pH-responsive Pt-based nanoradiosensitizer for enhanced radiotherapy via oxidative stress amplification

Tumor radioresistance is a major issue in radiotherapy. To address it, a pH-responsive nanoradiosensitizer was synthesized employing a simple method. Initially, chloroplatinic acid was reduced by human serum albumin (HSA)...

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Allysine and α-Aminoadipic Acid as Markers of the Glyco-Oxidative Damage to Human Serum Albumin under Pathological Glucose Concentrations

Antioxidants ◽

10.3390/antiox10030474 ◽

2021 ◽

Vol 10 (3) ◽

pp. 474 ◽

Cited By ~ 1

Author(s):

Carolina Luna ◽

Alexis Arjona ◽

Carmen Dueñas ◽

Mario Estevez

Keyword(s):

Oxidative Stress ◽

Plasma Concentration ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Typical Feature ◽

Advanced Glycation ◽

Chemical Mechanisms ◽

Fasting Plasma ◽

Insight Into

Understanding the molecular basis of the disease is of the utmost scientific interest as it contributes to the development of targeted strategies of prevention, diagnosis, and therapy. Protein carbonylation is a typical feature of glyco-oxidative stress and takes place in health disorders such as diabetes. Allysine as well as its oxidation product, the α-amino adipic acid (α-AA) have been found to be markers of diabetes risk whereas little is known about the chemistry involved in its formation under hyperglycemic conditions. To provide insight into this issue, human serum albumin was incubated in the presence of FeCl3 (25 μM) and increasing glucose concentrations for 32 h at 37 °C. These concentrations were selected to simulate (i) physiological fasting plasma concentration (4 mM), (ii) pathological pre-diabetes fasting plasma concentration (8 mM), and pathological diabetes fasting plasma concentration (12 mM) of glucose. While both allysine and α-AA were found to increase with increasing glucose concentrations, the carboxylic acid was only detected at pathological glucose concentrations and appeared to be a more reliable indicator of glyco-oxidative stress. The underlying chemical mechanisms of lysine glycation as well as of the depletion of tryptophan and formation of fluorescent and colored advanced glycation products are discussed.

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Stabilizing mechanisms in commercial albumin preparations: octanoate and N-acetyl-l-tryptophanate protect human serum albumin against heat and oxidative stress

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2004.07.002 ◽

2004 ◽

Vol 1702 (1) ◽

pp. 9-17 ◽

Cited By ~ 57

Author(s):

Makoto Anraku ◽

Yasufumi Tsurusaki ◽

Hiroshi Watanabe ◽

Toru Maruyama ◽

Ulrich Kragh-Hansen ◽

...

Keyword(s):

Oxidative Stress ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

And Oxidative Stress

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Cys34-Cysteinylated Human Serum Albumin Is a Sensitive Plasma Marker in Oxidative Stress-Related Chronic Diseases

PLoS ONE ◽

10.1371/journal.pone.0085216 ◽

2014 ◽

Vol 9 (1) ◽

pp. e85216 ◽

Cited By ~ 74

Author(s):

Kohei Nagumo ◽

Motohiko Tanaka ◽

Victor Tuan Giam Chuang ◽

Hiroko Setoyama ◽

Hiroshi Watanabe ◽

...

Keyword(s):

Oxidative Stress ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Chronic Diseases ◽

Plasma Marker

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Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

10.20944/preprints201702.0070.v1 ◽

2017 ◽

Author(s):

Ypatios Spanidis ◽

Alexandros Priftis ◽

Dimitrios Stagos ◽

George A. Stravodimos ◽

Demetres D. Leonidas ◽

...

Keyword(s):

Oxidative Stress ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Reduction Potential ◽

Individual Variability ◽

Protein Carbonyls ◽

Marathon Race ◽

Oxidation Reduction ◽

Oxidation Reduction Potential

The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress after an exhaustive exercise such as ultra-marathon race. Thus, blood samples from 12 adult runners who underwent a 103 km mountain ultra-marathon race were collected pre- and 24, 48 and 72 h post race. HSA was partially purified using affinity chromatography and then was subjected to Western blot analysis for disulfide dimers determination, indicating oxidation. The results were correlated with those from a previous study in which the same samples were analyzed using different oxidative stress markers and a good correlation with protein carbonyls (PC) at all time points was observed. Moreover there was a significant correlation with static oxidation-reduction potential (sORP) at 24 h, and a negative correlation with capacity oxidation-reduction potential (cORP) at 24 and 48 h. In addition, an individual analysis of albumin dimers exhibited great inter-individual differences. This inter-individual variability in the oxidation of HSA may suggest different interventions (e.g. through diet) in order to confront the effects on athletes’ organism after a strenuous exercise. In conclusion, this study supported the importance of the assessment of albumin dimers as a predictive marker for exercise-induced oxidative stress.

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