scholarly journals Radioautographic studies of the initial site of formation of protein-bound iodine in the rat thyroid gland

1970 ◽  
Vol 118 (2) ◽  
pp. 311-314 ◽  
Author(s):  
C. J. Croft ◽  
Rosalind Pitt-Rivers
Keyword(s):  
Thyroid Gland ◽  
Peripheral Portion ◽  
Thyroid Glands ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

1. Predominantly cellular labelling was observed in radioautographs of rat thyroid glands fixed by perfusion from the aorta at intervals between 5 and 55s after [125I]iodide administration via the aorta. 2. When perfusion was delayed for 2min, or if immersion fixation was used, the labelling was predominantly over the peripheral portion of the follicular lumen, in agreement with the observations of other investigators. 3. The findings support the concept that the initial site of binding of iodine to protein is intracellular, but the nature of this protein has not been established.

INABILITY OF HETEROLOGOUS ANTIBODIES TO AFFECT RAT THYROID GLAND

1961 ◽  
Vol 39 (4) ◽  
pp. 691-694 ◽  
Author(s):  
H. S. Sodhi
Keyword(s):  
Thyroid Gland ◽  
Thyroid Antibodies ◽  
Specific Antibodies ◽  
Thyroid Glands ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

Heterologous rat thyroid antibodies produced in rabbits were injected by intraperitoneal, intravenous, and intra-arterial routes in different groups of rats and the effects on the morphology and 24-hour I131 uptake of their thyroid glands were investigated. In spite of the administration of high titers of specific antibodies no effects, acute or chronic, were observed, indicating the inability of the heterologous thyroid antibodies to alter the structure or function of the rat thyroid glands.

IODINATED THYROLIPIDS: THEIR POSSIBLE ROLE IN HORMONOGENESIS

1973 ◽  
Vol 74 (3) ◽  
pp. 461-474 ◽  
Author(s):  
D. H. Shah ◽  
U. R. Thakare ◽  
R. C. Shownkeen ◽  
D. N. Pahuja ◽  
M. Y. Mandlik
Keyword(s):  
Thyroid Gland ◽  
Lipid Fraction ◽  
Human Thyroid ◽  
Cystic Degeneration ◽  
Nodular Goitre ◽  
Thyroid Glands ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

ABSTRACT A total of 42 human thyroid glands (nodular goitre 9, adenoma with cystic degeneration 6; toxic goitre 10; carcinoma 14; and normal thyroid gland 3) were examined in vitro for iodination of an unidentified polar non-phosphatide lipid fraction (fraction II). The radioiodine incorporation in fraction II was 49.3 %, 43.6 %, 32.8 %, 20.7 %, and 22.0 % respectively in normal, nodular goitre, adenoma with cystic degeneration, toxic goitre and thyroid carcinoma. In vitro studies with surviving sheep thyroid slices did not show any relation between the iodination of fraction II and thyroxine formation over a period of 120 min. However, a highly significant correlation (r-value= 0.96375) was observed between the iodination of fraction II and thyroxine formation in vivo in the rat thyroid gland over a period of 24 h. We have previously postulated that iodination of fraction II may be interrelated to thyroxine formation. In the light of this hypothesis and the above results we suggest that the iodination of fraction II and thyroxine formation in the thyroid gland may be interrelated, the degree of iodination of fraction II being modulated by the amount of thyroxine formed within the thyroid gland.

Functional development and maturation of the rat thyroid gland in the foetal and newborn periods: an immunohistochemical study

1985 ◽  
Vol 108 (4) ◽  
pp. 518-524 ◽  
Author(s):  
Akira Kawaoi ◽  
Minoru Tsuneda
Keyword(s):  
Thyroid Gland ◽  
Immunohistochemical Study ◽  
Neonatal Rat ◽  
Immunohistochemical Detection ◽  
Functional Development ◽  
Significant Difference ◽  
Thyroid Glands ◽  
Rat Thyroid ◽  
Thyroid Epithelium ◽  
Rat Thyroid Gland

Abstract. Immunohistochemical detection of T4, T3 and thyroglobulin (Tg) was undertaken in foetal and neonatal rat thyroid glands in an attempt to elucidate the functional development and maturation of the gland during these periods. Immunoreactive Tg first appeared in the cytoplasm of the immature thyroid epithelium on the 15th day of gestation, followed by the occurrence of T4 and T3 in the lumen of primitive follicles of the thyroid gland 2 days later. The stainability of Tg, T4 and T3 increased as the follicular structure became mature; however, no significant difference in staining patterns was observed during the perinatal periods.

