scholarly journals Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell

2021 ◽  
Author(s):  
Yongzheng Cong ◽  
Khatereh Motamedchaboki ◽  
Santosh A. Misal ◽  
Yiran Liang ◽  
Amanda J. Guise ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Single Cell ◽  
Mammalian Cell ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Low Flow ◽  
Proteome Profiling ◽  
Cell Proteome

The combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS) and latest-generation mass spectrometry instrumentation provides dramatically improved single-cell proteome profiling.

Calculation of ion mobilities from electrospray ionization high-field asymmetric waveform ion mobility spectrometry mass spectrometry

2001 ◽  
Vol 114 (23) ◽  
pp. 10270-10277 ◽  
Author(s):  
Roger Guevremont ◽  
David A. Barnett ◽  
Randy W. Purves ◽  
Larry A. Viehland
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Asymmetric Waveform
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