scholarly journals The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69

2002 ◽  
Vol 28 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Simon J. Elliott ◽  
Leslie A. Wainwright ◽  
Timothy K. McDaniel ◽  
Karen G. Jarvis ◽  
YingKang Deng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Complete Sequence ◽  
Enteropathogenic Escherichia Coli ◽  
Enterocyte Effacement

scholarly journals Hierarchy in the expression of the locus of enterocyte effacement genes of enteropathogenic Escherichia coli

1999 ◽  
Vol 34 (5) ◽  
pp. 941-952 ◽  
Author(s):  
Devorah Friedberg ◽  
Tatiana Umanski ◽  
Yuan Fang ◽  
Ilan Rosenshine
Keyword(s):  
Escherichia Coli ◽  
Enteropathogenic Escherichia Coli ◽  
Enterocyte Effacement

scholarly journals Molecular Study of Regulatory Gene (Ler) in Enteropathogenic Escherichia Coli (EPEC) of Diarrheagenic Patients

2020 ◽  
pp. 2486-2493
Author(s):  
Mokhtar Jawad Kadhim AL-Imam ◽  
May Talib Flayyih
Keyword(s):  
Escherichia Coli ◽  
Regulatory Gene ◽  
Morphological Characteristics ◽  
Molecular Study ◽  
Enteropathogenic Escherichia Coli ◽  
Biochemical Tests ◽  
Secretion Pathway ◽  
Genes Expression ◽  
Vitek 2 ◽  
Enterocyte Effacement

The locus of enterocyte effacement LEE-encoded regulator (Ler( is a global regulator of multiple virulence genes expression in the Enteropathogenic Escherichia coli (EPEC), including those encoding the type III secretion pathway and adhesion proteins such as intimin. Ler is central to the process of the formation of the attaching and effacing (AE) lesions. This study aimed to perform the molecular detection of Ler gene in EPEC, since there is no related previous study in Iraq. Two hundred and fifty stool specimens from children under two years of age for both sexes were collected from some Iraqi hospitals. All isolates were diagnosed according to morphological characteristics and biochemical tests. The results showed that 140 (56%) samples were identified as E.coli, while 8  (5.7%) isolates were identified as EPEC as confirmed by using VITEK 2 system. Susceptibility test was determined for all EPEC isolates to 16 different antibiotics. The results showed that 100%of these isolates were resistant to Ampicillin, Cefazolin, Ceftriaxone, Cefepime, Trimethoprim and Ceftazidime, whereas resistance values to Nitrofurantoin, Cefoxitin and Gentamicin were  66%, 40%, and 15% respectively. However 100% of the isolates were  sensitive to Piperacillin, Ertapenem, Imipenem, Amikacin, Ciprofloxacin, Levofloxacin and Tigecycline. Monoplex pattern of PCR was used for detecting 16SrRNA, eae, stx1, lifA and Ler genes in EPEC. The results showed that the isolates of E.coli were positive for 16SrRNA, eae, lifA and Ler, while no bands of stx1 appeared.

CesAB is an enteropathogenic Escherichia coli chaperone for the type-III translocator proteins EspA and EspB

Microbiology ◽  
2003 ◽  
Vol 149 (12) ◽  
pp. 3639-3647 ◽  
Author(s):  
Elizabeth A. Creasey ◽  
Devorah Friedberg ◽  
Robert K. Shaw ◽  
Tatiana Umanski ◽  
Stuart Knutton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Interactions ◽  
Type Iii Secretion ◽  
Secretion System ◽  
Effector Proteins ◽  
Enteropathogenic Escherichia Coli ◽  
Type Iii ◽  
Mucosal Surfaces ◽  
Enterocyte Effacement ◽  
Bacterial Cytoplasm

Enteropathogenic Escherichia coli (EPEC) are extracellular pathogens that colonize mucosal surfaces of the intestine via formation of attaching and effacing (A/E) lesions. The genes responsible for induction of the A/E lesions are located on a pathogenicity island, termed the locus of enterocyte effacement (LEE), which encodes the adhesin intimin and the type III secretion system needle complex, translocator and effector proteins. One of the major EPEC translocator proteins, EspA, forms a filamentous conduit along which secreted proteins travel before they arrive at the translocation pore in the plasma membrane of the host cell, which is composed of EspB and EspD. Prior to secretion, many type III proteins, including translocators, are maintained in the bacterial cytoplasm by association with a specific chaperone. In EPEC, chaperones have been identified for the effector proteins Tir, Map and EspF, and the translocator proteins EspD and EspB. In this study, CesAB (Orf3 of the LEE) was identified as a chaperone for EspA and EspB. Specific CesAB–EspA and CesAB–EspB protein interactions are demonstrated. CesAB was essential for stability of EspA within the bacterial cell prior to secretion. Furthermore, a cesAB mutant failed to secrete EspA, as well as EspB, to assemble EspA filaments, to induce A/E lesion following infection of HEp-2 cells and to adhere to, or cause haemolysis of, erythrocytes.

scholarly journals The Plasmid-Encoded Regulator Activates Factors Conferring Lysozyme Resistance on Enteropathogenic Escherichia coli Strains

2008 ◽  
Vol 75 (1) ◽  
pp. 275-280 ◽  
Author(s):  
Nina Salinger ◽  
Bashkim Kokona ◽  
Robert Fairman ◽  
Iruka N. Okeke
Keyword(s):  
Escherichia Coli ◽  
Serine Protease ◽  
Resistance Mechanism ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Enhanced Resistance ◽  
Enterocyte Effacement

ABSTRACT We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on nonpathogenic E. coli, and a second Per-regulated, espC-independent lysozyme resistance mechanism was identified.

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