Effect of the Application Time of Accentuated Cut Edges (ACE) on Marquette Wine Phenolic Compounds

Cold-hardy interspecific hybrid grape varieties (Vitis spp.) have distinctive chemical compositions such as high acidity, a high content of anthocyanin diglucoside and a low condensed tannins content, compared to Vitis vinifera varieties. Considering the importance of phenolic compounds on the quality of red wine, a mechanical maceration technique, accentuated cut edges (ACE), has been evaluated when applied directly to crushed grapes (ACE-C), and 24 h before pressing (ACE-P), to improve the extraction of phenolic compounds. Samples were collected at crushing, bottling, and after five months of aging. Phenolic compounds and color characteristics of the wines were analyzed by high-performance liquid chromatography (HPLC) with diode array and fluorescence detectors and UV-Visible spectrophotometry. The color intensity, non-anthocyanin monomeric compounds and total iron-reactive phenolics content increased after applying ACE, compared to the control (CTL) after aging, and was significantly higher (37%) after ACE-C, compared to ACE-P. However, the concentration of condensed tannins was below the limit of detection in all the samples, indicating that ACE did not help their extraction or further interactions occurred with disrupted cell wall material. Applying ACE at crushing was considered as the optimum time to achieve a higher color stability in Marquette red wines.

Extraction and Quantification of Chlorophylls, Carotenoids, Phenolic Compounds, and Vitamins from Halophyte Biomasses

Halophytes are salt-tolerant plants, and they have been utilised as healthy, nutritious vegetables and medicinal herbs. Various studies have shown halophytes to be rich in health-beneficial compounds with antioxidant activity, anti-inflammatory and antimicrobial effects, and cytotoxic properties. Despite their potential, these plants are still underutilised in agriculture and industrial applications. This review includes the state-of-the-art literature concerning the contents of proanthocyanidins (also known as condensed tannins), total phenolic compounds, photosynthetic pigments (chlorophyll and carotenoids), and vitamins in various halophyte biomasses. Various extraction and analytical methods are also considered. The study shows that various species have exhibited potential for use not only as novel food products but also in the production of nutraceuticals and as ingredients for cosmetics and pharmaceuticals.

Condensed Tannins in White Clover (Trifolium repens) Foliar Tissues Expressing the Transcription Factor TaMYB14-1 Bind to Forage Protein and Reduce Ammonia and Methane Emissions in vitro

Grazing ruminants contribute to global climate change through enteric methane and nitrous oxide emissions. However, animal consumption of the plant polyphenolics, proanthocyanidins, or condensed tannins (CTs) can decrease both methane emissions and urine nitrogen levels, leading to reduced nitrous oxide emissions, and concomitantly increase animal health and production. CTs are largely absent in the foliage of important temperate pasture legumes, such as white clover (Trifolium repens), but found in flowers and seed coats. Attempts at enhancing levels of CT expression in white clover leaves by mutagenesis and breeding have not been successful. However, the transformation of white clover with the TaMYB14-1 transcription factor from Trifolium arvense has resulted in the production of CTs in leaves up to 1.2% of dry matter (DM). In this study, two generations of breeding elevated foliar CTs to >2% of DM. The CTs consisted predominantly of prodelphinidins (PD, 75–93%) and procyanidins (PC, 17–25%) and had a mean degree of polymerization (mDP) of approximately 10 flavan-3-ol subunits. In vitro studies showed that foliar CTs were bound to bovine serum albumin and white clover proteins at pH 6.5 and were released at pH 2.-2.5. Using rumen in vitro assays, white clover leaves containing soluble CTs of 1.6–2.4% of DM significantly reduced methane production by 19% (p ≤0.01) and ammonia production by 60% (p ≤ 0.01) relative to non-transformed wild type (WT) controls after 6 h of incubation. These results provide valuable information for further studies using CT expressing white clover leaves for bloat prevention and reduced greenhouse gas emissions in vivo.

Lead and cadmium tolerance and accumulation of proanthocyanidin- deficient mutants of the fern Athyrium yokoscense

The fern Athyrium yokoscense often flourishes around mine sites in Japan and can tolerate and accumulate heavy metals such as lead (Pb) and cadmium (Cd). In this work, we examined whether proanthocyanidins, also called condensed tannins, were involved in the mechanisms of Pb and Cd tolerance and accumulation of A. yokoscense because proanthocyanidins are known to alleviate metal stress in several plant species and are present at high levels in A. yokoscense. For this purpose, we used mutant gametophytes deficient in proanthocyanidins, in which the relative proanthocyanidin contents were 20% of those of the wild-type gametophytes. Although the proanthocyanidin contents of the mutant were quite low, the growth of the mutant was very similar to that of the wild-type gametophytes even in the presence of 80 mg/kg Pb or 48 mg/kg Cd. Under the same conditions, the mutant gametophytes also accumulated Pb and Cd as much as the wild-type gametophytes did. These results indicate that the proanthocyanidins in A. yokoscense are not important for the Pb and Cd tolerance and accumulation properties.

Use of Tannin-Containing Plants as Antimicrobials Influencing the Animal Health

The antimicrobial effects of diverse tannin-containing plants, particularly condensed tannins (CTs) produced from various plants, are the subject of this study. CT components can be determined using CT-specific procedures such the HCl-Butanol Acetone assay, Thiolysis reaction, and HPLC/MS analysis. These methods indicate CT contents, including mean degree of polymerization, the procyanidins and prodelphinidins ratio (PC/PD%), the isomers of trans- and cis-, and CT concentration. Tannin-containing plants possess antibacterial action, which can be attributed to their protein linkage technique, and tannin-type variations, particularly CTs extract and their PC/PD%. The effects of CT components on the development of Gram-positive and Gram-negative bacteria have been documented for their relative PC/PD%; this is regarded to be a key predictor of tannin characteristics in terms of antimicrobials. In conclusion, tannins, more specific CT compositions, have significant impacts on in vivo trials of animal productions and utilization of metabolites and fermentation in vitro experiments. These findings need further investigations to fully understand how CT-types act on animal feeding in terms of enhanced nutritional quality of animal diets, which may have implications for human and animal health.

Ruminal profile of completed feed as influenced by myristic and tannins addition

The study aimed to determine the effect of adding tannin and myristic acid in complete feed on nutrient content and the Profile of volatile fatty acids (VFA) in vitro. The materials used for this research were corn straw, condensed tannins, myristic acid, coffee waste, rice bran, tapioca byproduct, soybean meal, copra meal, and palm kernel meal. The method was used in this experiment was laboratory experiment with four treatments and three replications. The treatment consisted of T1 complete feed (40% corn straw + 60% concentrate (be based DM)), T2 (complete feed + condensed tannins 3%/kg DM and myristic acid 2%/kg DM), T3 (complete feed + condensed tannins 3%/kg DM and myristic acid 3%/kg DM), and T4 (complete feed + condensed tannins 3%/kg DM and myristic acid 4%/kg DM). Every treatment was repeated three times and analysed statistically using Randomized Block Design (RBD). The adding of 4% myristic acid to the complete feed (T4) gives the best results, it increased nutrition and propionic acid, but decreased acetic acid, butyric acid, total VFA and C2/C3 ratio.