scholarly journals A Three-Step Process for the Bioconversion of Whey Permeate into a Glucose-Free D-Tagatose Syrup

Catalysts ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 647 ◽  
Author(s):  
Fadia V. Cervantes ◽  
Sawssan Neifar ◽  
Zoran Merdzo ◽  
Javier Viña-Gonzalez ◽  
Lucia Fernandez-Arrojo ◽  
...  
Keyword(s):  
Bacillus Stearothermophilus ◽  
Cheese Whey ◽  
Bifidobacterium Bifidum ◽  
Rare Sugar ◽  
Experimental Conditions ◽  
Whey Permeate ◽  
Mild Conditions ◽  
Stage Process ◽  
Short Time ◽  
Arabinose Isomerase

We have developed a sustainable three-stage process for the revaluation of cheese whey permeate into D-tagatose, a rare sugar with functional properties used as sweetener. The experimental conditions (pH, temperature, cofactors, etc.) for each step were independently optimized. In the first step, concentrated whey containing 180–200 g/L of lactose was fully hydrolyzed by β-galactosidase from Bifidobacterium bifidum (Saphera®) in 3 h at 45 °C. Secondly, glucose was selectively removed by treatment with Pichia pastoris cells for 3 h at 30 °C. The best results were obtained with 350 mg of cells (previously grown for 16 h) per mL of solution. Finally, L-arabinose isomerase US100 from Bacillus stearothermophilus was employed to isomerize D-galactose into D-tagatose at pH 7.5 and 65 °C, in presence of 0.5 mM MnSO4. After 7 h, the concentration of D-tagatose was approximately 30 g/L (33.3% yield, referred to the initial D-galactose present in whey). The proposed integrated process takes place under mild conditions (neutral pH, moderate temperatures) in a short time (13 h), yielding a glucose-free syrup containing D-tagatose and galactose in a ratio 1:2 (w/w).

scholarly journals Kinetics of Non-Enzymatic Synthesis of Dipeptide Cbz-Phe-Leu with AOT Reversed Micelles

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1003
Author(s):  
Michiaki Matsumoto ◽  
Tadashi Hano
Keyword(s):  
Enzymatic Synthesis ◽  
Maximum Yield ◽  
Side Reactions ◽  
Experimental Conditions ◽  
Operational Conditions ◽  
Ph Dependency ◽  
Optimum Water Content ◽  
Short Time ◽  
Kinetics Of ◽  
Aot Reversed Micelles

The non-enzymatic synthesis of N-benzyloxycarbonyl-L-phenylalanyl-L-leucine (Cbz-Phe-Leu) from lipophilic N-benzyloxycarbonyl-L-phenylalanine (Cbz-Phe) and hydrophilic L-leucine (Leu), by N, N’-dicyclohexylcarbodiimide (DCC) as a condensing agent, was carried out using a reversed micellar system composed of bis(2-ethylhexyl) sodium sulfosuccinate (AOT) as a surfactant and isooctane. We successfully synthesized Cbz-Phe-Leu in a short time and investigated the effects of its operational conditions, the DCC concentration, w0, and the pH on the kinetic parameters and the maximum yields. For dipeptide synthesis, we had to add an excess of DCC with the substrates because of the side reactions of Cbz-Phe. From the pH dependency of the reactivity, a partially cationic form of Leu was better for a synthesis reaction because of the enrichment of Leu at the interface by anionic AOT. The optimum water content on the dipeptide synthesis was w0 = 28 due to the competition of the peptide synthesis and the side reactions. The maximum yield of Cbz-Phe-Leu was 0.565 at 80 h under optimum experimental conditions.

scholarly journals Production of lactulose oligosaccharides by isomerisation of transgalactosylated cheese whey permeate obtained by β-galactosidases from dairy Kluyveromyces

2015 ◽  
Vol 82 (3) ◽  
pp. 356-364 ◽  
Author(s):  
Beatriz Padilla ◽  
Florencia Frau ◽  
Ana Isabel Ruiz-Matute ◽  
Antonia Montilla ◽  
Carmela Belloch ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Food Industry ◽  
Cheese Whey ◽  
Kluyveromyces Lactis ◽  
Sodium Aluminate ◽  
Whey Permeate ◽  
Total Carbohydrates ◽  
Cheese Whey Permeate

β-Galactosidases from Kluyveromyces lactis and Kluyveromyces marxianus isolated from artisanal ewes’ milk cheeses, were used to transgalactosylate lactose from cheese whey permeate (WP). The content of galactooligosaccharides (GOS) obtained by transgalactosylation was comparable with that formed using pure lactose as substrate. In order to obtain a mixture with higher prebiotic oligosaccharide content, isomerisation of the transgalactosylated WP was carried out using sodium aluminate as catalyst. The transgalactosylated mixtures at 6 h of reaction contained amounts of prebiotic carbohydrates (tagatose, lactulose, GOS and oligosaccharides derived from lactulose, OsLu) close to 50 g/100 g of total carbohydrates for all the strains tested, corresponding to 322 g prebiotics/kg whey permeate. Thus, the suitability of this methodology to produce mixtures of dietary non-digestible carbohydrates with prebiotic properties from WP has been demonstrated, which is interesting for the food industry since it increases the value and the applicability of this by-product from cheese manufacture.

