Altered l-arginine metabolism results in increased nitric oxide release from uraemic endothelial cells

2002 ◽  
Vol 103 (1) ◽  
pp. 31 ◽  
Author(s):  
Raj C. THURAISINGHAM ◽  
Norman B. ROBERTS ◽  
Mark WILKES ◽  
David I. NEW ◽  
A. Claudio MENDES-RIBEIRO ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Arginine Metabolism ◽  
Nitric Oxide Release

Altered l-arginine metabolism results in increased nitric oxide release from uraemic endothelial cells

2002 ◽  
Vol 103 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Raj C. THURAISINGHAM ◽  
Norman B. ROBERTS ◽  
Mark WILKES ◽  
David I. NEW ◽  
A. Claudio MENDES-RIBEIRO ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
In Vivo Model ◽  
No Production ◽  
Arginine Metabolism ◽  
Enos Expression ◽  
Nitric Oxide Release ◽  
Excessive Consumption ◽  
And Control

Results regarding the nitric oxide (NO) system in uraemia are contradictory. l-Arginine, the precursor of NO, is also metabolized by arginase to form ornithine and urea. In the present study, endothelial NO production and arginine metabolism in uraemia were assessed. In addition an in vivo model was used to examine excess consumption of NO in uraemia. NO and amino acid measurements were made from basal and stimulated (by bradykinin) uraemic and control endothelial cells. Reverse-transcriptase PCR was used to assess endothelial NO synthase (eNOS) and inducible NOS (iNOS) expression. Finally, aortae of uraemic rats were stained for nitrotyrosine (a marker of peroxynitrite). Basal uraemic cells produced more NO than the control cells. l-Arginine levels were greater in uraemic (supernatants/cells), but ornithine levels were higher in control (supernatants/cells). Following stimulation, NO levels in supernatants were similar, but the rise in NO production was greater in control compared with uraemic cells; l-arginine levels still remained higher in uraemic supernatants/cells. Differences in ornithine concentration (supernatants/cells) disappeared following bradykinin stimulation, due to a rise in ornithine levels in the uraemic group. There was no difference in eNOS expression, nor was iNOS detected in either group. Only aortae from uraemic rats showed evidence for nitrotyrosine staining. These studies demonstrated increased basal NO release in uraemic endothelial cells, perhaps by inhibition of arginase and hence diversion of arginine to the NO pathway. The increased NO produced under basal conditions may be inactive due to excessive consumption, resulting in peroxynitrite formation. Interestingly, bradykinin appears to restore arginase activity in uraemia, resulting in normalization of NO production.

Effects of substrate mechanics on angiogenic capacity and nitric oxide release in human endothelial cells

2020 ◽  
Vol 1470 (1) ◽  
pp. 31-43
Author(s):  
Atefeh Jannatbabaei ◽  
Mohammad Tafazzoli‐Shadpour ◽  
Ehsan Seyedjafari
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Nitric Oxide Release

EDF-1 contributes to the regulation of nitric oxide release in VEGF-treated human endothelial cells

2010 ◽  
Vol 89 (9) ◽  
pp. 654-660 ◽  
Author(s):  
Marzia Leidi ◽  
Massimo Mariotti ◽  
Jeanette A.M. Maier
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Nitric Oxide Release

Acetylcholine induces intracellular Ca2+ oscillations and nitric oxide release in mouse brain endothelial cells

Cell Calcium ◽  
2017 ◽  
Vol 66 ◽  
pp. 33-47 ◽  
Author(s):  
Estella Zuccolo ◽  
Dmitry Lim ◽  
Dlzar Ali Kheder ◽  
Angelica Perna ◽  
Paolo Catarsi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Mouse Brain ◽  
Brain Endothelial Cells ◽  
Nitric Oxide Release ◽  
Mouse Brain Endothelial Cells

Acetylcholine induces Ca2 + signals and nitric oxide release from human brain micrINS;ovascular endothelial cells

2018 ◽  
Vol 103-105 ◽  
pp. 65 ◽  
Author(s):  
E. Zuccolo ◽  
S. Negri ◽  
G. Pellavio ◽  
G. Scarpellino ◽  
U. Laforenza ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Human Brain ◽  
Nitric Oxide Release

Direct measurement of nitric oxide release from vascular endothelial cells

1996 ◽  
Vol 81 (2) ◽  
pp. 774-779 ◽  
Author(s):  
J. P. Guo ◽  
T. Murohara ◽  
M. Buerke ◽  
R. Scalia ◽  
A. M. Lefer
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Methyl Ester ◽  
Vascular Endothelial Cells ◽  
Calmodulin Antagonist ◽  
Nitric Oxide Release ◽  
A 23187 ◽  
No Release ◽  
Basal Release ◽  
Isolated Rat

A nitric oxide (NO)-selective electrode was used to directly measure NO release from isolated rat aortic endothelium and cultured rat aortic endothelial cells (RAECs). Basal release of NO was significantly attenuated by a NO synthase inhibitor NG-nitro-L-arginine methyl ester (1 mM) to 42 +/- 14 pmol/1 x 10(5) cells (P < 0.01). The basal release of NO was also significantly inhibited by a calmodulin antagonist W-7 at 15 microM (P < 0.01). L-Arginine (1 mM), significantly stimulated NO release (P < 0.05 vs. control basal release). Stimulation of cultured RAECs with two endothelium-dependent vasodilators, acetylcholine (100 nM) and A-23187 (1 microM), significantly increased NO release [574 +/- 112 pmol/1 x 10(5) cells (n = 5) and 658 +/- 119 pmol/1 x 10(5) cells (n = 5) in acetylcholine- and A-23187-stimulated RAECs, respectively]. Basal release of NO was also detectable in isolated rat aortic rings with intact endothelium. NO release was significantly attenuated by NG-nitro-L-arginine methyl ester and augmented by human superoxide dismutase. These data indicate the physiological usefulness of the amperometric measurement of NO employing a NO-specific electrode in biological systems.

Effect of D-glucose on nitric oxide release from glomerular endothelial cells

2001 ◽  
Vol 17 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Nobuhiko Kasai ◽  
Kazuhiro Sugimoto ◽  
Nobuo Horiba ◽  
Toshihiro Suda
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Release ◽  
Glomerular Endothelial Cells

Trans-resveratrol simultaneously increases cytoplasmic Ca2+ levels and nitric oxide release in human endothelial cells

2011 ◽  
Vol 55 (8) ◽  
pp. 1237-1248 ◽  
Author(s):  
Jacobo Elíes ◽  
Andrea Cuíñas ◽  
Verónica García-Morales ◽  
Francisco Orallo ◽  
Manuel Campos-Toimil
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Nitric Oxide Release
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