scholarly journals On the lipid flip-flop and phase transition coupling

Soft Matter ◽  
2020 ◽  
Vol 16 (33) ◽  
pp. 7696-7703
Author(s):  
Lionel Porcar ◽  
Yuri Gerelli
Keyword(s):  
Phase Transition ◽  
Lipid Bilayers ◽  
Neutron Reflectometry ◽  
Supported Lipid Bilayers ◽  
Lipid Phase ◽  
Lipid Phase Transition ◽  
Flip Flop

We measured by neutron reflectometry the loss of asymmetry in solid supported lipid bilayers, revealing an intrinsic interplay between passive lipid flip-flop and lipid phase transition.

Formation of supported lipid bilayers on silica: relation to lipid phase transition temperature and liposome size

Soft Matter ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Yujia Jing ◽  
Hana Trefna ◽  
Mikael Persson ◽  
Bengt Kasemo ◽  
Sofia Svedhem
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Lipid Bilayers ◽  
Phase Transition Temperature ◽  
Supported Lipid Bilayers ◽  
Lipid Phase ◽  
Lipid Phase Transition ◽  
Liposome Size

scholarly journals Verapamil prevents the effects of daunomycin on the thermotropic phase transition of model lipid bilayers

1991 ◽  
Vol 279 (2) ◽  
pp. 413-418 ◽  
Author(s):  
J M Canaves ◽  
J A Ferragut ◽  
J M Gonzalez-Ros
Keyword(s):  
Phase Transition ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Drug Binding ◽  
Lipid Phase ◽  
Dependent Manner ◽  
Binding Studies ◽  
Scanning Calorimetry ◽  
Lipid Phase Transition ◽  
Fluorescence Depolarization

High-sensitivity differential scanning calorimetry and fluorescence-depolarization techniques were used to study how the presence of daunomycin and/or verapamil affect the thermotropic behaviour of dipalmitoyl phosphatidylcholine (DPPC) vesicles. Daunomycin, a potent anti-cancer agent, perturbs the thermodynamic parameters associated with the lipid phase transition: it decreases the enthalpy change, lowers the transition temperature and reduces the co-operative behavior of the phospholipid molecules. Verapamil, on the other hand, produces smaller alterations in the lipid phase transition. However, when daunomycin and verapamil are present simultaneously in the DPPC vesicles, it is observed that verapamil prevents, in a concentration-dependent manner, the alteration in the phospholipid phase transition expected from the presence of daunomycin in the bilayer. Furthermore, drug-binding studies suggest that the observed interference of verapamil in the daunomycin/phospholipid interaction occurs without a decrease in the amount of daunomycin bound to the lipid bilayer and without the formation of a daunomycin-verapamil complex. Because of the importance of drug-membrane interactions in anthracycline cytotoxicity, we speculate that the lipid bilayer of biological membranes may provide appropriate sites at which the presence of verapamil influences the activity of daunomycin.

Fatty acyl chain structure, orientational order, and the lipid phase transition in Acholeplasma laidlawii B membranes. A review of recent 19F nuclear magnetic resonance studies

1984 ◽  
Vol 62 (11) ◽  
pp. 1134-1150 ◽  
Author(s):  
P. M. Macdonald ◽  
B. D. Sykes ◽  
R. N. McElhaney
Keyword(s):  
Phase Transition ◽  
Fatty Acids ◽  
Nuclear Magnetic Resonance ◽  
Acyl Chain ◽  
Orientational Order ◽  
Membrane Lipid ◽  
Fatty Acyl ◽  
Fatty Acyl Chain ◽  
Lipid Phase ◽  
Lipid Phase Transition

The orientational order parameters of monofluoropalmitic acids biosynthetically incorporated into membranes of Acholeplasma laidlawii B in the presence of a large excess of a variety of structurally diverse fatty acids have been determined via 19F nuclear magnetic resonance (19F NMR) spectroscopy. It is demonstrated that these monofluoropalmitic acids are relatively nonperturbing membrane probes based upon physical (differential scanning calorimetry), biochemical (membrane lipid analysis), and biological (growth studies) criteria. 19F NMR is shown to convey the same qualitative and quantitative picture of membrane lipid order provided by 2H-NMR techniques and to be sensitive to the structural characteristics of the membrane fatty acyl chains, as well as to the lipid phase transition. Representatives of each naturally occurring class of fatty acyl chain structures, including straight-chain saturated, methyl-branched, monounsaturated, and alicyclic-ring-substituted fatty acids, were studied and the 19F-NMR order parameters were correlated with the lipid phase transitions (determined calorimetrically). The lipid phase transition was the prime determinant of overall orientational order regardless of fatty acid structure. Effects upon orientational order attributable to specific structural substituents were discernible, but were secondary to the effects of the lipid phase transition. In the gel state, relative overall order was directly proportional to the temperature of the particular lipid phase transition. Not only the overall order, but also the order profile across the membrane was sensitive to the presence of particular structural substituents. In particular, in the gel state specific fatty acyl structures demonstrated a characteristic disordering effect in the membrane order profile. These various observations can be merged to provide a unified picture of the manner in which fatty acyl chain chemistry modulates the physical state of membrane lipids.

The incorporation of cholesterol into inner mitochondrial membranes and its effect on lipid phase transition

1980 ◽  
Vol 599 (2) ◽  
pp. 528-537 ◽  
Author(s):  
Thomas D. Madden ◽  
Carmen Vigo ◽  
K.Richard Bruckdorfer ◽  
Dennis Chapman
Keyword(s):  
Phase Transition ◽  
Lipid Phase ◽  
Mitochondrial Membranes ◽  
Lipid Phase Transition

The Image-Based Observation of the LβI-to-Lβ‘ Phase Transition in Solid-Supported Lipid Bilayers

Langmuir ◽  
2001 ◽  
Vol 17 (16) ◽  
pp. 5121-5124 ◽  
Author(s):  
Robert L. McClain ◽  
J. J. Breen
Keyword(s):  
Phase Transition ◽  
Lipid Bilayers ◽  
Supported Lipid Bilayers

Some Aspects of Lipid-Phase Transition on Membrane Permeability and Lipid-Protein Association

1977 ◽  
pp. 38-46 ◽  
Author(s):  
M. C. Blok ◽  
L. L. M. van Deenen ◽  
J. de Gier ◽  
J. A. F. Op den Kamp ◽  
A. J. Verkleij
Keyword(s):  
Phase Transition ◽  
Membrane Permeability ◽  
Lipid Phase ◽  
Lipid Phase Transition ◽  
Protein Association
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