Label-free imaging of brain and brain tumor specimens with combined two-photon excited fluorescence and second harmonic generation microscopy

2017 ◽  
Vol 14 (10) ◽  
pp. 105401 ◽  
Author(s):  
Liwei Jiang ◽  
Xingfu Wang ◽  
Zanyi Wu ◽  
Huiping Du ◽  
Shu Wang ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Label Free ◽  
Second Harmonic Generation Microscopy ◽  
Two Photon

scholarly journals Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy

2021 ◽  
Author(s):  
Kamdin Mirsanaye ◽  
Leonardo Uribe Castaño ◽  
Yasmeen Kamaliddin ◽  
Ahmad Golaraei ◽  
Renaldas Augulis ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Tumor Tissue ◽  
Second Harmonic ◽  
Tissue Microarrays ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Second Harmonic Generation Microscopy ◽  
Trained Classifier ◽  
Omission Rate

The extracellular matrix (ECM) collagen undergoes major remodeling during tumorigenesis. However, alterations to the ECM are not widely considered in cancer diagnostics, due to mostly uniform appearance of collagen fibers in white light images of hematoxylin and eosin-stained tissue sections. Polarimetric second-harmonic generation (P-SHG) microscopy enables label-free visualization and ultrastructural investigation of non-centrosymmetric molecules, which, when combined with texture analysis, provides multiparameter characterization of tissue collagen. This paper demonstrates whole slide imaging of breast tissue microarrays using high-throughput widefield P-SHG microscopy. The resulting P-SHG parameters are used in classification to differentiate tumor tissue from normal with 94.2% accuracy and F1-score, and 6.3% false discovery rate. Subsequently, the trained classifier is employed to predict tumor tissue with 91.3% accuracy, 90.7% F1-score, and 13.8% false omission rate. As such, we show that widefield P-SHG microscopy reveals collagen ultrastructure over large tissue regions and can be utilized as a sensitive biomarker for cancer diagnostics and prognostics studies.

scholarly journals Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy

2016 ◽  
Vol 09 (01) ◽  
pp. 1640003 ◽  
Author(s):  
Chunqiang Li ◽  
Riikka K. Pastila ◽  
Charles P. Lin
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Immune Cells ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Label Free ◽  
Optical Signals ◽  
Two Photon

Atherosclerosis has been recognized as a chronic inflammation disease, in which many types of cells participate in this process, including lymphocytes, macrophages, dendritic cells (DCs), mast cells, vascular smooth muscle cells (SMCs). Developments in imaging technology provide the capability to observe cellular and tissue components and their interactions. The knowledge of the functions of immune cells and their interactions with other cell and tissue components will facilitate our discovery of biomarkers in atherosclerosis and prediction of the risk factor of rupture-prone plaques. Nonlinear optical microscopy based on two-photon excited autofluorescence and second harmonic generation (SHG) were developed to image mast cells, SMCs and collagen in plaque ex vivo using endogenous optical signals. Mast cells were imaged with two-photon tryptophan autofluorescence, SMCs were imaged with two-photon NADH autofluorescence, and collagen were imaged with SHG. This development paves the way for further study of mast cell degranulation, and the effects of mast cell derived mediators such as induced synthesis and activation of matrix metalloproteinases (MMPs) which participate in the degradation of collagen.

scholarly journals Depth resolved label-free multimodal optical imaging platform to study morpho-molecular composition of tissue

2019 ◽  
Vol 18 (5) ◽  
pp. 997-1008 ◽  
Author(s):  
Marco Andreana ◽  
Ryan Sentosa ◽  
Mikael T. Erkkilä ◽  
Wolfgang Drexler ◽  
Angelika Unterhuber
Keyword(s):  
Optical Coherence Tomography ◽  
Raman Scattering ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Structural Information ◽  
Second Harmonic ◽  
Label Free ◽  
Two Photon ◽  
Non Invasive ◽  
Anti Stokes

The presented multi-modal platform combines optical coherence tomography, two-photon excited fluorescence, second harmonic generation and anti-Stokes Raman scattering to provide molecular and structural information of tissue in a fast and non-invasive manner.

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