Optical imaging probes and their potential contribution to radiotracer development

2016 ◽  
Vol 55 (02) ◽  
pp. 51-62 ◽  
Author(s):  
S. Hermann ◽  
M. Schäfers ◽  
C. Höltke ◽  
A. Faust
Keyword(s):  
Optical Imaging ◽  
Fluorescent Dyes ◽  
Great Influence ◽  
Potential Contribution ◽  
Preclinical Evaluation ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Imaging Probes ◽  
Unspecific Binding

SummaryOptical imaging has long been considered a method for histological or microscopic investigations. Over the last 15 years, however, this method was applied for preclinical molecular imaging and, just recently, was also able to show its principal potential for clinical applications (e.g. fluorescence-guided surgery). Reviewing the development and preclinical evaluation of new fluorescent dyes and target-specific dye conjugates, these often show characteristic patterns of their routes of excretion and biodistribution, which could also be interesting for the development and optimization of radiopharmaceuticals. Especially ionic charges show a great influence on biodistribution and netcharge and charge-distribution on a conjugate often determines unspecific binding or background signals in liver, kidney or intestine, and other organs.Learning from fluorescent probe behaviour in vivo and translating this knowledge to radio-pharmaceuticals might be useful to further optimize emerging and existing radiopharmaceuticals with respect to their biodistribution and thereby availability for binding to their targets.

Recent advances in molecular imaging probes for β-amyloid plaques

MedChemComm ◽  
2015 ◽  
Vol 6 (3) ◽  
pp. 391-402 ◽  
Author(s):  
Masahiro Ono ◽  
Hideo Saji
Keyword(s):  
Molecular Imaging ◽  
Optical Imaging ◽  
Amyloid Plaques ◽  
Recent Advances ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Β Amyloid ◽  
The Brain

We review recent advances in our development of molecular imaging probes for PET, SPECT, and optical imaging for in vivo detection of β-amyloid plaques in the brain.

scholarly journals Optical imaging-guided cancer therapy with fluorescent nanoparticles

2009 ◽  
Vol 7 (42) ◽  
pp. 3-18 ◽  
Author(s):  
Shan Jiang ◽  
Muthu Kumara Gnanasammandhan ◽  
Yong Zhang
Keyword(s):  
Cancer Therapy ◽  
Optical Imaging ◽  
Critical Role ◽  
Therapeutic Agents ◽  
Fluorescent Nanoparticles ◽  
Highly Sensitive ◽  
Imaging Probes ◽  
Recent Developments

The diagnosis and treatment of cancer have been greatly improved with the recent developments in nanotechnology. One of the promising nanoscale tools for cancer diagnosis is fluorescent nanoparticles (NPs), such as organic dye-doped NPs, quantum dots and upconversion NPs that enable highly sensitive optical imaging of cancer at cellular and animal level. Furthermore, the emerging development of novel multi-functional NPs, which can be conjugated with several functional molecules simultaneously including targeting moieties, therapeutic agents and imaging probes, provides new potentials for clinical therapies and diagnostics and undoubtedly will play a critical role in cancer therapy. In this article, we review the types and characteristics of fluorescent NPs, in vitro and in vivo imaging of cancer using fluorescent NPs and multi-functional NPs for imaging-guided cancer therapy.

Engineering microglia as intraoperative optical imaging agent vehicles potentially for fluorescence-guided surgery in gliomas

2020 ◽  
Vol 8 (4) ◽  
pp. 1117-1126
Author(s):  
Ling Guo ◽  
Xiaochen Zhang ◽  
Runxiu Wei ◽  
Gaojie Li ◽  
Bingzhi Sun ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Brain Tumors ◽  
Optical Imaging ◽  
Imaging Agent ◽  
Tumor Surgery ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
The Brain

Carotid artery injection of DiDBV2-Fe into glioblastoma-bearing mice and rats potentially for fluorescence-guided tumor surgery leads to significant accumulation and retention by the brain tumors.

scholarly journals Near-Infrared Molecular Imaging of Glioblastoma by Miltuximab®-IRDye800CW as a Potential Tool for Fluorescence-Guided Surgery

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 984 ◽  
Author(s):  
Dmitry M. Polikarpov ◽  
Douglas H. Campbell ◽  
Lucinda S. McRobb ◽  
Jiehua Wu ◽  
Maria E. Lund ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Near Infrared ◽  
Ex Vivo ◽  
Extent Of Resection ◽  
Fluorescent Imaging ◽  
Control Group ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Specific Accumulation

