Enzymic hydrolysis of sphingolipids. Hydrolysis of ceramide lactoside by an enzyme from rat brain

Ceramide lactoside [1-O-(galactosido-4-beta-glucosido)-2-N-acyl-sphingosine] was hydrolysed to ceramide glucoside and galactose by beta-galactosidase of rat brain. The reaction was not reversible, required cholate or taurocholate, had optimum pH5.0 and K(m) 2.2x10(-5)m. It was inhibited by gamma-galactonolactone and galactose as well as by ceramide, sphingosine and fatty acid. Ceramide lactoside could be degraded to ceramide, galactose and glucose by mixtures of rat-brain beta-galactosidase and ox-brain beta-glucosidase.

Download Full-text

Hydrolysis of dietary flavonoid glycosides by strains of intestinal Bacteroides from humans

Biochemical Journal ◽

10.1042/bj2480953 ◽

1987 ◽

Vol 248 (3) ◽

pp. 953-956 ◽

Cited By ~ 194

Author(s):

V D Bokkenheuser ◽

C H Shackleton ◽

J Winter

Keyword(s):

Healthy Subjects ◽

Enzymic Activity ◽

Flavonoid Glycosides ◽

Faecal Flora ◽

A Cell ◽

Bacteroides Ovatus ◽

Dietary Flavonoid ◽

Beta Glucosidase ◽

Hydrolysis Of ◽

Beta Galactosidase

Rutin and quercitrin are hydrolysed to quercetin, and robinin is hydrolysed to kaempferol, by faecal flora from healthy subjects. The enzymes required for these hydrolyses, namely alpha-rhamnosidase and beta-galactosidase, were produced by some strains of Bacteroides distasonis; other strains, however, synthesized beta-glucosidase. The last-named enzyme was also elaborated by Bacteroides uniformis and Bacteroides ovatus. All the enzymes were produced constitutively. A cell-free extract of B. distasonis containing beta-glucosidase displayed an enzymic activity of 1 mumol/10 min per 10 mg of protein.

Download Full-text

Enzymic hydrolysis of arachidonoyl-phospholipids by rat brain synaptosomes

Neurochemistry International ◽

10.1016/0197-0186(85)90038-5 ◽

1985 ◽

Vol 7 (5) ◽

pp. 825-831 ◽

Cited By ~ 21

Author(s):

Judith A. Kelleher ◽

Grace Y. Sun

Keyword(s):

Rat Brain ◽

Enzymic Hydrolysis ◽

Rat Brain Synaptosomes ◽

Brain Synaptosomes ◽

Hydrolysis Of

Download Full-text

Enzymic hydrolysis of sphingolipids. Hydrolysis of ceramide glucoside by an enzyme from ox brain

Biochemical Journal ◽

10.1042/bj1010687 ◽

1966 ◽

Vol 101 (3) ◽

pp. 687-691 ◽

Cited By ~ 52

Author(s):

G Shimon

Keyword(s):

Palmitic Acid ◽

Acetate Buffer ◽

Enzymic Hydrolysis ◽

Ionic Detergent ◽

Anionic Detergents ◽

Beta Glucosidase ◽

Triton X ◽

Hydrolysis Of

Ceramide glucoside (1-O-glucosido-2-N-acyl-sphingosine) was hydrolysed to ceramide (N-acyl-sphingosine) and glucose by beta-glucosidase from ox brain. The reaction was stimulated by the non-ionic detergent, Triton X-100, or by the anionic detergents, cholate or taurocholate. It was not reversible, had optimum pH5.0 (with acetate buffer) or 5.6 (with pyridine buffer), had K(m) 1.8x10(-4)m and was inhibited by delta-gluconolactone and sphingosine, but not by ceramide or palmitic acid.

Download Full-text