A quadruple-label time-resolved fluorescence immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

2020 ◽  
Vol 12 (3) ◽  
pp. 247-254 ◽  
Author(s):  
Xiaoxi Chang ◽  
Yaqing Zhang ◽  
Hebing Liu ◽  
Xiaoqi Tao
Keyword(s):  
Quantitative Determination ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Label Time

A quadruple-label time-resolved fluorescence immunochromatographic assay (QL-TRFICA) for simultaneous quantitative detection of three mycotoxins (AFB1, ZEN and OTA) in 6 grains (corn, soybean, sorghum, wheat, rice and oats) was established.

Europium nanosphere-based fluorescence strip sensor for ultrasensitive and quantitative determination of fumonisin B1

2020 ◽  
Vol 12 (43) ◽  
pp. 5229-5235
Author(s):  
Lingling Guo ◽  
Zhongxing Wang ◽  
Xinxin Xu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Rapid Detection ◽  
Detection Method ◽  
Fumonisin B1 ◽  
Immunochromatographic Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

The developed time-resolved fluorescence immunochromatographic assay can be widely applicable as an ultrasensitive, rapid detection method for FB1 in grains.

A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator

2019 ◽  
Vol 584 ◽  
pp. 113384 ◽  
Author(s):  
Michał Szkop ◽  
Kamil Brygoła ◽  
Magdalena Janczewska ◽  
Tomasz Ciach
Keyword(s):  
Quantitative Determination ◽  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

scholarly journals A Dual-Label Time-Resolved Fluorescence Immunoassay for the Simultaneous Determination of Cardiac Troponin T and Myoglobin

2016 ◽  
Vol 22 (2) ◽  
pp. 130-135 ◽  
Author(s):  
Ling Wang ◽  
Mujuan Xu ◽  
Ruolan Huang ◽  
Xiao Chang ◽  
Cuicui Chen ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Troponin T ◽  
Correlation Coefficients ◽  
Linear Dynamic Range ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Dual Label ◽  
Label Time

The aim of this study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of cardiac troponin T (cTnT) and myoglobin (MYO) for the early diagnosis of acute myocardial infarction. The sandwich immunoassay was used to detect the concentration of cTnT and MYO in serum. cTnT and MYO in serum were captured by anti-cTnT and anti-MYO antibodies immobilized on microtiter wells and then banded together with another anti-cTnT and anti-MYO labeled with europium(III) Sm3+ and samarium(III) Eu3+ chelates, followed by fluorescence measurement using time-resolved fluorometry. The performance of this TRFIA was evaluated using the clinical serum and compared with the commercial assays. The linear correlation coefficients ( R2) of the cTnT and MYO standard curves were 0.9993 and 0.9995, respectively. The sensitivity for cTnT detection was 2.21 pg/mL (linear dynamic range was 3.24–963.71 pg/mL), and the average recovery was 100.57%. The sensitivity for MYO detection was 3.24 ng/mL (linear dynamic range was 4.95–976.85 ng/mL), and the average recovery was 99.79%. High correlation coefficients ( R2) were obtained between the commercial assays and dual-label TRFIA ( R2 = 0.999). The present dual-label TRFIA has high sensitivity, specificity, and accuracy in clinical sample analysis. It is a good alternative to the single-label diagnostic methods.

Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

2017 ◽  
Vol 9 (45) ◽  
pp. 6430-6434 ◽  
Author(s):  
Yi Zhang ◽  
Xin-Liang Ding ◽  
Ming-Ming Guo ◽  
Ting-Ting Han ◽  
Zi-Jian Huang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved

In the present study, a novel time-resolved fluorescence immunochromatographic assay was established for the rapid quantitative detection of microcystin-leucine arginine (MC-LR).

scholarly journals Portable, Rapid, and Sensitive Time-Resolved Fluorescence Immunochromatography for On-Site Detection of Dexamethasone in Milk and Pork

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1339
Author(s):  
Xiangmei Li ◽  
Xiaomin Chen ◽  
Jinxiao Wu ◽  
Zhiwei Liu ◽  
Jin Wang ◽  
...  
Keyword(s):  
Technical Support ◽  
Rapid Screening ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Screening Methods ◽  
Time Resolved Fluorescence ◽  
Fluorescent Microspheres ◽  
Time Resolved ◽  
Coefficients Of Variation ◽  
Pork Samples

Dexamethasone (DEX) is widely used because of its anti-inflammatory, anti-endotoxin, anti-shock, and stress-enhancing response activities. It can increase the risk of diabetes and hypertension if it is abused or used improperly. However, there is a lack of sensitive and rapid screening methods for DEX in food. In this study, a time-resolved fluorescent microspheres immunochromatographic assay (TRFM-ICA) integrated with a portable fluorescence reader was developed for the quantitative detection of DEX in milk and pork. The cut-off values of the TRFM-ICA were 0.25 ng/mL and 0.7 µg/kg, respectively. The limits of quantitation (LOQs) were 0.003 ng/mL and 0.062 µg/kg, respectively. The recovery rates were 80.0–106.7%, and 78.6–83.6%, respectively, with the coefficients of variation ranging 6.3–12.5%, and 7.5–10.3%, respectively. A parallel experiment for 20 milk and 10 pork samples with LC-MS/MS was carried out to confirm the performance of the on-site application of the developed TRFM-ICA. The results of the two methods are basically the same; the correlation (R2) was >0.98. The establishment of TRFM-ICA will provide a new sensitive and efficient technical support for the rapid screening of DEX in food.

Sign in / Sign up

Export Citation Format

Share Document