scholarly journals Uptake and utilization of 5'-methylthioadenosine by cultured baby-hamster kidney cells

1982 ◽  
Vol 204 (3) ◽  
pp. 803-807 ◽  
Author(s):  
T O Eloranta ◽  
K Tuomi ◽  
A M Raina
Keyword(s):  
Nucleic Acids ◽  
Cellular Metabolism ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Purine Nucleotide ◽  
Salvage Pathway ◽  
Baby Hamster Kidney Cells

5'-Methylthioadenosine was taken up and immediately metabolized further by cultured baby-hamster kidney cells during the exponential phase of growth. The adenine moiety supplied the purine-nucleotide pool via the salvage pathway and was efficiently incorporated into nucleic acids. Catabolites of methylthioadenosine excreted by the cells included adenine, purinic compounds and metabolites of the ribose portion. 5'-Methylthiotubercidin had no significant effect on the cellular metabolism of methyl-thioadenosine, but greatly inhibited its uptake. erythro-9-(2-Hydroxy-3-nonyl)adenine had no effect on the uptake, but markedly interfered with the further utilization of methylthioadenosine after cleavage in the cells.

scholarly journals Synthesis of ribonucleic acid in baby-hamster kidney cells in the presence of actinomycin D

1967 ◽  
Vol 105 (3) ◽  
pp. 979-985 ◽  
Author(s):  
S J Martin ◽  
F. Brown
Keyword(s):  
Ribonucleic Acid ◽  
Base Composition ◽  
Actinomycin D ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Rich Medium ◽  
Prolonged Incubation ◽  
Rna Molecules ◽  
Baby Hamster Kidney Cells

1. RNA molecules with sedimentation values in sucrose gradients of 12–20s are synthesized in baby-hamster kidney cells even after prolonged incubation in medium containing 1μg. of actinomycin D/ml. 2. The rate of formation of this RNA is dependent on the age of the cultures and is greatest during the exponential phase of growth. 3. Growth of cells on nutritionally poor medium causes degradation and inhibits the synthesis of these RNA fractions. 4. Replacement of the nutritionally poor medium with a rich medium stimulates the synthesis of actinomycin-resistant RNA. This stimulation is blocked by cycloheximide. 5. The base composition of this RNA is characterized by low cytidine and high guanosine values.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

scholarly journals Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica.

1997 ◽  
Vol 41 (10) ◽  
pp. 2286-2288 ◽  
Author(s):  
S Ankri ◽  
T Miron ◽  
A Rabinkov ◽  
M Wilchek ◽  
D Mirelman
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Kidney Cells ◽  
Cysteine Proteinases ◽  
Baby Hamster Kidney ◽  
Important Contributor ◽  
Cytopathic Effects ◽  
Baby Hamster Kidney Cells

The ability of Entamoeba histolytica trophozoites to destroy monolayers of baby hamster kidney cells is inhibited by allicin, one of the active principles of garlic. Cysteine proteinases, an important contributor to amebic virulence, as well as alcohol dehydrogenase, are strongly inhibited by allicin.

scholarly journals Cytotoxicity of Pristine C<sub>60</sub> Fullerene on Baby Hamster Kidney Cells in Solution

2012 ◽  
Vol 03 (03) ◽  
pp. 385-390 ◽  
Author(s):  
Shufang Liu ◽  
Haijie Liu ◽  
Zhijuan Yin ◽  
Kai Guo ◽  
Xibao Gao
Keyword(s):  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Baby Hamster Kidney Cells

scholarly journals Clonal growth of hamster free alveolar cells in soft agar.

1975 ◽  
Vol 142 (4) ◽  
pp. 877-886 ◽  
Author(s):  
H S Lin ◽  
C Kuhn ◽  
T Kuo
Keyword(s):  
Clonal Growth ◽  
Soft Agar ◽  
Culture Conditions ◽  
Mononuclear Phagocytes ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Alveolar Cells ◽  
Bronchial Washing ◽  
Lag Period ◽  
Baby Hamster Kidney Cells

Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes were found in the colony. Medium conditioned by baby hamster kidney cells or other cells was required for the initiation and maintenance of their growth. Alveolar cells from normal mice and rats also formed colonies under appropriate culture conditions.

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