGEs) in swine farms, especially their change patterns and removal in full-scale piggery wastewater treatment systems (PWWTSs). In this study, we searched the presence and distribution of MGEs and associated ARGs in swine farms, and addressed their fate and seasonal variation in full-scale PWWTSs by real-time quantitative PCR (qPCR). Our results revealed class 1 integrons, class 2 integrons and conjugative plasmids were prevalent in pig feces and piggery wastewater. A clear pattern of these MGE levels in swine liquid manure was also observed, i.e., intI1 > intI2 > traA (p < 0.01), and their absolute abundances in winter were all higher than that in summer with 0.07–2.23 logs. Notably, MGEs and ARGs prevailed through various treatment units of PWWTSs, and considerable levels of them were present in the treated effluent discharged from swine farms (up to 101–107 copies/mL for MGEs and 103–108 copies/mL for ARGs). There were significant correlations between most ARG abundance and MGE levels (p < 0.05), such as tetQ and traA (r = 0.775), sul1 and intI1 (r = 0.847), qnrS and inI2 (r = 0.859), suggesting the potential of ARGs—horizontal transfer. Thus the high prevalence and enrichment of MGEs and ARGs occurred in pig feces and piggery wastewater, also implicating that swine liquid manure could be a hotspot for horizontal transfer of ARGs.

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