scholarly journals Synthesis of ribonucleic acid in baby-hamster kidney cells in the presence of actinomycin D

1967 ◽  
Vol 105 (3) ◽  
pp. 979-985 ◽  
Author(s):  
S J Martin ◽  
F. Brown
Keyword(s):  
Ribonucleic Acid ◽  
Base Composition ◽  
Actinomycin D ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Rich Medium ◽  
Prolonged Incubation ◽  
Rna Molecules ◽  
Baby Hamster Kidney Cells

1. RNA molecules with sedimentation values in sucrose gradients of 12–20s are synthesized in baby-hamster kidney cells even after prolonged incubation in medium containing 1μg. of actinomycin D/ml. 2. The rate of formation of this RNA is dependent on the age of the cultures and is greatest during the exponential phase of growth. 3. Growth of cells on nutritionally poor medium causes degradation and inhibits the synthesis of these RNA fractions. 4. Replacement of the nutritionally poor medium with a rich medium stimulates the synthesis of actinomycin-resistant RNA. This stimulation is blocked by cycloheximide. 5. The base composition of this RNA is characterized by low cytidine and high guanosine values.

scholarly journals Uptake and utilization of 5'-methylthioadenosine by cultured baby-hamster kidney cells

1982 ◽  
Vol 204 (3) ◽  
pp. 803-807 ◽  
Author(s):  
T O Eloranta ◽  
K Tuomi ◽  
A M Raina
Keyword(s):  
Nucleic Acids ◽  
Cellular Metabolism ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Purine Nucleotide ◽  
Salvage Pathway ◽  
Baby Hamster Kidney Cells

5'-Methylthioadenosine was taken up and immediately metabolized further by cultured baby-hamster kidney cells during the exponential phase of growth. The adenine moiety supplied the purine-nucleotide pool via the salvage pathway and was efficiently incorporated into nucleic acids. Catabolites of methylthioadenosine excreted by the cells included adenine, purinic compounds and metabolites of the ribose portion. 5'-Methylthiotubercidin had no significant effect on the cellular metabolism of methyl-thioadenosine, but greatly inhibited its uptake. erythro-9-(2-Hydroxy-3-nonyl)adenine had no effect on the uptake, but markedly interfered with the further utilization of methylthioadenosine after cleavage in the cells.

scholarly journals Thermal stability of ribosomal ribonucleic acid from baby hamster kidney cells

1966 ◽  
Vol 101 (3) ◽  
pp. 721-726 ◽  
Author(s):  
SJ Martin
Keyword(s):  
Heat Treatment ◽  
Thermal Stability ◽  
Ribonucleic Acid ◽  
Sedimentation Coefficient ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Similar Treatment ◽  
Ribosomal Ribonucleic Acid ◽  
Thermal Stability Of ◽  
Baby Hamster Kidney Cells

1. RNA has been prepared from baby hamster kidney cells by extraction with a phenol-EDTA mixture and further purified by passing through a column of Sephadex G-25 that had been equilibrated with water. 2. Aging of the total RNA extracts at 4 degrees or heating at 95 degrees followed by rapid cooling caused a conversion of 28s RNA into material sedimenting in sucrose gradients at approx. 18s. 3. When heated RNA was re-extracted with phenol the sedimentation profile was not returned to that of the unheated RNA. 4. The 28s and 18s RNA fractions were collected separately from sucrose gradients by precipitation with 2vol. of ethanol and passed through a Sephadex G-25 column equilibrated with water. 5. Heat treatment of purified 28s RNA at 95 degrees caused the sedimentation coefficient to increase to approx. 40s, whereas similar treatment of 18s RNA caused no significant increase. If the RNA was heated before the Sephadex G-25 treatment the sedimentation coefficient of the 28s and 18s RNA decreased to approx. 12s and 8s. 6. Heating mixtures of purified 28s and 18s RNA at 95 degrees caused some aggregation of 18s material with the 28s fraction.

scholarly journals Effect of virus infection on the stability and synthesis of actinomycin-resistant ribonucleic acid in baby-hamster kidney cells

1967 ◽  
Vol 105 (3) ◽  
pp. 987-993 ◽  
Author(s):  
S J Martin ◽  
F. Brown
Keyword(s):  
Base Composition ◽  
Rna Synthesis ◽  
Sucrose Gradient ◽  
Foot And Mouth Disease ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Mouth Disease Virus ◽  
Infected Cells ◽  
The Stability ◽  
Baby Hamster Kidney Cells

1. The sucrose-gradient pattern of 32P-labelled RNA synthesized in actinomycintreated baby-hamster kidney cells infected with foot-and-mouth-disease virus depends greatly on the period of labelling. 2. Fractions are formed in infected cells that sediment at 12–20s and have the same base composition as similar fractions found in non-infected cells that have been treated with actinomycin. 3. In the presence of guanidine, which completely inhibits viral RNA synthesis, these fractions are labelled to a greater extent than in non-infected cells.

Sign in / Sign up

Export Citation Format

Share Document