Growing commodity tilapia in various experimental conditions

2020 ◽  
pp. 26-36
Author(s):  
F. A. Altayeva
Keyword(s):  
Fish Farming ◽  
Biological Indicators ◽  
Fish Farms ◽  
Experimental Conditions ◽  
Fish Breeding ◽  
Closed Water ◽  
The Republic ◽  
Short Time ◽  
High Fish ◽  
Experimental Ponds

The article presents the results of growing tilapia to marketable weight in experimental sites (Chilikskoye pond pond farms LLP, KATU named after S. Seifullin JSC and Kapshagai spawning farm — 1973 LLP). In the process of carrying out research works, the methods generally accepted in fish farming, hydrochemistry, hydrobiology, hydrology, ichthyology, as well as foreign normative and technical guidelines and methodological instructions on the technology of growing commercial tilapia in industrial conditions were used. Monitoring of hydrochemical analyses of water in ponds, swimming pools, and closed water supply installations is given. Fish-breeding and biological indicators of tilapia grown in various experimental conditions are presented. The results of the studies have shown the real possibility of growing tilapia in various conditions of fish farms in Kazakhstan. Based on the results of the work, the following conclusions were made: — satisfactory fish-breeding indicators were obtained with various cultivation technologies (as when growing tilapia in polyculture and monoculture in ponds, in pools, as well as cultivation in the recirculation system); — the cultivation of tilapia in the experimental ponds of the fish farm of the Almaty region of the Republic of Kazakhstan has shown the possibility of obtaining high fish-breeding biological indicators of tilapia and the ability to grow to marketable mass in a short time; — as a result of experimental cultivation in various conditions, it was found that the survival rate was not lower than 97%; — as a result of the analysis of the profitability indices of the developed technologies, it was found that the most profitable was the technology of growing marketable tilapia products in monoculture ponds on water with a natural thermal regime in Chilik Pond Economy LLP.

Metal-free phosphonation of heteroarene N-oxides with trialkyl phosphite at room temperature

2017 ◽  
Vol 15 (15) ◽  
pp. 3165-3169 ◽  
Author(s):  
Ming-Tao Chen ◽  
Xia You ◽  
Li-Gang Bai ◽  
Qun-Li Luo
Keyword(s):  
Room Temperature ◽  
Trialkyl Phosphite ◽  
Metal Free ◽  
Mild Conditions ◽  
Short Time

Heteroarene N-oxides were successfully converted to heteroarylphosphonates in a very short time under mild conditions through in situ activation with CBrCl3.

Cheese whey permeate fermentation by Kluyveromyces lactis: a combined approach to wastewater treatment and bioethanol production

2019 ◽  
Vol 41 (24) ◽  
pp. 3210-3218 ◽  
Author(s):  
Fábio Coelho Sampaio ◽  
Janaína Teles de Faria ◽  
Milena Fernandes da Silva ◽  
Ricardo Pinheiro de Souza Oliveira ◽  
Attilio Converti
Keyword(s):  
Wastewater Treatment ◽  
Cheese Whey ◽  
Kluyveromyces Lactis ◽  
Bioethanol Production ◽  
Combined Approach ◽  
Whey Permeate ◽  
Cheese Whey Permeate

Expert System for EPMA

1999 ◽  
Vol 5 (S2) ◽  
pp. 592-593
Author(s):  
C. Fournier ◽  
C. Merlet ◽  
P.F. Staub ◽  
O. Dugne
Keyword(s):  
Quantitative Analysis ◽  
Expert System ◽  
Reasonable Accuracy ◽  
Experimental Conditions ◽  
Sample Composition ◽  
Starting Point ◽  
Accuracy Of Results ◽  
Short Time ◽  
And Control ◽  
Selection Of

Analysis parameters for an electron microprobe are numerous, and the accuracy of the quantitative analysis is very sensitive to the selection of these experimental conditions. The expert system intends to optimize the choice of each analysis parameter as well as to automate the phases of a quantitative analysis on all kinds of materials. To summarise, the aim of the expert system is to simplify the procedures, improve the accuracy of results and control the analysis time. The figure 1 illustrates the various stages of the expert system.The starting point of the expert system is an interactive questionnaire concerning the sample,( ie, what is already known), and about the expectations on the analysis,( ie, the accuracy of the results and/or the duration of the analysis required by the operator). Then, the expert system performs a semi-quantitative analysis on the sample. Based on the acquisition of a wavelength qualitative spectrum, this method is a way to obtain the sample composition in a short time with the advantages of the WDS system, and with a reasonable accuracy.

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