Activatable molecular probes for fluorescence-guided surgery, endoscopy and tissue biopsy

2022 ◽  
Author(s):  
Yan Zhang ◽  
Guopeng Zhang ◽  
Ziling Zeng ◽  
Kanyi Pu
Keyword(s):  
Real Time ◽  
Molecular Probes ◽  
Dynamic Visualization ◽  
Optical Signals ◽  
Time Dynamic ◽  
Guided Surgery ◽  
Tissue Biopsy ◽  
Fluorescence Guided Surgery

We highlight the development of activatable molecular probes that trigger the optical signals toward biomarkers, allowing real-time, dynamic visualization of lesions and margins for guided-surgery, endoscopy and tissue biopsy with molecular precision.

Simultaneous real-time multicomponent fluorescence and reflectance imaging method for fluorescence-guided surgery

2016 ◽  
Vol 41 (6) ◽  
pp. 1173 ◽  
Author(s):  
Nikolas Dimitriadis ◽  
Bartłomiej Grychtol ◽  
Lars Maertins ◽  
Tobias Behr ◽  
George Themelis ◽  
...  
Keyword(s):  
Real Time ◽  
Imaging Method ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery

Real-Time Dynamic Visualization of Wheel Ruts Based on Vega Prime

2013 ◽  
Author(s):  
Wang Xuelong ◽  
Chi Ruijuan ◽  
Zhu Zhongxiang ◽  
Mao Enrong
Keyword(s):  
Real Time ◽  
Dynamic Visualization ◽  
Time Dynamic

Ultrasound-based real-time neuronavigated fluorescence-guided surgery for high-grade gliomas: technical note and preliminary experience

2019 ◽  
Vol 161 (12) ◽  
pp. 2595-2605
Author(s):  
Alessandro Villa ◽  
Gabriele Costantino ◽  
Francesco Meli ◽  
Antonino Odierna Contino ◽  
Alessia Imperato ◽  
...  
Keyword(s):  
Real Time ◽  
Technical Note ◽  
High Grade ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
High Grade Gliomas

scholarly journals NIMG-12. MULTICOLOURED-ASSISTED SURGERY FOR RESECTION OF HIGH GRADE GLIOMAS WITH 5-AMINOLEVULINACID

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi164-vi164
Author(s):  
Cleopatra Charalampaki ◽  
Dimitrios Atanasopoulos
Keyword(s):  
Real Time ◽  
White Light ◽  
High Grade ◽  
Field Orientation ◽  
Additional Information ◽  
Guided Surgery ◽  
Surgical Microscope ◽  
Fluorescence Guided Surgery ◽  
Surgical Field ◽  
High Grade Gliomas

Abstract INTRODUCTION Fluorescence guided surgery with the use of 5-aminolevulinacid (5 -ALA) is nowadays the state of the art treatment of high grade gliomas. However, intraoperative visualization of 5-ALA under dark blue light remains challenging, especially when leaking blood covers parts of the surgical field, where tumor fluorescence becomes invisible. To overcome this problem and combine the brightness of visible light with the information delivered by fluorescence we developed multispectral fluorescence, a technique able to project both information in real time to the surgeon’s eyes, and embedded it on a surgical microscope. METHODS We examined 40 patients with brain tumors who underwent surgery, using the MFL operative technique. The tumors involved comprised of 20 glioblastomas, 7 astrocytomas WHO III, 5 meningiomas and 8 metastatic tumors. The MFL technique with the real-time overlay of 5-ALA fluorescence and white light was intraoperatively compared to the classic blue filter detecting 5-ALA fluorescence. RESULTS The tumor was in all cases clearly visible and highlighted from the surrounding tissues. The pseudocolor we chose to visualize the tumor was green, representing 5-ALA fluorescence, with the surrounding brain tissueremained in its origin white color. In the cases where blood was covering the surgical field, orientation was easy to maintain, due to the additional information delivered from the white light image. CONCLUSIONS The MFL technique embedded on a surgical microscope opens the way for a precise and clear visualization of tumor 5-ALA fluorescence in real time under white light. It can be easily implemented in the resection of all tumors accumulating 5-ALA. Drawbacks of classic 5-ALA fluorescence such as hidden fluorescence because of overlying blood could be overcome with the presence of additional white light and the clear visualization of the exposed brain.

Real-time near-infrared fluorescence guided surgery in gynecologic oncology: A review of the current state of the art

2014 ◽  
Vol 135 (3) ◽  
pp. 606-613 ◽  
Author(s):  
Henricus J.M. Handgraaf ◽  
Floris P.R. Verbeek ◽  
Quirijn R.J.G. Tummers ◽  
Leonora S.F. Boogerd ◽  
Cornelis J.H. van de Velde ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
State Of The Art ◽  
Gynecologic Oncology ◽  
Near Infrared Fluorescence ◽  
Guided Surgery ◽  
Current State ◽  
Fluorescence Guided Surgery

Optical imaging probes and their potential contribution to radiotracer development

2016 ◽  
Vol 55 (02) ◽  
pp. 51-62 ◽  
Author(s):  
S. Hermann ◽  
M. Schäfers ◽  
C. Höltke ◽  
A. Faust
Keyword(s):  
Optical Imaging ◽  
Fluorescent Dyes ◽  
Great Influence ◽  
Potential Contribution ◽  
Preclinical Evaluation ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Imaging Probes ◽  
Unspecific Binding

SummaryOptical imaging has long been considered a method for histological or microscopic investigations. Over the last 15 years, however, this method was applied for preclinical molecular imaging and, just recently, was also able to show its principal potential for clinical applications (e.g. fluorescence-guided surgery). Reviewing the development and preclinical evaluation of new fluorescent dyes and target-specific dye conjugates, these often show characteristic patterns of their routes of excretion and biodistribution, which could also be interesting for the development and optimization of radiopharmaceuticals. Especially ionic charges show a great influence on biodistribution and netcharge and charge-distribution on a conjugate often determines unspecific binding or background signals in liver, kidney or intestine, and other organs.Learning from fluorescent probe behaviour in vivo and translating this knowledge to radio-pharmaceuticals might be useful to further optimize emerging and existing radiopharmaceuticals with respect to their biodistribution and thereby availability for binding to their targets.

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