Molecular Study of Regulatory Gene (Ler) in Enteropathogenic Escherichia Coli (EPEC) of Diarrheagenic Patients

The locus of enterocyte effacement LEE-encoded regulator (Ler( is a global regulator of multiple virulence genes expression in the Enteropathogenic Escherichia coli (EPEC), including those encoding the type III secretion pathway and adhesion proteins such as intimin. Ler is central to the process of the formation of the attaching and effacing (AE) lesions. This study aimed to perform the molecular detection of Ler gene in EPEC, since there is no related previous study in Iraq. Two hundred and fifty stool specimens from children under two years of age for both sexes were collected from some Iraqi hospitals. All isolates were diagnosed according to morphological characteristics and biochemical tests. The results showed that 140 (56%) samples were identified as E.coli, while 8 (5.7%) isolates were identified as EPEC as confirmed by using VITEK 2 system. Susceptibility test was determined for all EPEC isolates to 16 different antibiotics. The results showed that 100%of these isolates were resistant to Ampicillin, Cefazolin, Ceftriaxone, Cefepime, Trimethoprim and Ceftazidime, whereas resistance values to Nitrofurantoin, Cefoxitin and Gentamicin were 66%, 40%, and 15% respectively. However 100% of the isolates were sensitive to Piperacillin, Ertapenem, Imipenem, Amikacin, Ciprofloxacin, Levofloxacin and Tigecycline. Monoplex pattern of PCR was used for detecting 16SrRNA, eae, stx1, lifA and Ler genes in EPEC. The results showed that the isolates of E.coli were positive for 16SrRNA, eae, lifA and Ler, while no bands of stx1 appeared.

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Hierarchy in the expression of the locus of enterocyte effacement genes of enteropathogenic Escherichia coli

Molecular Microbiology ◽

10.1046/j.1365-2958.1999.01655.x ◽

1999 ◽

Vol 34 (5) ◽

pp. 941-952 ◽

Cited By ~ 138

Author(s):

Devorah Friedberg ◽

Tatiana Umanski ◽

Yuan Fang ◽

Ilan Rosenshine

Keyword(s):

Escherichia Coli ◽

Enteropathogenic Escherichia Coli ◽

Enterocyte Effacement

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The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)-encoded regulator (Ler)

Molecular Microbiology ◽

10.1046/j.1365-2958.1999.01473.x ◽

1999 ◽

Vol 33 (2) ◽

pp. 296-306 ◽

Cited By ~ 281

Author(s):

Jay L. Mellies ◽

Simon J. Elliott ◽

Vanessa Sperandio ◽

Michael S. Donnenberg ◽

James B. Kaper

Keyword(s):

Escherichia Coli ◽

Transcriptional Activator ◽

Enteropathogenic Escherichia Coli ◽

Regulatory Cascade ◽

Enterocyte Effacement

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ISOLATION AND MOLECULAR STUDY OF SOME BACTERIAL URINARY TRACT INFECTIONS OF SHEEP IN BASRAH PROVINCE

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i3.1043 ◽

2020 ◽

Vol 51 (3) ◽

pp. 885-893

Author(s):

Mohammed & et al.

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Molecular Detection ◽

Molecular Study ◽

E Coli ◽

Causative Agents ◽

Vitek 2 ◽

Tract Infections

This study was aimed isolation and molecular detection of some causative agents of urinary tract infection (cystitis and pyelonephritis). Out of 108 tested urine samples (56 from females and 52 from males); 60 samples (55.55%) have infected with Escherichia coli and Klebsiella pneumoniae were 36 (64.2%) females and 24 (46.1%) males. The sixty infected samples contain from 56 E. coli and (4) K. pneumoniae, this samples identified by Vitek 2 (44 isolates E. coli and 2 isolates K. pneumoniae) were subjected to DNA extraction. A total of 44 E. coli isolates detected to FimH and pai genes. 44/44 (100%) were positive for presence of FimH gene, and 20/44 (45.45%) were positive for presence of pai gene. The two isolates of K. pneumoniae which detection of Ecpa gene and given positive result to this gene 100%.

