Photoelectrochemical Analysis of Alkaline Phosphatase Activity in Single Living Cells

The Analyst ◽  
2021 ◽  
Author(s):  
Nina Wang ◽  
Rongrong Pan ◽  
Li-Na Ji ◽  
Dechen Jiang ◽  
Hong-Yuan Chen
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Living Cells ◽  
Tin Oxides ◽  
Single Living Cells ◽  
Single Living

Conventional photoelectrochemical (PEC) analysis mostly utilizes the photoactive material modified planar indium tin oxides (ITOs) to obtain photocurrent responses for the measurement of analytes in the solution. In this work,...

A turn-on near-infrared fluorescent probe for visualization of endogenous alkaline phosphatase activity in living cells and zebrafish

The Analyst ◽  
2021 ◽  
Author(s):  
Xiao Pang ◽  
Yaqian Li ◽  
Qiujun Lu ◽  
Ziqi Ni ◽  
Zile Zhou ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Fluorescent Probe ◽  
Alkaline Phosphatase Activity ◽  
Near Infrared ◽  
Living Cells ◽  
Turn On ◽  
Living Organisms

Alkaline phosphatase (ALP) is an essential hydrolase and widely distributed in living organisms.

A fluorescent turn-on probe for the detection of alkaline phosphatase activity in living cells

2011 ◽  
Vol 47 (35) ◽  
pp. 9825 ◽  
Author(s):  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Yongdoo Choi ◽  
Youngmi Kim
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Living Cells ◽  
Turn On

Reaction-Based Off–On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice

2017 ◽  
Vol 9 (8) ◽  
pp. 6796-6803 ◽  
Author(s):  
Yi Tan ◽  
Ling Zhang ◽  
Ka Ho Man ◽  
Raoul Peltier ◽  
Ganchao Chen ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Fluorescent Probe ◽  
Alkaline Phosphatase Activity ◽  
Near Infrared ◽  
Living Cells

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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