scholarly journals Detection of human cytomegalovirus (HCMV) pp67-mRIMA and pp65 antigenemia in relation to development of clinical HCMV disease in renal transplant recipients

2001 ◽  
Vol 7 (5) ◽  
pp. 254-260 ◽  
Author(s):  
I. Brook ◽  
M. Degré ◽  
K.I. Kristiansen ◽  
H. Rollag ◽  
E. Holter ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Human Cytomegalovirus ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Hcmv Disease ◽  
Pp65 Antigenemia

scholarly journals Human Cytomegalovirus-Encoded microRNAs Can Be Found in Saliva Samples from Renal Transplant Recipients

2020 ◽  
Vol 6 (4) ◽  
pp. 50
Author(s):  
Shelley Waters ◽  
Silvia Lee ◽  
Kylie Munyard ◽  
Ashley Irish ◽  
Patricia Price ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Immune Responses ◽  
Human Cytomegalovirus ◽  
Renal Transplant Recipients ◽  
Healthy Controls ◽  
Transplant Recipients ◽  
Host Immune Responses ◽  
Cell Responses ◽  
Hcmv Disease ◽  
Mirna Species

Human cytomegalovirus (HCMV) infections are common following renal transplantation and may have long-lasting effects. HCMV can be measured directly by viral DNA or indirectly via host immune responses. HCMV-encoded microRNA (miRNA) may alter the pathobiology of HCMV infections and contribute to the progression of HCMV disease. HCMV-encoded miRNAs can be detected in blood but have not been sought in saliva. We investigated saliva samples from 32 renal transplant recipients (RTR) and 12 seropositive healthy controls for whom immunological data was available. Five HCMV-encoded miRNAs (miR-UL112-5p, miR-US5-2-3p, miR-UL36, miR-US25-2-3p and miR-UL22A) were sought using primer probe assays. HCMV miRNA species were detected in saliva from 15 RTR and 3 healthy controls, with miR-US5-2-3p most commonly detected. The presence of HCMV miRNAs associated with increased T-cell responses to HCMV IE-1 in RTR, suggesting a link with frequent reactivations of HCMV.

scholarly journals Comparison of the CMV Brite Turbo Assay and the Digene Hybrid Capture CMV DNA (Version 2.0) Assay for Quantitation of Cytomegalovirus in Renal Transplant Recipients

2000 ◽  
Vol 38 (10) ◽  
pp. 3743-3745 ◽  
Author(s):  
Stephen K. N. Ho ◽  
Fu-Keung Li ◽  
Kar-Neng Lai ◽  
Tak-Mao Chan
Keyword(s):  
Renal Transplant ◽  
Turnaround Time ◽  
Small Sample ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Hybrid Capture ◽  
Pp65 Antigenemia ◽  
Version 2.0 ◽  
Blood Specimens ◽  
Cmv Disease

We compared the CMV Brite Turbo Kit (BT) and the Digene Hybrid Capture CMV DNA (version 2.0) assay (HC2) in the quantitation of pp65 antigenemia and cytomegalovirus (CMV) DNA levels in immunosuppressed renal transplant recipients. Of 123 blood specimens collected from 24 renal transplant recipients, BT and HC2 assays detected 35 and 39 positive samples, respectively. The overall concordance rate between the two assays was 90%. Discordant results were observed at low levels of viremia, so that 8 samples were HC2 positive but BT negative and another 4 were BT positive but HC2 negative. There was good correlation (R 2 = 0.766; P < 0.01) between the levels of CMV DNA and pp65 antigenemia in the 31 concordant positive samples. Correlation between results obtained with the two assays was confirmed by longitudinal studies for a patient who developed clinical CMV disease. HC2 may be more sensitive at low viremia levels and allow earlier detection of impending CMV disease. The BT assay offered the advantage of a rapid (2-h) turnaround time. We conclude that BT and HC2 assays have similar sensitivity and efficacy in the diagnosis and monitoring of CMV infection and disease in renal transplant recipients. While the HC2 assay would be appropriate for centers that handle a large number of samples, the BT test may be more suitable for small sample numbers or when results are needed urgently.

scholarly journals Human cytomegalovirus glycoprotein N polymorphisms among renal transplant recipients in India

2014 ◽  
Vol 14 (S3) ◽  
Author(s):  
A Raj Kumar Patro ◽  
Lalit Dar ◽  
Sunil K Pati ◽  
Sanjay K Agarwal ◽  
Sandeep Guleria ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Human Cytomegalovirus ◽  
Renal Transplant Recipients ◽  
Transplant Recipients

scholarly journals Human Cytomegalovirus DNA in Plasma and Serum Specimens of Renal Transplant Recipients Is Highly Fragmented

2002 ◽  
Vol 40 (11) ◽  
pp. 4105-4113 ◽  
Author(s):  
R. Boom ◽  
C. J. A. Sol ◽  
T. Schuurman ◽  
A. van Breda ◽  
J. F. L. Weel ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Human Cytomegalovirus ◽  
Renal Transplant Recipients ◽  
Transplant Recipients
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