Phosphoenolpyruvate carboxykinase from guinea-pig liver mitochondria. Immunological evidence for increase in enzyme amount during neonatal development

Phosphoenolpyruvate carboxykinase was purified from mitochondria of guinea-pig liver by affinity chromatography on GMP-Sepharose. The enzyme was purified 100-fold to a high degree of electrophoretic homogeneity as judged by detection of a single protein band on sodium dodecyl sulphate/polyacrylamide gels. The yield was about 16%. The Mr of the purified enzyme was estimated to be 68500 +/- 680 by analysis on sodium dodecyl sulphate/polyacrylamide gels. Antibodies raised in rabbits against the purified enzyme were highly specific for mitochondrial phosphoenolpyruvate carboxykinase and did not precipitate the cytosolic form of this enzyme from either rat or guinea-pig liver cytosol. The use of this antibody showed that starvation does not increase the amount of the enzyme. However, neonatal-development-dependent increase in its activity is shown to be mediated by accumulation of phosphoenol pyruvate carboxykinase-specific protein.

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THE REDUCTION OF l-XYLULOSE TO XYLITOL BY GUINEA PIG LIVER MITOCHONDRIA

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)65182-8 ◽

1956 ◽

Vol 221 (2) ◽

pp. 697-709 ◽

Cited By ~ 9

Author(s):

Oscar Touster ◽

V.H. Reynolds ◽

Ruth M. Hutcheson

Keyword(s):

Guinea Pig ◽

Liver Mitochondria ◽

Pig Liver ◽

Guinea Pig Liver

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The transglutaminase in vascular cells and tissues could provide an alternate pathway for fibrin stabilization

Blood ◽

10.1182/blood.v70.3.702.bloodjournal703702 ◽

1987 ◽

Vol 70 (3) ◽

pp. 702-709

Author(s):

CS Greenberg ◽

KE Achyuthan ◽

MJ Borowitz ◽

MA Shuman

Keyword(s):

Guinea Pig ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Vascular Cells ◽

Pig Liver ◽

Alternate Pathway ◽

Sds Page ◽

Immunohistochemical Techniques ◽

Plasmin Inhibitor ◽

Guinea Pig Liver

A thrombin-independent transglutaminase (TG) has been identified in vascular cells and tissues from human, rabbit, rat, porcine, and bovine sources. The vascular TG had several properties that were similar but not identical to guinea pig liver TG. Both enzymes had similar chromatographic and electrophoretic properties, preferentially cross- linked the alpha-chains of fibrinogen, and reacted with polyclonal and monoclonal anti-guinea-pig liver TG antibodies. However, the TG from adult bovine aortic endothelial (ABAE) cells exhibited a novel Ca2+/Mg2+ dependence for enzymatic activity that was distinct from that of purified guinea pig liver TG. The mol wt of the vascular TG (79 +/- 3 kd) determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was slightly lower than the purified guinea pig liver TG (85 +/- 9 kd). The TG antigen was detected by immunohistochemical techniques in association with the endothelial and smooth muscle cells of arteries, veins, venules, and capillaries. The TG antigen also codistributed with the fibronectin antigen along the hepatic sinusoids. The ABAE cell TG cross-linked alpha 2-plasmin inhibitor to fibrinogen and caused the modified fibrinogen to be 40- fold more resistant to plasminolysis. A thrombin-independent TG in vascular cells of blood vessels could provide an alternate pathway to inhibit fibrinolysis and promote fibrin stabilization.

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Transport of ascorbate into guinea pig liver mitochondria

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(82)90044-x ◽

1982 ◽

Vol 684 (1) ◽

pp. 21-26 ◽

Cited By ~ 24

Author(s):

Ole Christian Ingebretsen ◽

Per T. Normann

Keyword(s):

Guinea Pig ◽

Liver Mitochondria ◽

Pig Liver ◽

Guinea Pig Liver

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Development of fatty acid oxidation in neonatal guinea-pig liver

Biochemical Journal ◽

10.1042/bj2080723 ◽

1982 ◽

Vol 208 (3) ◽

pp. 723-730 ◽

Cited By ~ 6

Author(s):

D A Shipp ◽

M Parameswaran ◽

I J Arinze

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Guinea Pig ◽

Ketone Bodies ◽

Liver Mitochondria ◽

Fatty Acyl ◽

Acid Oxidation ◽

Beta Oxidation ◽

Pig Liver ◽

Guinea Pig Liver

The capacity of foetal and neonatal liver to oxidize short-, medium- and long-chain fatty acids was studied in the guinea pig. Liver mitochondria from foetal and newborn animals were unable to synthesize ketone bodies from octanoate, but octanoylcarnitine and palmitoylcarnitine were readily ketogenic. The ketogenic capacity at 24 h after birth was as high as in adult animals. Hepatocytes isolated from term animals were unable to oxidize fatty acids, but at 6 h after birth production of 14CO2, acid-soluble products and acetoacetate from 1-14C-labelled fatty acids was 40-50% of the rates at 24 h. At 12 h of age these rates had already reached the 24 h values and did not change during suckling in the first week of life. The activities of hepatic fatty acyl-CoA synthetases, which were minimal in the foetus or at term, increased to maximal values in 12-24 h. The data show that the capacity for beta-oxidation and ketogenesis develops maximally in this species during the first 6-12 h after birth, and appears to be partly dependent on the development of fatty acid-activating enzyme.

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