A biocompatible dual-AIEgen system without spectral overlap for quantitation of microbial viability and monitoring of biofilm formation

<p>Lacking rapid and reliable microbial detection and sensing platforms, insufficient understanding towards microbial behaviors may generated or delayed precautions could be made, which greatly threatens human life and increase heavy financial burdens to the society. In this contribution, a dual-aggregation-induced emission luminogen (AIEgen) system is successfully developed for microbial imaging and metabolic status sensing. This system consists of two AIEgens (DCQA and TPE-2BA) that bear positive charged groups or boric acid groups, providing universal microbial staining ability and specific affinity to dead microbes, respectively. On the basis of the distinctive fluorescence response produced by the diverse interaction of AIEgens with live or dead microbes, this dual-AIEgen system can detect all the microbe and identify their viabilities. Furthermore, the morphology and metabolic status of a sessile biofilm can also be imaged and monitored. The system exhibits rapid labelling, suitable for various microbes, and good biocompatibilities.</p>

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A Biocompatible Dual-AIEgen System without Spectral Overlap for Quantitation of Microbial Viability and Monitoring of Biofilm Formation

10.26434/chemrxiv.13269617.v1 ◽

2020 ◽

Author(s):

Wei He ◽

Zheng Zheng ◽

Haotian Bai ◽

Ling-Hong Xiong ◽

Lei Wang ◽

...

Keyword(s):

Boric Acid ◽

Biofilm Formation ◽

Human Life ◽

Metabolic Status ◽

Fluorescence Response ◽

Aggregation Induced Emission ◽

Microbial Detection ◽

Sensing Platforms ◽

Specific Affinity ◽

Financial Burdens

<p>Lacking rapid and reliable microbial detection and sensing platforms, insufficient understanding towards microbial behaviors may generated or delayed precautions could be made, which greatly threatens human life and increase heavy financial burdens to the society. In this contribution, a dual-aggregation-induced emission luminogen (AIEgen) system is successfully developed for microbial imaging and metabolic status sensing. This system consists of two AIEgens (DCQA and TPE-2BA) that bear positive charged groups or boric acid groups, providing universal microbial staining ability and specific affinity to dead microbes, respectively. On the basis of the distinctive fluorescence response produced by the diverse interaction of AIEgens with live or dead microbes, this dual-AIEgen system can detect all the microbe and identify their viabilities. Furthermore, the morphology and metabolic status of a sessile biofilm can also be imaged and monitored. The system exhibits rapid labelling, suitable for various microbes, and good biocompatibilities.</p>

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Reduced virulence and enhanced host adaption during antibiotics therapy: A story of a within-host carbapenem-resistant Klebsiella pneumoniae sequence type 11 evolution in a fatal scrotal abscess patient

10.1101/2021.05.06.443049 ◽

2021 ◽

Author(s):

Meiping Ye ◽

Chunjie Liao ◽

Mengya Shang ◽

Danyang Zou ◽

Jingmin Yan ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Binding Site ◽

Biofilm Formation ◽

Capsular Polysaccharide ◽

Human Life ◽

Sequence Type ◽

Genomic Diversity ◽

Rna Seq ◽

Carbapenem Resistant ◽

Virulence Attenuation

Carbapenem-resistant Klebsiella pneumoniae (CRKP) has disseminated globally and become a major threat to human life. The sequence type (ST) 11 CRKP is a dominant clone in Asia, especially China, but how this clone evolves in vivo, then adapts to host and facilitates dissemination remain largely unknown. We analyzed the genomic dynamics of 4 ST11-CRKP isolates sequencially isolated from the urine of a patient with initial fatal scrotal abscess and finally recovered without effective medication. Genomic differences were identified and their implications for pathogenesis and host adaptation were investigated. The related transcriptional pathways were further explored by RNA-Seq. Genomic analysis identified 4-24 mutations and 94%-100% were synonymous or intergenic. The mutation rate of ST11-CRKP was 2.1×10-6-1.7×10-5 substitutions/site/year over 47 days of antibiotics therapy. During this period, CRKP underwent several adaptive changes including tigecycline resistance and virulence attenuation. Tigecycline resistance was caused by ramR ribosomal binding site (RBS) deletion, which has been described by us previously. In this study, we demonstrated that mutations associated with acyltransferase (act) and ompK26 caused the virulence attenuation of ST11-CRKP. act deletion reduced the production of capsular polysaccharide and enhanced biofilm formation. RNA-Seq analysis revealed that act influenced the expression of ldhA, bglX, mtnK and metE which likely participate in capsular synthesis and biofilm formation. ompK26 affected the virulence by its overexpression caused by the deletion of upstream repressor binding site. Our finding suggested that the broad genomic diversity, high evolutionary capacity and rapid within-host adaptability of ST11-CRKP might contribute to the worldwide dissemination of this clone.

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Bacterial biofilm behavior in water-supply lines of dental units

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124811 ◽

1992 ◽

Vol 50 (1) ◽

pp. 882-883

Author(s):

B.D. Tall ◽

K.S. George ◽

R. T. Gray ◽

H.N. Williams

Keyword(s):

Water Supply ◽

Medical Devices ◽

Biofilm Formation ◽

Bacterial Biofilm

Studies of bacterial behavior in many environments have shown that most organisms attach to surfaces, forming communities of microcolonies called biofilms. In contaminated medical devices, biofilms may serve both as reservoirs and as inocula for the initiation of infections. Recently, there has been much concern about the potential of dental units to transmit infections. Because the mechanisms of biofilm formation are ill-defined, we investigated the behavior and formation of a biofilm associated with tubing leading to the water syringe of a dental unit over a period of 1 month.

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Biosecurity: tools, behaviours and concepts

Emerging Topics in Life Sciences ◽

10.1042/etls20200343 ◽

2020 ◽

Vol 4 (5) ◽

pp. 449-452

Author(s):

Alan MacLeod ◽

Nicola Spence

Keyword(s):

Plant Protection ◽

Human Life ◽

Animal Health ◽

Early Warning Systems ◽

Policy Makers ◽

Recent Developments ◽

Surveillance Programs ◽

Harmful Organisms ◽

The One ◽

Biosecurity Practices

COVID 19 has raised the profile of biosecurity. However, biosecurity is not only about protecting human life. This issue brings together mini-reviews examining recent developments and thinking around some of the tools, behaviours and concepts around biosecurity. They illustrate the multi-disciplinary nature of the subject, demonstrating the interface between research and policy. Biosecurity practices aim to prevent the spread of harmful organisms; recognising that 2020 is the International Year of Plant Health, several focus on plant biosecurity although invasive species and animal health concerns are also captured. The reviews show progress in developing early warning systems and that plant protection organisations are increasingly using tools that compare multiple pest threats to prioritise responses. The bespoke modelling of threats can inform risk management responses and synergies between meteorology and biosecurity provide opportunities for increased collaboration. There is scope to develop more generic models, increasing their accessibility to policy makers. Recent research can improve pest surveillance programs accounting for real-world constraints. Social science examining individual farmer behaviours has informed biosecurity policy; taking a broader socio-cultural approach to better understand farming networks has the potential to change behaviours in a new way. When encouraging public recreationists to adopt positive biosecurity behaviours communications must align with their values. Bringing together the human, animal, plant and environmental health sectors to address biosecurity risks in a common and systematic manner within the One Biosecurity concept can be achieved through multi-disciplinary working involving the life, physical and social sciences with the support of legislative bodies and the public.

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