Development of Recyclable Magnetic Cross Linked Enzyme Aggregates for the Synthesis of High Value Rare Sugar D-Tagatose in Aqueous Phase Catalysis

Catalysis Science & Technology ◽

10.1039/d0cy02397c ◽

2021 ◽

Author(s):

Shushil Rai ◽

Varun Kumar ◽

Sudesh Yadav

Keyword(s):

Aqueous Phase ◽

Rare Sugar ◽

Arabinose Isomerase ◽

Aqueous Phase Catalysis

In this study, a high value rare sugar D-Tagatose was synthesized using recyclable magnetic catalysts. The magnetic cross linked enzyme aggregate (mCLEA) of L-Arabinose isomerase (L-AI) was developed for the...

Download Full-text

Hydroformylation by supported aqueous-phase catalysis: a new class of heterogeneous catalysts

Nature ◽

10.1038/339454a0 ◽

1989 ◽

Vol 339 (6224) ◽

pp. 454-455 ◽

Cited By ~ 251

Author(s):

Juan P. Arhancet ◽

Mark E. Davis ◽

Joseph S. Merola ◽

Brian E. Hanson

Keyword(s):

Aqueous Phase ◽

Heterogeneous Catalysts ◽

New Class ◽

Supported Aqueous Phase ◽

Aqueous Phase Catalysis

Download Full-text

New water soluble chelating phosphines for aqueous phase catalysis

Journal of Molecular Catalysis A Chemical ◽

10.1016/s1381-1169(97)00063-0 ◽

1997 ◽

Vol 124 (1) ◽

pp. 21-28 ◽

Cited By ~ 36

Author(s):

Hao Ding ◽

Jianxing Kang ◽

Brian E. Hanson ◽

Christian W. Kohlpaintner

Keyword(s):

Aqueous Phase ◽

Water Soluble ◽

Chelating Phosphines ◽

Aqueous Phase Catalysis

Download Full-text

Hydrocarboxylation of olefins by supported aqueous-phase catalysis

Catalysis Today ◽

10.1016/j.cattod.2014.08.033 ◽

2015 ◽

Vol 247 ◽

pp. 147-154 ◽

Cited By ~ 7

Author(s):

Zhenhong He ◽

Zhenshan Hou ◽

Yagang Zhang ◽

Tianfu Wang ◽

Yierxiati Dilixiati ◽

...

Keyword(s):

Aqueous Phase ◽

Supported Aqueous Phase ◽

Aqueous Phase Catalysis

Download Full-text

Models for Supported Aqueous-Phase Catalysis

Aqueous Organometallic Chemistry and Catalysis ◽

10.1007/978-94-011-0355-8_19 ◽

1995 ◽

pp. 195-197

Author(s):

I. Tóth ◽

I. Guo ◽

B. E. Hanson

Keyword(s):

Aqueous Phase ◽

Supported Aqueous Phase ◽

Aqueous Phase Catalysis

Download Full-text

A Three-Step Process for the Bioconversion of Whey Permeate into a Glucose-Free D-Tagatose Syrup

Catalysts ◽

10.3390/catal10060647 ◽

2020 ◽

Vol 10 (6) ◽

pp. 647 ◽

Cited By ~ 1

Author(s):

Fadia V. Cervantes ◽

Sawssan Neifar ◽

Zoran Merdzo ◽

Javier Viña-Gonzalez ◽

Lucia Fernandez-Arrojo ◽

...

Keyword(s):

Bacillus Stearothermophilus ◽

Cheese Whey ◽

Bifidobacterium Bifidum ◽

Rare Sugar ◽

Experimental Conditions ◽

Whey Permeate ◽

Mild Conditions ◽

Stage Process ◽

Short Time ◽

Arabinose Isomerase

We have developed a sustainable three-stage process for the revaluation of cheese whey permeate into D-tagatose, a rare sugar with functional properties used as sweetener. The experimental conditions (pH, temperature, cofactors, etc.) for each step were independently optimized. In the first step, concentrated whey containing 180–200 g/L of lactose was fully hydrolyzed by β-galactosidase from Bifidobacterium bifidum (Saphera®) in 3 h at 45 °C. Secondly, glucose was selectively removed by treatment with Pichia pastoris cells for 3 h at 30 °C. The best results were obtained with 350 mg of cells (previously grown for 16 h) per mL of solution. Finally, L-arabinose isomerase US100 from Bacillus stearothermophilus was employed to isomerize D-galactose into D-tagatose at pH 7.5 and 65 °C, in presence of 0.5 mM MnSO4. After 7 h, the concentration of D-tagatose was approximately 30 g/L (33.3% yield, referred to the initial D-galactose present in whey). The proposed integrated process takes place under mild conditions (neutral pH, moderate temperatures) in a short time (13 h), yielding a glucose-free syrup containing D-tagatose and galactose in a ratio 1:2 (w/w).

Download Full-text