Ultra-low background signaling cascade amplifier for in vivo fluorescence imaging of hydroxyl radical production induced by testosterone

2021 ◽  
Author(s):  
wenjie zhao ◽  
Yanxia Nan ◽  
Yu Gu ◽  
Qiulan Zhou ◽  
Jun Zhang
Keyword(s):  
Hydroxyl Radical ◽  
Fluorescence Imaging ◽  
Signaling Cascade ◽  
Crucial Importance ◽  
Analyte Concentration ◽  
Low Background ◽  
Radical Production ◽  
In Vivo Detection ◽  
In Vivo Fluorescence Imaging

The designing of cascade assay strategies for sensing ultralow analyte concentration is of crucial importance. However, the conventional strategies are not fit for in vivo detection due to the need...

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging

2011 ◽  
Vol 16 (9) ◽  
pp. 096013 ◽  
Author(s):  
Julien Gravier ◽  
Fabrice P. Navarro ◽  
Thomas Delmas ◽  
Frédérique Mittler ◽  
Anne-Claude Couffin ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

In vivo fluorescence imaging of glutamate in sensory cortex

2010 ◽  
Vol 68 ◽  
pp. e155
Author(s):  
Hiroshi Sekiya ◽  
Shigeyuki Namiki ◽  
Hirokazu Sakamoto ◽  
Sho Iinuma ◽  
Kenzo Hirose ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Sensory Cortex ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared Region

2012 ◽  
Vol 51 (39) ◽  
pp. 9818-9821 ◽  
Author(s):  
Guosong Hong ◽  
Joshua T. Robinson ◽  
Yejun Zhang ◽  
Shuo Diao ◽  
Alexander L. Antaris ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Infrared Region ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging ◽  
Near Infrared Region ◽  
Ag2s Quantum Dots

Tracing of islet graft survival by way of in vivo fluorescence imaging

2011 ◽  
Vol 27 (6) ◽  
pp. 575-583 ◽  
Author(s):  
Sevim Kahraman ◽  
Ercument Dirice ◽  
Fatma Z. Hapil ◽  
Mustafa G. Ertosun ◽  
Saffet Ozturk ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Graft Survival ◽  
Islet Graft ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

In vivo fluorescence imaging for cancer diagnosis using receptor-targeted epidermal growth factor-based nanoprobe

Biomaterials ◽  
2013 ◽  
Vol 34 (36) ◽  
pp. 9149-9159 ◽  
Author(s):  
Ju Hee Ryu ◽  
Miyoung Shin ◽  
Sun Ah Kim ◽  
Sangmin Lee ◽  
Hyunjoon Kim ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Fluorescence Imaging ◽  
Cancer Diagnosis ◽  
In Vivo Fluorescence ◽  
Epidermal Growth ◽  
In Vivo Fluorescence Imaging

Dual-Activator Codoped Upconversion Nanoprobe with Core–Multishell Structure for in Vitro and in Vivo Detection of Hydroxyl Radical

2017 ◽  
Vol 89 (20) ◽  
pp. 11021-11026 ◽  
Author(s):  
Xinyue Song ◽  
Jiayu Zhang ◽  
Zihong Yue ◽  
Zonghua Wang ◽  
Zhihong Liu ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
In Vivo Detection

scholarly journals MMP-14-targeted Nanoprobe for in vivo Fluorescence Imaging of Rupture-prone Carotid Plaques

2021 ◽  
Author(s):  
Mengtao Han ◽  
Kaining Liu ◽  
Hongqiu Xiao ◽  
Tao Sun ◽  
Fei Wang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Signal ◽  
Plaque Progression ◽  
Carotid Plaques ◽  
In Vivo Fluorescence ◽  
Cell Tissue ◽  
A Cell ◽  
In Vivo Fluorescence Imaging ◽  
Membrane Anchoring

Abstract Background: The identification of rupture-prone carotid plaques for preventing stroke remains a clinical challenge. Macrophage matrix metalloproteinase (MMP)-14, which contributes to plaque progression and destabilisation, could be a promising biomarker for plaque imaging. This study aimed to design and synthesise an MMP-14-targeted nanoprobe to noninvasively visualise the behaviour of M1 macrophages in atherosclerotic plaques.Methods: A fluorescence molecular imaging probe ([email protected]) was constructed by covalently attaching the fluorescent dye cyanine (Cy) 5.5, an MMP-14 substrate, and polyethylene glycol (PEG) 5000-wrapped gold nanoparticles (AuNPs), and then administered via tail vein injection to carotid atherosclerosis models for in vivo fluorescence imaging. Additionally, carotid tissues and cultured macrophages were analysed for nanoprobe binding, and MMP-14 and inflammation-related marker expression was evaluated by polymerase chain reaction, western blotting, and immunohistochemistry.Results: MMP-14 expression significantly increased with plaque progression, along with the upregulation of MMP-2 and inflammatory M1 markers, CD68 and F4/80, and significant downregulation of the M2 marker CD206. All of cell, tissue and in vivo fluorescence imaging exhibited a favourable targeting efficacy of [email protected] for MMP-14.Conclusions: MMP-14, a cell membrane-anchoring enzyme, can serve as a biomarker of vulnerable plaques, and MMP-14 substrate-based [email protected], with an intense fluorescence signal after activation and good biocompatibility, can be applied to screen for and monitor plaque progression in vivo.

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