Cytoplasmic free [Ca2+] is not increased in the platelets of deoxycorticosterone–salt and spontaneously hypertensive rats

1. The cytoplasmic free calcium concentration ([Ca2+]i) in the platelets of spontaneously hypertensive rats (SHR), Wistar–Kyoto rats (WKY), deoxycorticosterone–salt hypertensive rats (DOC) and normotensive Sprague–Dawley rats (SD) was measured with the fluorescent dye, quin-2-tetra-acetoxymethyl ester. 2. No significant difference in platelet [Ca2+]i was found between SHR and WKY or between DOC and SD rats. 3. No correlations were found between systolic blood pressure and [Ca2+]i. 4. These results suggest that the elevation of platelet [Ca2+]i does not necessarily accompany hypertension in rats.

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Effects of dietary salt on angiotensin peptides in kidney.

Journal of the American Society of Nephrology ◽

10.1681/asn.v641209 ◽

1995 ◽

Vol 6 (4) ◽

pp. 1209-1215

Author(s):

Q C Meng ◽

J Durand ◽

Y F Chen ◽

S Oparil

Keyword(s):

Angiotensin Ii ◽

Spontaneously Hypertensive Rats ◽

Spontaneously Hypertensive Rat ◽

Sprague Dawley ◽

Dietary Salt ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Angiotensin Peptides ◽

Sprague Dawley Rats ◽

Wistar Kyoto

This study used a novel simple method for the extraction, separation, identification, and quantitation of angiotensin-like immunoactivity from tissue to examine the effects of altering dietary NaCl intake on intrarenal angiotensin I, II, and III levels in salt-sensitive, spontaneously hypertensive rats, salt-resistant Wistar-Kyoto rats, and Sprague-Dawley rats. Seven-week-old male spontaneously hypertensive rats, Wistar-Kyoto rats, and Sprague-Dawley rats were assigned randomly to a diet containing either 8% (high) or 1% (basal) salt and were maintained on these diets for 3 wk. Rats were then decapitated without prior anesthesia, and kidneys were rapidly (< 30 s) removed, snap frozen in liquid nitrogen, and stored at -80 degrees C. Frozen tissue was extracted in 2 M acetic acid and then subjected to solid-phase extraction with the cation exchange resin AG 50W X4. Angiotensin peptides were separated by reversed-phase high-performance liquid chromatography on a phenyl silica gel column with an eluent consisting of 20% acetonitrile in 0.1 M ammonium phosphate buffer, pH 4.9, and quantitated by radioimmunoassay. The elution of standard peptides under isocratic conditions revealed clear resolution of angiotensin I, II, and III and the (1-7) and (3-8) peptides. Recoveries of both labeled and unlabeled angiotensin peptide standards from the extraction step were > 90%. Renal angiotensin II stores were significantly higher in spontaneously hypertensive rats than in Wistar-Kyoto or Sprague-Dawley rats, independent of diet. Renal angiotensin II and III were further suppressed during dietary salt supplementation in both salt-resistant strains but not in the spontaneously hypertensive rat. These findings are consistent with an enhanced (compared with Wistar-Kyoto and Sprague-Dawley rats) role for angiotensin II in the kidney of the salt-sensitive, spontaneously hypertensive rat, particularly under conditions of dietary salt supplementation.

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Cytoplasmic Free [Ca2+] is Increased in the Platelets of Spontaneously Hypertensive Rats and Essential Hypertensive Patients

Clinical Science ◽

10.1042/cs0680179 ◽

1985 ◽

Vol 68 (2) ◽

pp. 179-184 ◽

Cited By ~ 148

Author(s):

Giacomo Bruschi ◽

Maria E. Bruschi ◽

Maurizio Caroppo ◽

Guido Orlandini ◽

Marco Spaggiari ◽

...

Keyword(s):

Spontaneously Hypertensive Rats ◽

Calcium Concentration ◽

Free Calcium ◽

Cytoplasmic Calcium ◽

Hypertensive Rats ◽

Hypertensive Patients ◽

Spontaneously Hypertensive ◽

Human Control ◽

Free Calcium Concentration ◽

Wistar Kyoto

1. The cytoplasmic free calcium concentration ([Ca2+]i) was assessed with the fluorescent dye Quin 2 in platelets and lymphocytes of spontaneously hypertensive rats (SHR), normotensive Wistar—Kyoto rats (WKY), essential hypertensive patients (EHP) and normotensive human control subjects (NCS). 2. [Ca2+]i was significantly higher in the platelets of 8- and 20-week-old SHR in comparison with WKY. However, no difference was evident after weaning. Changes of cellular calcium in hypertensive rats apparently evolved simultaneously with the development of high arterial pressure. 3. [Ca2+]i was significantly higher in platelets of EHP than in NCS. 4. In lymphocytes of SHR, [Ca2+]i was not different from WKY at 4 and 8 weeks, but was increased at 14 weeks and at older ages. In EHP, intralymphocytic [Ca2+] was only modestly higher than in controls. On the whole, the results suggest that control of cytoplasmic calcium in these blood cells is similarly affected in human and animal models of primary hypertension.

