scholarly journals Molecular cloning of membrane cofactor protein (MCP; CD46) on B95a cell, an Epstein–Barr virus-transformed marmoset B cell line: B95a-MCP is susceptible to infection by the CAM, but not the Nagahata strain of the measles virus

1998 ◽  
Vol 330 (3) ◽  
pp. 1351-1359 ◽  
Author(s):  
Yusuke MURAKAMI ◽  
Tsukasa SEYA ◽  
Mitsue KURITA ◽  
Aya FUKUI ◽  
Shigeharu UEDA ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Measles Virus ◽  
Epstein Barr Virus ◽  
Vero Cells ◽  
Membrane Cofactor Protein ◽  
High Susceptibility ◽  
African Green Monkey Kidney ◽  
Barr Virus ◽  
Epstein Barr

Measles virus (MV) infects not only human beings but also some simian species. The MV receptor on Vero cells (a cell line established from African Green monkey kidney cells) and human cells has been shown to be the membrane cofactor protein MCP/CD46, which is an inhibitor of autologous complement (C) activation. B95a, an Epstein-Barr virus (EBV)-transformed marmoset B cell line, is a simian cell line used for MV selection and is much more susceptible to MV than Vero cells. In the present study, we isolated cDNAs encoding MCP homologues from B95a cDNA library and assessed whether B95a-MCP is responsible for the high susceptibility of B95a to MV. The deduced amino acid sequence of the cDNA of B95a-MCP was 76% identical to that of human-MCP, and the recombinant B95a-MCP exerts C inhibitor activity. Although CAM, a vaccine strain of MV, infected Chinese hamster ovary (CHO) cells expressing B95a-MCP, Nagahata strain, a wild type of MV, failed to infect the CHO transfectants, suggesting that additional membrane molecules of B95a are responsible for the high susceptibility of B95a to the Nagahata strain.

scholarly journals Signatures of B-cell receptor diversity in B lymphocytes following Epstein-Barr virus transformation

2019 ◽  
Vol 51 (6) ◽  
pp. 197-207
Author(s):  
Meimei Lai ◽  
Qiongdan Wang ◽  
Yutian Lu ◽  
Xi Xu ◽  
Ying Xia ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
High Throughput Sequencing ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Human Virus ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

Epstein-Barr virus (EBV) is a widespread human virus that establishes latent infection, potentially leading to tumors, hematological disorders, and other severe diseases. EBV infections are associated with diverse symptoms and affect various organs; therefore, early diagnosis and treatment are crucial. B cell receptor (BCR) repertoires of B cell surface immunoglobulins have been widely studied for their association with various infectious diseases. However, the specific genetic changes that modulate the BCR repertoires after an EBV infection are still poorly understood. In this study, we employed high-throughput sequencing (HTS) to investigate the diversity of BCR repertoires in an EBV-transformed lymphoblastic cell line (LCL). Compared with the noninfected control B cell line, the LCL exhibited a decrease in overall BCR diversity but displayed an increase in the expansion of some dominant rearrangements such as IGHV4-31/IGHJ4, IGHV4-59/IGHJ4, IGHV5-51/IGHJ3, and IGHV3-74/IGHJ3. A higher frequency of occurrence of these rearrangement types was confirmed in patients with EBV infection. Interestingly, the IGHV3-74 rearrangement was only detected in EBV-infected children, suggesting that our experimental observations were not coincidental. In addition, we identified a highly dominant consensus motif, CAR(xRx)YGSG(xYx)FD, in complementarity-determining region 3 (CDR3) sequences of the heavy chain in the LCL. Our findings demonstrated the utility of HTS technology for studying the variations in signature motifs of the BCR repertoires after EBV infection. We propose that the analysis of BCR repertoire sequences represents a promising method for diagnosing early EBV infections and developing novel antibody- and vaccine-based therapies against such infections.

Functional Analysis of an Epstein-Barr Virus-Nontransformed Human B Cell Line

1988 ◽  
Vol 80 (3) ◽  
pp. 139-144 ◽  
Author(s):  
Y. Yasuda ◽  
T. Iwasaki ◽  
T. Hamano ◽  
K. Nagai
Keyword(s):  
Functional Analysis ◽  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell

Bcl-2 and c-Myc co-operate in the Epstein – Barr virus-immortalized human B-cell line GM607 but do not confer tumorigenicity

2005 ◽  
Vol 46 (4) ◽  
pp. 581-592 ◽  
Author(s):  
Jennifer L Tomlin ◽  
Barbara-Ann Guinn ◽  
Linda Z Penn ◽  
Neil L Berinstein
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell

scholarly journals Lymphoma cell line (FL-18) and Epstein-Barr virus-carrying cell line (FL-18-EB) obtained from a patient with follicular lymphoma: monoclonal derivation and different properties

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1619-1623 ◽  
Author(s):  
S Doi ◽  
H Ohno ◽  
E Tatsumi ◽  
Y Arita ◽  
H Kamesaki ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Nuclear Antigen ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Barr Virus ◽  
Epstein Barr

Abstract A novel cell line, FL-18, was established from the pleural effusion of a patient with follicular small cleaved cell lymphoma. At the same time, an Epstein-Barr virus (EBV) nuclear antigen (EBNA)-positive cell line, FL-18-EB, was established from the EBV-infected culture of the same pleural effusion cells. Both cell lines had the same monoclonal surface immunoglobulin (IgG kappa), and they had the same karyotype as that of the fresh pleural effusion cells in which a reciprocal translocation between the long arm of chromosomes 14 and 18 [t(14;18)(q32;q21)] was detected. Gene rearrangement analysis of immunoglobulin heavy-chain gene (JH) and kappa light-chain gene (J kappa) showed the same rearranged configuration in the two cell lines; however, some morphological and phenotypic differences were found. The FL-18-EB cells, which were morphologically similar to common EBNA- positive lymphoblastoid cell lines of normal B cell origin at the initial phase of culture, were larger than the FL-18 cells and contained multinucleated giant cells. The FL-18 cells lacked cytoplasmic immunoglobulin and were positive for common acute lymphoblastic leukemia antigen (CALLA), whereas the FL-18-EB cells had cytoplasmic immunoglobulin and were negative for CALLA. Thus, the phenotype of FL-18-EB seems to be a result of a shift by EBV infection to a more mature stage in the B cell differentiation pathway than that of FL-18. The paired availability of EBV-free and EBV-infected cell lines of a neoplastic clone is unique and valuable in considering EBV infectibility of neoplastic B cells and resultant phenotypic changes.

Effects of sex steroids on immunoglobulin M production by Epstein-Barr virus-transformed B-cell line SKW6-CL4

1991 ◽  
Vol 8 (6) ◽  
pp. 329-335 ◽  
Author(s):  
Hidehiro Mori ◽  
Miho Sawairi ◽  
Naoki Itoh ◽  
Takahiro Hanabayashi ◽  
Teruhiko Tamaya
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Sex Steroids ◽  
Epstein Barr Virus ◽  
Immunoglobulin M ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr virus (EBV)-positive pyothorax-associated lymphoma (PAL): chromosomal integration of EBV in a novel CD2-positive PAL B-cell line

2002 ◽  
Vol 117 (3) ◽  
pp. 546-557 ◽  
Author(s):  
Masanori Daibata ◽  
Takahiro Taguchi ◽  
Yuiko Nemoto ◽  
Tsuyako Saito ◽  
Hisanori Machida ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Chromosomal Integration ◽  
Barr Virus ◽  
Epstein Barr
Sign in / Sign up

Export Citation Format

Share Document