Effect of In Vitro Gingival Fibroblast Seeding on the In Vivo Incorporation of Acellular Dermal Matrix Allografts in Dogs

The success rate of grafting using acellular dermal matrix (ADM) for chronic tympanic membrane was reported in previous studies to be lower than fascia or perichondrium. Combining mesenchymal stem cells (MSCs) and growth factor-loaded ADM for the regeneration of chronic TMP has not been reported so far. In this study, we hypothesized that combining growth factor-loaded ADM/MSCs could promote the recruitment of MSCs and assist in TMP regeneration. We evaluated the regeneration and compared the performance of four scaffolds in both in vitro and in vivo studies. MTT, qPCR, and immunoblotting were performed with MSCs. In vivo study was conducted in 4 groups (control; ADM only, ADM/MSC, ADM/MSC/bFGF, ADM/MSC/EGF) of rats and inferences were made by otoendoscopy and histological changes. Attachment of MSCs on ADM was observed by confocal microscopy. Proliferation rate increased with time in all treated cells. Regeneration-related gene expression in the treated groups was higher. Also, graft success rate was significantly higher in ADM/MSC/EGF group than other groups. Significant relationships were disclosed in neodrum thickness between each group. The results suggest, in future, combining EGF with ADM/MSCs could possibly be used as an outpatient treatment, without the need for surgery for eardrum regeneration.

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Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix

Brazilian Dental Journal ◽

10.1590/s0103-64402010000300001 ◽

2010 ◽

Vol 21 (3) ◽

pp. 179-189 ◽

Cited By ~ 12

Author(s):

Annelissa Zorzeto Rodrigues ◽

Paulo Tambasco de Oliveira ◽

Arthur Belém Novaes Jr. ◽

Luciana Prado Maia ◽

Sérgio Luís Scombatti de Souza ◽

...

Keyword(s):

Cell Monolayer ◽

Acellular Dermal Matrix ◽

Human Gingival Fibroblasts ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Gingival Fibroblasts ◽

Dermal Matrix ◽

The Matrix ◽

Acellular Dermal

The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.

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Alveolar ridge preservation with fibroblast growth factor‐2 modified acellular dermal matrix membrane and a bovine‐derived xenograft. An experimental in vivo study.

Clinical Oral Implants Research ◽

10.1111/clr.13749 ◽

2021 ◽

Author(s):

Ruolin Wang ◽

Wenhua Liu ◽

Hongmei Guo ◽

Shaohua Ge ◽

Haiyun Huang ◽

...

Keyword(s):

Growth Factor ◽

Acellular Dermal Matrix ◽

Fibroblast Growth Factor 2 ◽

Ridge Preservation ◽

Alveolar Ridge ◽

Fibroblast Growth ◽

Dermal Matrix ◽

Alveolar Ridge Preservation ◽

Acellular Dermal

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Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix

Materials Science Forum ◽

10.4028/www.scientific.net/msf.610-613.1298 ◽

2009 ◽

Vol 610-613 ◽

pp. 1298-1301

Author(s):

Xiang Rong Zhang ◽

De Wu Liu ◽

Guang Hua Guo ◽

Yan Peng

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Gradient Centrifugation ◽

Human Mesenchymal Stem Cells ◽

Acellular Dermal Matrix ◽

Culture Method ◽

Gene Encoding ◽

Dermal Matrix ◽

Acellular Dermal

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.

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