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Antibacterial Activity of Zingiber officinale (Ginger) against Clinical Bacterial Isolates

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2019/v3i230080 ◽

2019 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Walla Fadel Mohammed ◽

Bushra Hindi Saleh ◽

Reem Naem Ibrahim ◽

Mohammed Bdaiwy Hassan

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Therapeutic Potential ◽

Morphological Characteristics ◽

Food Preservation ◽

Biochemical Tests ◽

Antibiotic Susceptibility Test

Aims: The objective of the present study was to investigate the phytochemical constituents and antibacterial activity of ginger extracts against some pathogenic bacteria responsible for Urinary tract infection. Study Design: A total of 35 samples were collected from patients with UTIs and wound infections. Place and Duration of Study: The study was conducted at 2 hospitals in Baghdad from1/7/2017 to 1/9/2017 Methodology: The urine sample was collected using a sterile container, while a swap from the infected wound was also taken. The classical methods for diagnosis pathogenic bacteria in urine and wound are based on culture on different microbiological media including. Blood agar, nutrient agar, then incubated at 37°C for 24 hrs. The diagnostic procedures consisted of direct microscopy observation, Gram staining, Biochemical tests, Catalase and coagulase tests. Results: Results show that 30.55%, 38.8%, 19.46% and 11.11% isolates gave typical morphological characteristics and biochemical test for Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonae and Staphylococcus aureus respectively. Antibiotic susceptibility test reveals that Escherichia coli isolates were 100% sensitive to gentamicin, tetracycline, streptomycine. Pseudomonas aeruginosa isolates reveal that 100% of them were sensitive to gentamicin, Imipenem, ampicillin and streptomycine. Staphylococcus aureus isolates reveal that 100% of them were sensitive to Gentamycine, tetracycline and streptomycine. Klebsiella pneumonae isolates reveal that 100% of them were sensitive to nitrofurantoin and Imipenem. Ginger roots extract at high concentration (250,500 mg/ml) have strong antibacterial activity against pathogenic bacteria (Staphylococcus aureus, Escherichia coli and Klebsiella pneumonae). Conclusion: This study has shown that ginger extracts possess medicinal properties, antibacterial activity and that the inhibition of bacterial growth was dose dependent. The results of the present study show that ginger extracts are more effective against all tested bacterial strains. The results of present study have provided the justification for therapeutic potential of ginger and also used as dietary supplement for food preservation.

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Positive effects of multiple pch genes on expression of the locus of enterocyte effacement genes and adherence of enterohaemorrhagic Escherichia coli O157 : H7 to HEp-2 cells

Microbiology ◽

10.1099/mic.0.27100-0 ◽

2004 ◽

Vol 150 (7) ◽

pp. 2357-2571 ◽

Cited By ~ 92

Author(s):

Sunao Iyoda ◽

Haruo Watanabe

Keyword(s):

Escherichia Coli ◽

Genomic Library ◽

Regulatory Gene ◽

Effector Proteins ◽

Multicopy Plasmid ◽

Positive Effects ◽

Enterohaemorrhagic Escherichia Coli ◽

Dna Fragment ◽

Enterocyte Effacement ◽

Positive Effect

Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC, respectively) genomes contain a pathogenicity island, termed the locus of enterocyte effacement (LEE), which encodes genes involved in the formation of attaching and effacing lesions on epithelial cells. To elucidate the regulatory mechanism of the LEE genes in EHEC, an EHEC O157 genomic library was screened for clones which modulated expression of the LEE genes. From more than 5000 clones, a DNA fragment was obtained containing a perC homologue as a positive regulator for the LEE genes. In EPEC, perC is known to be part of the per operon, along with perA and perB, located on the EPEC adherence factor plasmid, which is not found in EHEC. However, the complete genome sequence of EHEC O157 Sakai strain reveals that there are five perC-like sequences, but no perA and perB, on the chromosome. These five perC homologues were characterized, and it was found that three of the homologues (renamed perC homologue pchA, pchB and pchC) encoded 104 aa proteins, and when expressed on a multicopy plasmid enhanced the expression of LEE genes. In contrast, perC homologues encoding proteins of 89 and 90 aa, renamed pchD and pchE, respectively, had no significant effect. Deletion mutants of the pch genes were constructed, and the effect on the expression of LEE-encoded type III effector proteins, such as EspA, B and D, and adhesion phenotype to HEp-2 cells was examined. Deletion of pchA or pchB, but not pchC, decreased the expression of Esp proteins and adhesion to HEp-2 cells. Such effects were more apparent with mutants carrying double deletions of pchA/pchB or pchA/pchC, suggesting that pchA/B/C are all necessary for full expression of the LEE genes and adhesion to HEp-2 cells. Further study demonstrated that the positive effect of pchA/B/C was caused by enhanced transcription of the LEE-encoded regulatory gene, ler. Introduction of a multicopy plasmid carrying each pchA/B/C gene significantly induced microcolony formation by EHEC O157 on HEp-2 cells. These results suggest that the pchABC genes are necessary for full virulence of EHEC O157.

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