Exploring divalent conjugates of 5-N-acetyl-neuraminic acid as inhibitors of coxsackievirus A24 variant (CVA24v) transduction

Eye Infection ◽

Coxsackievirus A24 variant (CVA24v) is responsible for several outbreaks and two pandemics of the highly contagious eye infection acute hemorrhagic conjunctivitis (AHC).

Phylogenetic and molecular characterization of coxsackievirus A24 variant isolates from a 2010 acute hemorrhagic conjunctivitis outbreak in Guangdong, China

Virology Journal ◽

10.1186/1743-422x-9-41 ◽

2012 ◽

Vol 9 (1) ◽

pp. 41 ◽

Cited By ~ 14

Author(s):

Wu De ◽

Zheng Huanying ◽

Li Hui ◽

Monagin Corina ◽

Guo Xue ◽

...

Keyword(s):

Molecular Characterization ◽

Epidemics of Acute Hemorrhagic Conjunctivitis by Coxsackievirus A24 Variant

10.1159/000417544 ◽

2015 ◽

pp. 57-65

Author(s):

Marguerite Yin-Murphy

Keyword(s):

Outbreak of acute hemorrhagic conjunctivitis in French Guiana and West Indies caused by coxsackievirus A24 variant: Phylogenetic analysis reveals Asian import

Journal of Medical Virology ◽

10.1002/jmv.20304 ◽

2005 ◽

Vol 75 (4) ◽

pp. 559-565 ◽

Cited By ~ 33

Author(s):

Philippe Dussart ◽

Gaëlle Cartet ◽

Pierre Huguet ◽

Nicolas Lévêque ◽

Christian Hajjar ◽

...

Keyword(s):

Phylogenetic Analysis ◽

West Indies ◽

French Guiana ◽

Rapid Identification of the Coxsackievirus A24 Variant by Molecular Serotyping in an Outbreak of Acute Hemorrhagic Conjunctivitis

Journal of Clinical Microbiology ◽

10.1128/jcm.43.3.1069-1071.2005 ◽

2005 ◽

Vol 43 (3) ◽

pp. 1069-1071 ◽

Cited By ~ 10

Author(s):

S.-W. Park ◽

C.-S. Lee ◽

H.-C. Jang ◽

E.-C. Kim ◽

M.-d. Oh ◽

...

Keyword(s):

Rapid Identification ◽

Molecular Serotyping ◽

Molecular identification of coxsackievirus A24 variant, isolated from an outbreak of acute hemorrhagic conjunctivitis in Singapore in 2005

Archives of Virology ◽

10.1007/s00705-007-1032-z ◽

2007 ◽

Vol 152 (11) ◽

pp. 2005-2016 ◽

Cited By ~ 16

Author(s):

D. S.-Y. Yeo ◽

S. G.-K. Seah ◽

J. S.-W. Chew ◽

E. A.-S. Lim ◽

J. C.-W. Liaw ◽

...

Keyword(s):

Molecular Identification ◽

Molecular identification and phylogenetic study of coxsackievirus A24 variant isolated from an outbreak of acute hemorrhagic conjunctivitis in India in 2010

Archives of Virology ◽

10.1007/s00705-012-1520-7 ◽

2012 ◽

Vol 158 (3) ◽

pp. 679-684 ◽

Cited By ~ 10

Author(s):

Deepti Shukla ◽

Arvind Kumar ◽

Shalini Srivastava ◽

Tapan N. Dhole

Keyword(s):

Molecular Identification ◽

Phylogenetic Study ◽

Exploring the Effect of Structure-Based Scaffold Hopping on the Inhibition of Coxsackievirus A24v Transduction by Pentavalent N-Acetylneuraminic Acid Conjugates

International Journal of Molecular Sciences ◽

10.3390/ijms22168418 ◽

2021 ◽

Vol 22 (16) ◽

pp. 8418

Author(s):

Emil Johansson ◽

Rémi Caraballo ◽

Daniel L. Hurdiss ◽

Nitesh Mistry ◽

C. David Andersson ◽

...

Keyword(s):

Antiviral Agents ◽

Host Cells ◽

Scaffold Hopping ◽

Corneal Epithelial ◽

Acetylneuraminic Acid ◽

Design And Synthesis ◽

Human Corneal Epithelial Cells ◽

Eye Infection ◽

Coxsackievirus A24 variant (CVA24v) is the primary causative agent of the highly contagious eye infection designated acute hemorrhagic conjunctivitis (AHC). It is solely responsible for two pandemics and several recurring outbreaks of the disease over the last decades, thus affecting millions of individuals throughout the world. To date, no antiviral agents or vaccines are available for combating this disease, and treatment is mainly supportive. CVA24v utilizes Neu5Ac-containing glycans as attachment receptors facilitating entry into host cells. We have previously reported that pentavalent Neu5Ac conjugates based on a glucose-scaffold inhibit CVA24v infection of human corneal epithelial cells. In this study, we report on the design and synthesis of scaffold-replaced pentavalent Neu5Ac conjugates and their effect on CVA24v cell transduction and the use of cryogenic electron microscopy (cryo-EM) to study the binding of these multivalent conjugates to CVA24v. The results presented here provide insights into the development of Neu5Ac-based inhibitors of CVA24v and, most significantly, the first application of cryo-EM to study the binding of a multivalent ligand to a lectin.