scholarly journals Depth resolved label-free multimodal optical imaging platform to study morpho-molecular composition of tissue

2019 ◽  
Vol 18 (5) ◽  
pp. 997-1008 ◽  
Author(s):  
Marco Andreana ◽  
Ryan Sentosa ◽  
Mikael T. Erkkilä ◽  
Wolfgang Drexler ◽  
Angelika Unterhuber
Keyword(s):  
Optical Coherence Tomography ◽  
Raman Scattering ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Structural Information ◽  
Second Harmonic ◽  
Label Free ◽  
Two Photon ◽  
Non Invasive ◽  
Anti Stokes

The presented multi-modal platform combines optical coherence tomography, two-photon excited fluorescence, second harmonic generation and anti-Stokes Raman scattering to provide molecular and structural information of tissue in a fast and non-invasive manner.

scholarly journals Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy

2016 ◽  
Vol 09 (01) ◽  
pp. 1640003 ◽  
Author(s):  
Chunqiang Li ◽  
Riikka K. Pastila ◽  
Charles P. Lin
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Immune Cells ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Label Free ◽  
Optical Signals ◽  
Two Photon

Atherosclerosis has been recognized as a chronic inflammation disease, in which many types of cells participate in this process, including lymphocytes, macrophages, dendritic cells (DCs), mast cells, vascular smooth muscle cells (SMCs). Developments in imaging technology provide the capability to observe cellular and tissue components and their interactions. The knowledge of the functions of immune cells and their interactions with other cell and tissue components will facilitate our discovery of biomarkers in atherosclerosis and prediction of the risk factor of rupture-prone plaques. Nonlinear optical microscopy based on two-photon excited autofluorescence and second harmonic generation (SHG) were developed to image mast cells, SMCs and collagen in plaque ex vivo using endogenous optical signals. Mast cells were imaged with two-photon tryptophan autofluorescence, SMCs were imaged with two-photon NADH autofluorescence, and collagen were imaged with SHG. This development paves the way for further study of mast cell degranulation, and the effects of mast cell derived mediators such as induced synthesis and activation of matrix metalloproteinases (MMPs) which participate in the degradation of collagen.

Tuning Gold Nanoparticle Self-Assembly for Optimum Coherent Anti-Stokes Raman Scattering and Second Harmonic Generation Response

2009 ◽  
Vol 113 (9) ◽  
pp. 3586-3592 ◽  
Author(s):  
Christopher J. Addison ◽  
Stanislav O. Konorov ◽  
Alexandre G. Brolo ◽  
Michael W. Blades ◽  
Robin F.B. Turner
Keyword(s):  
Raman Scattering ◽  
Second Harmonic Generation ◽  
Gold Nanoparticle ◽  
Harmonic Generation ◽  
Self Assembly ◽  
Second Harmonic ◽  
Anti Stokes

scholarly journals Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy

2011 ◽  
Vol 2 (5) ◽  
pp. 1366 ◽  
Author(s):  
Christian P. Pfeffer ◽  
Bjorn R. Olsen ◽  
Feruz Ganikhanov ◽  
François Légaré
Keyword(s):  
Skeletal Muscle ◽  
Raman Scattering ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Anti Stokes
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