Surface plasmon resonance imaging of the heterogeneous electric double-layer distribution and its change dynamics

2020 ◽  
Vol 8 (39) ◽  
pp. 13563-13573
Author(s):  
Xueyi Luo ◽  
Shijie Deng ◽  
Peng Wang

A surface plasmon resonance (SPR) imaging method for monitoring the electric double-layer (EDL) change dynamics in real-time and map the lateral EDL distribution on heterogeneous electrode/electrolyte interface.

The Analyst ◽  
2017 ◽  
Vol 142 (9) ◽  
pp. 1471-1481 ◽  
Author(s):  
Patjaree Peungthum ◽  
Krisda Sudprasert ◽  
Ratthasart Amarit ◽  
Armote Somboonkaew ◽  
Boonsong Sutapun ◽  
...  

Fewer steps for ABO subgroup identification by using the SPR imaging incorporating an antibody array was presented.


2014 ◽  
Vol 53 (26) ◽  
pp. 6037 ◽  
Author(s):  
Pengfei Zhang ◽  
Le Liu ◽  
Yonghong He ◽  
Zhiyuan Shen ◽  
Jun Guo ◽  
...  

RSC Advances ◽  
2016 ◽  
Vol 6 (26) ◽  
pp. 21900-21906 ◽  
Author(s):  
Xiao Wang ◽  
Jiying Xu ◽  
Chanjuan Liu ◽  
Yi Chen

A surface plasmon resonance imaging method to differentiate the interaction between the protein human high mobility group box 1 or human nuclear protein positive cofactor 4 (PC4) and DNAs has been developed.


RSC Advances ◽  
2018 ◽  
Vol 8 (50) ◽  
pp. 28266-28274 ◽  
Author(s):  
Xueyi Luo ◽  
Shijie Deng ◽  
Peng Wang

This paper provides a novel method for temporal–spatial-resolved mapping of the electrical double layer changes at the heterogeneous interface.


2015 ◽  
Vol 1131 ◽  
pp. 88-94
Author(s):  
Chokchai Puttharugsa ◽  
Oraprapai Gajanandana ◽  
Orawan Himananto ◽  
Ratthasart Amarit ◽  
Armote Somboonkaew ◽  
...  

Signal enhancement based surface plasmon resonance imaging (SPR imaging) was developed using gold nanoparticle (AuNP) for detection of Acidovorax avenae subsp. citrulli (Aac). The antibodies against Aac, monoclonal antibody 11E5 (MAb 11E5) and polyclonal antibody MPC (PAb MPC), were covalently immobilized on the 1:40 of mixed self-assembled monolayer (mixed SAMs) surface for detection of Aac. The 107 cfu/ml of Aac was injected over the surface and was captured by immobilized antibodies on the sensing surface. PAb MPC conjugated to 10 nm of gold nanoparticle (PAb-AuNP) was flowed over the surface to enhance the SPR signal for detection of Aac. The MAb/Aac/PAb-AuNP assay provides a higher in signal enhancement than that of PAb/Aac/PAb-AuNP assay. Moreover, SPR signal using PAb-AuNP enhancement increases 23 – fold in signal enhancement when comparing to PAb enhancement. Thus, the detection of Aac based SPR imaging can be used PAb-AuNP in signal enhancement for further improvement in limit of detection (LOD).


BioTechniques ◽  
2021 ◽  
Author(s):  
Michal Lesnak ◽  
Dominik Jursa ◽  
Marek Miskay ◽  
Helena Riedlova ◽  
Karla Barcova ◽  
...  

Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C.


2006 ◽  
Vol 4 (4) ◽  
pp. 610 ◽  
Author(s):  
Roberta D'Agata ◽  
Giulia Grasso ◽  
Giuseppe Iacono ◽  
Giuseppe Spoto ◽  
Graziella Vecchio

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