Effects of toxic doses of a novel histamine (H2) antagonist on the rat thyroid gland

1987 ◽  
Vol 25 (10) ◽  
pp. 787-794 ◽  
Author(s):  
C.G. Brown ◽  
R.F. Harland ◽  
I.R. Major ◽  
C.K. Atterwill
Keyword(s):  
Thyroid Gland ◽  
H2 Antagonist ◽  
Histamine H2 Antagonist ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

CORRELATION OF IODIDE TRANSPORT WITH Na+, K+ -ATPase, HCO−3-ATPase AND CARBONIC ANHYDRASE ACTIVITIES IN DIFFERENT FUNCTIONAL STATES OF THE RAT THYROID GLAND

1982 ◽  
Vol 92 (3) ◽  
pp. 371-379 ◽  
Author(s):  
S.-Y. CHOW ◽  
J. W. KEMP ◽  
D. M. WOODBURY
Keyword(s):  
Thyroid Gland ◽  
Carbonic Anhydrase ◽  
Protein Content ◽  
Chronic Treatment ◽  
Cell Water ◽  
Iodide Uptake ◽  
G Cell ◽  
Functional States ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

The effects of thyrotrophin, hypophysectomy, and chronic treatment with thyroxine and methimazole on radioiodide uptake (thyroid/plasma (T/P) 125I− ratio), protein and DNA contents and activities of Na+,K+ -ATPase, HCO−3-ATPase, and carbonic anhydrase (CA) of rat thyroid gland were evaluated. Thyrotrophin given to intact rats slightly increased thyroid iodide uptake, did not affect protein or DNA content, and slightly inhibited CA activity (units/g cell water). Hypophysectomy markedly decreased T/P 125I− ratio, increased protein content, decreased activity of Na+,K+-ATPase, and slightly increased HCO−3-ATPase (nmol/mg DNA per min) and CA (units/g cell water) activities. Thyro-trophin given to hypophysectomized rats (as compared with untreated hypophysectomized control animals) markedly increased T/P 125I− ratio, slightly decreased protein content and decreased Na+,K+-ATPase and CA activities. Chronic treatment with methimazole increased T/P 125I− ratio, decreased protein content, markedly increased Na+,K+-ATPase and HCO−3-ATPase activities, and decreased CA activity. Chronic treatment with thyroxine, in contrast, decreased T/P 125I− ratio, decreased Na+,K+-ATPase activity, and increased CA activity. There was a significant inverse correlation between T/P 125I− ratio and CA activity in follicular cells for the various induced functional states of the thyroid.

scholarly journals ENGLISH VERSION: AGE FEATURES OF RADIOACTIVE IODINE (131I) ABSORPTION BY RAT THYROID GLANDS IN CORRECTION OF THE DIETARY IODINE DEFICIENCY WITH ORGANIC IODINE

2021 ◽  
Vol 25 (1-2) ◽  
pp. 31-35
Author(s):  
O.I. Ryabukha
Keyword(s):  
Thyroid Gland ◽  
Iodine Deficiency ◽  
Radioactive Iodine ◽  
Male Rats ◽  
Organic Iodine ◽  
Study Results ◽  
Thyroid Glands ◽  
Immature Rats ◽  
Old Rats ◽  
Rat Thyroid

The structure of endocrine morbidity is characterized by a significant spread of thyroid pathology. The insufficient efficacy of inorganic iodine drugs poses the problem of search for new means for iodine deficiency treatment and prevention. Given the progressive aging of the population in economically developed countries, the purpose of the study was to clarify the effect of organic iodine on the features of absorption and elimination of radioactive iodine from the thyroid glands of variously aged rats in the conditions of iodine deficiency in the diet. The study was performed on nonlinear white male rats in two series of studies that were kept on iodine-deficient isocaloric starch-casein diet for 60 days: the first series included two groups of old rats weighing 0.400-0.450 kg, the second series – two groups of sexually immature rats weighing 0.060-0.090 kg. There were 5 rats in each group. In animals of the experimental groups in each series, 10% of casein in the diet was replaced with organic iodine, which came with iodine-protein preparation from the red Black Sea algae Phyllophora nervosa. The functional state of the thyroid gland was studied using the Sodium Iodide Na 131 I Injection drug. The dosimetry was performed using the STS-6 Geiger-Muller Detector. Radioindication of the thyroid gland was carried out after subcutaneous administration of 0.1 ml of 131I solution at the following time intervals: 0.5, 1, 2, 4, 6, 8, 10, 12, 24, 48, 72, and 96 hours after administration of 131I. The study results were presented as a percentage of the radioiodine dose administered, adjusted for natural radioactivity background and the radioactive decay of the drug. It was found that in the iodine deficiency conditions, the thyroid glands of old rats have higher rates of radioiodine absorption and a lower rate of its excretion than the glands of immature rats, which indicates their lower iodine reserve and greater liability to iodine deficiency pathology. Intake of organic iodine regardless of the rats’ age is accompanied by a decrease in radioiodine accumulation and acceleration of its excretion from the thyroid gland, which indicates a decrease in functional stress, but the glands of older rats absorb more iodine and excrete it more slowly, indicating less effective correction of iodine deficiency with age. Reduced functional activity of the thyroid glands in old rats can be used as a sensitive changes marker for the in-depth study of thyrotropic and thyroid disrupting effects.

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