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Sustained density of neuroendocrine cells with aging precedes development of prostatic hyperplasia in spontaneously hypertensive rats

BMC Urology ◽

10.1186/s12894-019-0528-7 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Yuki Kyoda ◽

Koji Ichihara ◽

Kohei Hashimoto ◽

Ko Kobayashi ◽

Fumimasa Fukuta ◽

...

Keyword(s):

Spontaneously Hypertensive Rats ◽

Prostatic Hyperplasia ◽

Neuroendocrine Cells ◽

Initial Growth ◽

Hypertensive Rats ◽

Initial Development ◽

Spontaneously Hypertensive ◽

Significant Difference ◽

The Difference ◽

Wistar Kyoto

Abstract Background Neuroendocrine (NE) cells may have an impact on the development and initial growth of benign prostatic hyperplasia (BPH) according to previous human studies. Methods To explore the relationship of NE cells and BPH development, we compared the density of NE cells and also prostatic weight in spontaneously hypertensive rats (SHR), which develop by aging, and Wistar-Kyoto rats (WKY) as control. The total weights of the epithelium and stroma in the ventral lobes of 8-, 12, 16-, 28- and 56-week-old SHR and WKY were calculated using Image J software. NE cells in the ventral prostatic ducts (VPd) were quantified using immunohistochemical staining for serotonin. Results Although there was no significant difference in the estimated total weight of the epithelium and stroma in the ventral lobes adjusted by body weight (ES weight) between the two groups at 8, 12 and 16 weeks of age, ES weight was significantly greater in the SHR group than in the WKT group at 28 and 56 weeks. The density of NE cells in the VPd decreased with aging in the WKY group, whereas it was sustained until 16 weeks and then decreased with aging in the SHR group. The difference in the density between the two groups was most marked at 16 weeks of age. Conclusion In the natural history of BPH, NE cells may play an important role in the initial development of BPH because sustained density of NE cells in the VPd precedes the development of prostatic hyperplasia.

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Comparing pharmacokinetics and metabolism of diltiazem in normotensive Sprague Dawley and Wistar Kyoto rats vs. spontaneously hypertensive rats in vivo

Drug Metabolism and Drug Interactions ◽

10.1515/dmdi.2011.012 ◽

2011 ◽

Vol 26 (3) ◽

Cited By ~ 1

Author(s):

Pollen K.F. Yeung ◽

Angelita Alcos ◽

Tanya Marcoux ◽

Jinglan Tang

Keyword(s):

Spontaneously Hypertensive Rats ◽

Sprague Dawley ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Wistar Kyoto Rats ◽

Wistar Kyoto

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Intra-Erythrocyte Sodium and (Na+,K+-Activated)-ATPase Concentration and Urinary Aldosterone Excretion in Spontaneously Hypertensive Rats

Clinical Science ◽

10.1042/cs0600229 ◽

1981 ◽

Vol 60 (2) ◽

pp. 229-232 ◽

Cited By ~ 13

Author(s):

G. Berglund ◽

L. Sigström ◽

S. Lundin ◽

B. E. Karlberg ◽

H. Herlitz

Keyword(s):

Spontaneously Hypertensive Rats ◽

Sodium Concentration ◽

Intracellular Sodium ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Wistar Kyoto Rats ◽

Significant Difference ◽

Intracellular Sodium Concentration ◽

Erythrocyte Sodium ◽

Wistar Kyoto

1. Intra-erythrocyte sodium, potassium, ATP and (Na+,K+-activated)-ATPase concentrations and urinary aldosterone excretion were compared in 3-month-old spontaneously hypertensive rats (n = 11) and normotensive Wistar-Kyoto control rats (n = 11). 2. Spontaneously hypertensive rats exhibited significantly higher intra-erythrocyte sodium concentration (5.5 ± 1.3 vs 4.0 ± 1.1 mmol/l of erythrocytes, P < 0.01). No significant difference was found in intra-erythrocyte potassium, ATP or (Na+,K+-activated)-ATPase concentration. 3. Mean urinary aldosterone excretion was significantly lower in spontaneously hypertensive rats (66.3 ± 6.5 pmol/24 h) than in Wistar-Kyoto rats (90.5 ± 10.6 pmol/24 h, P < 0.01). No significant relationship between urinary aldosterone and intra-erythrocyte sodium concentration was found in spontaneously hypertensive or Wistar-Kyoto rats or in the pooled group. 4. These results are thus consistent with previous findings of an increased intracellular sodium concentration in spontaneously hypertensive rats, but do not support the hypothesis that aldosterone is a dominant regulator of intracellular sodium concentration.

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