Glioblastoma (GBM) is one of the most aggressive tumors and its 5-year survival is approximately 5%. Fluorescence-guided surgery (FGS) improves the extent of resection and leads to better prognosis. Molecular near-infrared (NIR) imaging appears to outperform conventional FGS, however, novel molecular targets need to be identified in GBM. Proteoglycan glypican-1 (GPC-1) is believed to be such a target as it is highly expressed in GBM and is associated with poor prognosis. We hypothesize that an anti-GPC-1 antibody, Miltuximab®, conjugated with the NIR dye, IRDye800CW (IR800), can specifically accumulate in a GBM xenograft and provide high-contrast in vivo fluorescent imaging in rodents following systemic administration. Miltuximab® was conjugated with IR800 and intravenously administered to BALB/c nude mice bearing a subcutaneous U-87 GBM hind leg xenograft. Specific accumulation of Miltuximab®-IR800 in subcutaneous xenograft tumor was detected 24 h later using an in vivo fluorescence imager. The conjugate did not cause any adverse events in mice and caused strong fluorescence of the tumor with tumor-to-background ratio (TBR) reaching 10.1 ± 2.8. The average TBR over the 10-day period was 5.8 ± 0.6 in mice injected with Miltuximab®-IR800 versus 2.4 ± 0.1 for the control group injected with IgG-IR800 (p = 0.001). Ex vivo assessment of Miltuximab®-IR800 biodistribution confirmed its highly specific accumulation in the tumor. The results of this study confirm that Miltuximab®-IR800 holds promise for intraoperative fluorescence molecular imaging of GBM and warrants further studies.

scholarly journals TMOD-16. MULTIFUNCTIONAL NANOPARTICLES USED FOR MAGNETIC RESONANCE/NIR FLUORESCENCE DUAL-MODAL IMAGING AND CHEMOTHERAPY OF GLIOMA

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi266-vi266
Author(s):  
Shiguang Zhao
Keyword(s):  
Fluorescence Imaging ◽  
Average Diameter ◽  
Diagnostic Methods ◽  
Survival Times ◽  
Multifunctional Nanoparticles ◽  
Guided Surgery ◽  
Therapeutic Drugs ◽  
Fluorescence Guided Surgery

Abstract PURPOSE Glioma is the most common malignant brain tumor and infamous for its high invasiveness and lethality. Therefore, plenty of techniques like intraoperative MRI (iMRI) and fluorescence-guided surgery were performed to improve the surgical resection rate of glioma. In addition, the chemotherapy effect of glioma was severely restricted because of the low blood-brain barrier (BBB) permeability of traditional therapeutic drugs. Doxorubicin (DOX) is a widely used antineoplastic drug, but its application in glioma therapy is prevented by the BBB. Thus, it is urgent to develop novel diagnostic methods and drug delivery systems to improve glioma treatment effect. MATERIALS AND METHODS In this study, we constructed a multifunctional nanotheranostic agent which integrated MR imaging, fluorescence imaging and targeting chemotherapy for glioma by encapsulating iron oxide nanoparticles, DOX and indocyanine green (ICG) with PEGylated phospholipid. RESULTS The obtained multifunctional were proved with an average diameter of 22.9 nm. In vitro cell experiments demonstrated that the nanoparticle effectively enhanced cellular uptake of DOX compared with that of free DOX. In vivo MR and fluorescence imaging not only demonstrated that the nanotheranostic agent effectively crossed the BBB and selectively accumulated at the tumor area, but also easily monitored the real-time therapeutic outcome. Among all treatment groups, C6 glioma-bearing rats treated with nanoparticles exhibited the maximal degree of efficacy, including the longest survival times and smallest tumor volume. CONCLUSION All these results suggested that the multifunctional nanoparticles could be simultaneously used for iMRI and fluorescence-guided surgery to increase the resection rates, as well as delivering chemotheraputic drugs to tumor site to improve therapeutic effect. We believe that this multifunctional nanoparticle might serve as a potential theranostic treatment for glioma in the near future.

scholarly journals One-pot synthesis of water-soluble near-infrared fluorescence RNase A capped CuInS2 quantum dots for in vivo imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (80) ◽  
pp. 50949-50954 ◽  
Author(s):  
Yue Xi ◽  
Jianjun Yang ◽  
Yunshen Ge ◽  
Shenli Zhao ◽  
Jianguang Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Near Infrared ◽  
Rnase A ◽  
Water Soluble ◽  
One Pot ◽  
Guided Surgery ◽  
Nir Fluorescence ◽  
Fluorescence Guided Surgery ◽  
Cuins2 Quantum Dots

Near-infrared (NIR) quantum dots (QDs) have been treated as a promising candidate of imaging agents for NIR fluorescence-guided surgery. Here, the RNase A-CuInS2 QDs is good candidate, which performers well in gastrointestinal system imaging.

scholarly journals Dual Color Imaging from a Single BF2-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification

2020 ◽  
Author(s):  
Niamh Curtin ◽  
Dan Wu ◽  
Ronan A. Cahill ◽  
Anwesha Sarkar ◽  
Pól Mac Aonghusa ◽  
...  
Keyword(s):  
Lymph Node ◽  
Fluorescence Imaging ◽  
Color Imaging ◽  
Lymph Node Mapping ◽  
Guided Surgery ◽  
Dual Color ◽  
Fluorescence Guided Surgery ◽  
Clinical Systems

<p>Dual color fluorescence imaging has been achieved in the highly complex biomedical scenario of lymph node mapping. Emissions at 700 and 800 nm can be achieved from a single fluorophore by establishing molecular and aggregate forms. Fluorophore was compatible with clinical systems for fluorescence guided surgery and no toxicity was observed in high dosage testing.</p>

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