Enhancement of human adipose-derived stem cell spheroid differentiation in an in situ enzyme-crosslinked gelatin hydrogel

2019 ◽  
Vol 7 (7) ◽  
pp. 1064-1075 ◽  
Author(s):  
Ching-Cheng Tsai ◽  
Yu-Jun Hong ◽  
Randall J. Lee ◽  
Nai-Chen Cheng ◽  
Jiashing Yu
Keyword(s):  
Stem Cell ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Gelatin Hydrogel ◽  
Adipose Derived Stem Cell

Standardized human adipose-derived stem cell spheroids can be harvested abundantly and the differentiation capability of cell spheroids performed well in the enzyme-crosslinked gelatin hydrogel.

scholarly journals Enzyme-Crosslinked Gelatin Hydrogel with Adipose-Derived Stem Cell Spheroid Facilitating Wound Repair in the Murine Burn Model

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2997
Author(s):  
Ting-Yu Lu ◽  
Kai-Fu Yu ◽  
Shuo-Hsiu Kuo ◽  
Nai-Chen Cheng ◽  
Er-Yuan Chuang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Three Dimensional ◽  
Stromal Vascular Fraction ◽  
Skin Wound ◽  
3D Scaffold ◽  
Skin Wound Healing ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

Engineered skin that can facilitate tissue repair has been a great advance in the field of wound healing. A well-designed dressing material together with active biological cues such as cells or growth factors can overcome the limitation of using auto-grafts from patients. Recently, many studies showed that human adipose-derived stem cells (hASCs) can be used to promote wound healing and skin tissue engineering. hASCs have already been widely applied for clinical trials. hASCs can be harvested abundantly because they can be easily isolated from fat tissue known as the stromal vascular fraction (SVF). On the other hand, increasing studies have proven that cells from spheroids can better simulate the biological microenvironment and can enhance the expression of stemness markers. However, a three-dimensional (3D) scaffold that can harbor implanted cells and can serve as a skin-repaired substitute still suffers from deficiency. In this study, we applied a gelatin/microbial transglutaminase (mTG) hydrogel to encapsulate hASC spheroids to evaluate the performance of 3D cells on skin wound healing. The results showed that the hydrogel is not toxic to the wound and that cell spheroids have significantly improved wound healing compared to cell suspension encapsulated in the hydrogel. Additionally, a hydrogel with cell spheroids was much more effective than other groups in angiogenesis since the cell spheroid has the possibility of cell–cell signaling to promote vascular generation.

In-situ gelling xyloglucan formulations as 3D artificial niche for adipose stem cell spheroids

2020 ◽  
Vol 165 ◽  
pp. 2886-2899
Author(s):  
F. Toia ◽  
A.B. Di Stefano ◽  
E. Muscolino ◽  
M.A. Sabatino ◽  
D. Giacomazza ◽  
...  
Keyword(s):  
Stem Cell ◽  
Adipose Stem Cell ◽  
Cell Spheroids ◽  
In Situ Gelling

Mesenchymal Stem Cell Spheroids Embedded in an Injectable Thermosensitive Hydrogel: An In Situ Drug Formation Platform for Accelerated Wound Healing

2020 ◽  
Vol 6 (9) ◽  
pp. 5096-5109
Author(s):  
Mohammad Ali Nilforoushzadeh ◽  
Mohsen Khodadadi Yazdi ◽  
Shaghayegh Baradaran Ghavami ◽  
Samila Farokhimanesh ◽  
Leila Mohammadi Amirabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Thermosensitive Hydrogel ◽  
Cell Spheroids

scholarly journals Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model

Nano Research ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 467-476 ◽  
Author(s):  
Aurore Van de Walle ◽  
Alexandre Fromain ◽  
Anouchka Plan Sangnier ◽  
Alberto Curcio ◽  
Luc Lenglet ◽  
...  
Keyword(s):  
Stem Cell ◽  
Iron Oxide ◽  
Real Time ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Measurement ◽  
Oxide Nanoparticles ◽  
Tissue Model ◽  
Cell Spheroid
Sign in / Sign up

Export Citation Format

Share Document